Method for preparing chromosome specimen with sepia esculenta embryonic cell

Abstract

A method for preparing a chromosome specimen with a sepia esculenta embryonic cell, which includes the operating procedures of stripping, pre-treating, carrying out hypotonic treatment, fixing, dissociating, dripping, dyeing and washing, and is characterized in that the dripping adopts thermal dripping at the temperature ranging from 40 DEG C to 60 DEG C, the height in the dripping ranges from 1.8mto 2m, and absorbent paper is used for absorbing redundant liquid at a middle position of each liquid drop after each liquid drop is dripped. By means of the thermal dripping, the temperature of thedripping and the height in dripping are reasonably controlled, the chromosome production time is shortened, the chromosome production effect is improved, and the problems of difficulty in mastering of cell rupture degree, chromosome loss, poor chromosome morphology and the like when the chromosome specimen is prepared are solved. The method is used for the chromosome production with the sepia esculenta embryonic cell, so that the good chromosome specimen is obtained, and the chromosome is multiple in phases in metakinesis, clear in morphology and fine in dispersion. Specific effects are indicated in a figure 1.

Description

Technical field: [0001] The invention belongs to the field of cell biology, and in particular relates to a method for preparing a chromosome sample by using golden cuttlefish embryo cells. Background technique: [0002] Chromosomal specimen preparation has the characteristics of simple operation, low price, and low requirement for experimental site. Steps such as slices have been widely used in many fields of biogenetics. However, due to the difficulty in obtaining mature individuals of golden squid, the high cost, and the poor effect of making chromosome samples, it is not satisfactory to use mature individuals to prepare chromosomes. As one of the materials for preparing chromosome specimens, embryos are relatively easy to obtain and low in cost, and are widely used in the preparation of chromosome specimens. In the process of preparing chromosome specimens from golden squid embryos, the conventional cold drop slide method is often used, and it takes a long time for slide...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to provide a method that can make good use of golden squid embryos to prepare chromosome samples. The method uses the hot drop method to reasonably control the temperature of the drop sheet and the height of the drop sheet, shortening the time required for the preparation of the sheet. time, improved the effect of chromosome preparation, and solved the difficulties in grasping the degree of cell rupture, chromosome loss, and poor chromosome shape when preparing chromosome samples.

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