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Method for artificially cultivating resveratrol-enhanced polygonum cuspidatum through tissue culture

A technology of resveratrol and tissue culture, applied in the fields of botanical equipment and methods, horticulture, application, etc., can solve the problems such as the reduction of root growth and excavation, the seeds of Polygonum cuspidatum rarely mature and germinate into seedlings, etc. The effect of production costs

Active Publication Date: 2015-07-01
成都市三禾田生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the wild resources of Polygonum cuspidatum are becoming increasingly depleted. Although there are artificial cultivations, Polygonum cuspidatum mainly relies on rhizome propagation and seed propagation, and rhizome propagation will easily cause the growth of roots and the amount of excavation to decrease; seed propagation, according to field observations, the seeds of Polygonum cuspidatum rarely mature and germinate Seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Site selection and preparation:

[0028] It is advisable to choose the environmental conditions with warm climate, good rainfall or convenient irrigation; sandy soil with fertile, deep soil layer and rich humus content. Before planting, the soil should be plowed 40cm deep to remove impurities such as weeds and tree roots, and holes should be dug according to the row spacing of 90cm*35cm*35cm. Soil and mix well. spare.

[0029] (2) Outer rooting of Polygonum cuspidatum tissue culture seedlings:

[0030] Select the robust and uniformly growing Polygonum cuspidatum tissue culture subculture cluster buds in the patent "201410526530.x", soak the cut single buds in NAA with a concentration of 50mg / L for 5 minutes, and then cut them until they are mixed with 50% 1000 times carbendazim Bacteria-killing matrix (vermiculite: river sand: peat soil = 1:1:2). When the height of the seedlings is about 5cm, the true leaves are 10-15, the roots are 5-6, and the root length is 4...

Embodiment 2

[0042] (1) Site selection and preparation:

[0043]It is advisable to choose the environmental conditions with warm climate, good rainfall or convenient irrigation; sandy soil with fertile, deep soil layer and rich humus content. Before planting, the soil should be plowed 40cm deep to remove impurities such as weeds and tree roots, and holes should be dug according to the row spacing of 90cm*35cm*35cm. Soil and mix well. spare.

[0044] (2) Outer rooting of Polygonum cuspidatum tissue culture seedlings:

[0045] Select the strong and uniform growth of Polygonum cuspidatum from the patent "201410526530.x" to subculture and subculture the clustered shoots. After soaking the cut single buds in NAA with a concentration of 100mg / L for 8 minutes, the cuttings are cut with 50% 1000 times carbendazim Bacteria-killing matrix (vermiculite: river sand: peat soil = 1:1:2). When the seedling height is about 10cm, the true leaves are 10-15, the roots are 5-6, and the root length is 4-5c...

Embodiment 3

[0057] (1) Site selection and preparation:

[0058] It is advisable to choose the environmental conditions with warm climate, good rainfall or convenient irrigation; sandy soil with fertile, deep soil layer and rich humus content. Before planting, the soil should be plowed 40cm deep to remove impurities such as weeds and tree roots, and holes should be dug according to the row spacing of 90cm*35cm*35cm. Soil and mix well. spare.

[0059] (2) Outer rooting of Polygonum cuspidatum tissue culture seedlings:

[0060] Select the robust and uniform growth of Polygonum cuspidatum from the patent "201410526530.x" to subculture and subculture the clustered buds. After soaking the cut single buds in IBA with a concentration of 100mg / L for 10 minutes, the cuttings are cut to 50% 1000 times carbendazim Bacteria-killing matrix (vermiculite: river sand: peat soil = 1:1:2). When the height of the seedlings is about 8cm, the true leaves are 10-15, the roots are 5-6, and the root length is...

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Abstract

The invention relates to the field of a plant planting technology and particularly relates to a method for artificially cultivating resveratrol-enhanced polygonum cuspidatum through tissue culture. The method comprises the following steps of (1) selecting and neatening a cultivating land; (2) performing ex vitro rooting of polygonum cuspidatum tissue culture seedling; (3) field planting; (4) performing field management; (5) controlling pests; (6) harvesting and processing. Polygonum cuspidatum seedlings mainly prepared by a plant tissue culture technique are regular, excellent in quality, and steady in hereditary property. According to the ex vitro rooting of the tissue culture seedling, the seedling raising period is shortened and the product cost is reduced; in the entire process, pesticides and heavy metals are nearly absent, thereby effectively ensuring the quality of polygonum cuspidatum; for the polygonum cuspidatum planted by the method, the effective active ingredients include 0.464-0.532% of resveratrol and 2.651%-2.853% of polydatin and are obviously increased when being compared with those of the wild polygonum cuspidatum; in comparison with an ordinary artificial cultivation method, the acre yield reaches 2000kg is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the field of plant planting, in particular to an artificial planting method of Polygonum cuspidatum obtained by tissue culture with high content of resveratrol. Background technique [0002] Polygonum cuspidatum is a perennial herb of Polygonaceae Polygonum cuspidatum, also known as Yinyang lotus, Huoxuelong, acid stalk, etc. It is produced in southern Shaanxi, southern Gansu, East China, Central China, South China, Sichuan, Yunnan and Guizhou, as well as Korea and Japan. The rhizomes are used for medicine, and have the effects of promoting blood circulation, dispelling blood stasis, stimulating menstruation, and antitussive. The rhizome of Polygonum cuspidatum is rich in polydatin, resveratrol, emodin, chrysophanol, quercetin rhein, quercetin-3-arabinoside, quercetin-3-galactoside, etc., and its extracts are widely used In the fields of medicine, health products, cosmetics, etc., the i...

Claims

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Application Information

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IPC IPC(8): A01G1/00
Inventor 洪汉君熊小灿
Owner 成都市三禾田生物技术有限公司
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