Grosvenor momordica proteinase and its extraction method

An extraction method, the technology of Luo Han Guo, applied in the field of plant protease

Inactive Publication Date: 2006-05-03
GUANGXI NORMAL UNIV
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Problems solved by technology

For a long time, my country's plant protease resources have mainly relied on a few tropical plants such as

Method used

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Embodiment Construction

[0011] Utilize ripe fresh Luo Han Guo fruit to extract and isolate Luo Han Guo protease, the specific process steps are:

[0012] (1) Crude enzyme extraction: select 1000g of fresh Luo Han Guo, wash and drain, squeeze to extract juice, filter cloth, centrifuge (4000r / min, 15min), collect the supernatant, and the supernatant can be obtained by the following three methods: Enzyme powder. Method 1: Filter the supernatant with a Φ150mm 0.45um microporous membrane, and ultrafilter the filtrate through a hollow tube ultrafilter to obtain a cut-off ultrafiltrate. The ultrafiltrate is frozen below -40°C and then vacuum frozen. Drying with a drier, the white powder obtained is 15.8 g of crude enzyme powder; method 2: add pre-cooled ethanol to the clear liquid to 70% (v / v), let stand for a period of time, filter and remove the solution, and the resulting precipitate is vacuum freeze-dried , the light yellow powder obtained is 20.5 g of crude enzyme powder; method three: add ammonium su...

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Abstract

The invention discloses a protease of Momordica grosvenori Swingle as well as its extract method, which is prepared by: with fresh fruit, extracting juice, filtering, ultrafiltrating or salting-out chromatography, depositing with organic solvent, vacuum freezing or spray drying, and obtaining the coarse powder; dissolving the powder with distilled water for column chromatography, dialyzing to desalt, freeze drying, and obtaining the objective product. This product has strong hydrolytic activation to casein; the activity of coarse enzyme can achieve 1.5 million units/g and about two times to coarse papain. This invention adds one plant protease resource, makes our rotease resource do not depend on only pawpaw and pineapple, and has important meaning to enzymology research, protease food development, and other opposite fields.

Description

(1) Technical field: [0001] The invention belongs to plant protease, in particular to protease of Luo Han Guo and its extraction method. (two) background technology: [0002] Luo Han Guo is a unique cucurbit plant in my country. According to reports, Luo Han Guo contains 6-38% of sugars that are easily absorbed by the human body and 1-4% of sweeteners that are 300 times sweeter than sucrose. It is also rich in vitamin C and protein. It has high economic value and edible value. The development and utilization of Luo Han Guo has aroused extensive attention of scientists at home and abroad. [0003] At present, Luo Han Guo is mainly sold as dried fruit. The in-depth development and utilization are mainly the extraction of Luo Han Guo sweetener and health food such as Luo Han Guo Granules, Luo Han Guo Zhen, and Luo Han Guo Drinks made from Luo Han Guo extracts. For a long time, my country's plant protease resources mainly depend on a few tropical plants such as papaya, pineapp...

Claims

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Application Information

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IPC IPC(8): C12N9/50
Inventor 苏小建梁成钦张健李俊何星存
Owner GUANGXI NORMAL UNIV
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