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Method for identifying goose variety

A variety and sample technology, applied in the field of molecular biology, can solve the problems of low resolution, poor reliability, indistinguishable, etc., and achieve the effect of good repeatability and low cost

Inactive Publication Date: 2011-03-16
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above methods have limitations to varying degrees, such as morphological characteristics are easily affected by the environment, and the reliability is poor; the resolution of isoenzyme and SSR markers is low, and it is often impossible to distinguish between closely related varieties, and their repeatability is also poor.

Method used

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  • Method for identifying goose variety
  • Method for identifying goose variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] 1. Construction of goose STR standard genetic map

[0034] (1) Extraction of genomic DNA of different goose breeds (sample size)

[0035] Nine local goose breeds were obtained from the National Waterfowl Germplasm Resource Gene Bank, and 60 goose were randomly selected from each breed, including 30 males and 30 females. 9 goose breeds (Wanxi White Goose, WX; Zhejiang East White Goose, ZD, Sichuan White Goose, SC; Taihu Goose, TH; Lion Head Goose, ST; Huoyan Goose, HY; Wugang Copper Goose, WG; Ma Gang Goose, MG and Black Mane Goose, WZ). 2-3ml of blood was collected from the lower vein of each goose wing, anticoagulated with 0.5% heparin sodium, placed in an ice box and brought back to the laboratory, centrifuged at low temperature and low speed for 5 minutes to carefully remove the upper plasma, and the blood cells were preserved at -20°C.

[0036] ①Take 20ul of red blood cells into a 1.5mL Eppendorf tube, add 500mL poultry blood lysate, 5μL RNase A (20μg / μL), 10μL 10...

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Abstract

The invention discloses a molecular biological method for identifying goose variety. Based on the known research reports, polymorphic abundant microsatellite primers are screened, the individual of different goose varieties is scanned by an ABI 3730XLDNA full-automatic gene analyzer serving as a platform by combining the fluorescence-multiplex PCR technology and adopting the GeneScan-500 standard so as to obtain specific alleles of different varieties and construct a goose STR standard genetic map; and the variety authenticity of a sample to be detected is identified by comparing the STR map of the sample to be detected with a standard map. The method has the advantages of high yield, low cost, quickness, convenience and the like, is a reliable technical approach for scientifically evaluating and identifying the hereditary property of goose variety, and provides technical support for protecting goose breeding property and standardizing the breeding goose market.

Description

technical field [0001] The invention relates to a method for identifying goose varieties, in particular to a method for identifying goose varieties through a microsatellite fluorescent marker automatic genotyping technique, and belongs to the technical field of molecular biology. Background technique [0002] my country is a big goose-raising country in the world, and its annual output accounts for 70% of the world's total. my country is also one of the countries with the richest goose breed resources in the world, with 26 local breed resources. In recent years, with the continuous development of the market economy, the goose Rapid and efficient goose breed identification methods are urgently needed in fields such as production and management, new breed breeding, breed rights protection, and circulation processing. Currently, identification of poultry breeds mainly includes morphological identification, isozyme identification, and simple sequence repeat (SSR) identification. W...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 陈国宏徐琪乔娜段修军赵文明张扬
Owner YANGZHOU UNIV
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