Application of momordica grosvenori proteolytic enzyme in preparing polypeptide and amino acid with protolysate

A protease hydrolysis and protein hydrolysis technology is applied in the application field of Luo Han Guo protease in the preparation of polypeptides and amino acids by hydrolyzing protein, which can solve the problems of low degree of hydrolysis, limited enzyme activity, heavy bitterness of hydrolyzed products, etc. Few, safe effects

Inactive Publication Date: 2008-06-11
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used plant proteases are papaya and bromelain, but the activity of the enzyme is limited, the degree of hydrolysis is low, and some hydrolyzates have a strong bitter taste.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Use soybean protein isolate or soybean meal containing soybean protein to prepare a soybean protein solution with a concentration of 3%, and after treating at 85°C for 15 minutes, adjust the pH value to 8.0;

[0021] (2) Cool to 50°C and add Luo Han Guo protease or fresh Luo Han fruit juice containing Luo Han Guo protease to make the enzyme concentration 1500U / g (protein), keep at 50°C for enzymolysis reaction, and continuously add 0.1mol / sodium hydroxide solution at the same time, Keep the pH value of the soybean protein solution constant and react for 60 minutes;

[0022] (3) After the reaction, rapidly heat to 90°C to terminate the enzyme reaction;

[0023] (4) filtering to obtain soybean polypeptide clarified liquid;

[0024] (5) The above-mentioned filtrate is processed by known methods such as ultrafiltration, column chromatography, and freeze-drying to obtain soybean polypeptide and amino acid products.

Embodiment 2

[0026] (1) Prepare a 3% corn protein concentrate with zein, and adjust the pH value to 7.5-9.5 after treating at 100° C. for 10 minutes;

[0027] (2) Add Luo Han Guo protease to keep the enzyme concentration at 500U / g and keep the protein at 45-65°C for enzymolysis reaction. At the same time, continuously add sodium hydroxide concentrated solution to keep the pH value of the protein concentrated solution unchanged, and react for 120 minutes;

[0028] All the other are with embodiment 1.

Embodiment 3

[0030] Use casein or egg protein to prepare a protein concentration of 3%, and the added mogrosin protease has an enzyme concentration of 1000U / g, and the rest are the same as in Example 1 or Example 2.

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PUM

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Abstract

The invention discloses the application of grosvenor momordica protease in preparing polypeptide and amino acid through protein hydrolysate, the grosvenor momordica protease or fresh grosvenor momordica juice which contains the grosvenor momordica protease is used as an enzymatic agent to add into protein solution with a certain concentration, after controlling the PH valve, temperature, time and termination enzyme reaction of enzymatic hydrolysis, polypeptide supernatant which is filled is obtained, and then polypeptide and amino acid products are capable of being obtained through being treated by known methods such as ultrafiltration, chromatography and drying and the like. Using the grosvenor momordica protease to hydrolyze soybean protein, the problems that the degree of hydrolysis of soybean polypeptide is low and the taste is bitter are further capable of being solved. The grosvenor momordica protease hydrolyzes protein to prepare the polypeptide and the amino acid, the process is simple, the operation is easy, the cost is low, and the commercial process is convenient.

Description

Technical field: [0001] The present invention relates to the application of Luo Han Guo protease, in particular to the application of Luo Han Guo protease in hydrolyzing protein to prepare polypeptide and amino acid. Background technique: [0002] Peptides have good nutrient absorption, excellent physiological functions and processing characteristics, and have better application value than unhydrolyzed proteins. The preparation of polypeptides by enzymatic hydrolysis is one of the important methods to obtain polypeptides and amino acids. Plant proteases are generally used for enzyme preparations, because plant proteases are greener, more environmentally friendly and safer to eat than enzymes from other sources. The commonly used plant proteases are papaya and bromelain, but the activity of the enzyme is limited, the degree of hydrolysis is low, and some hydrolyzed products have a strong bitter taste. Invention content: [0003] The purpose of the invention is to disclose ...

Claims

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Application Information

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IPC IPC(8): A23J3/34
Inventor 苏小建梁成钦何星存
Owner GUANGXI NORMAL UNIV
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