Rejuvenation technology of agricultural microorganism strains by using chlorine dioxide

Abstract

The invention discloses a rejuvenation technology of agricultural microorganism strains by using chlorine dioxide, which comprises the following steps: firstly, microscopic examination is conducted on a shaking flask culture solution of bacteria to be rejuvenated; when in logarithmic growth phase, adding CLO2 solution so as to cause the concentration of CLO2 in the whole culture solution to reach 40 to 120ppm and diluting with aseptic water after shaking treatment are conducted; bacteria suspension containing remained vigorous thalli is coated on a flat plate for culturing and the remained vigorous thalli is examined and enters a culture medium inclined surface until fully distributing the inclined surface; the specific function detection of the strains is conducted and the strains to be improved in respect of specific function to an expected value enter the culture medium inclined surface for culturing and are put in a fridge for later use. The rejuvenation technology adopts chlorine dioxide as a declining individual eliminating agent, is characterized by being safe, changing no hereditary property of the strains, leaving no potential risk and quite obvious restorative improvement on specific function, and has large screening quantity, short and less working procedures, high effectiveness and quite good operational performance.

Description

technical field [0001] The invention relates to a technology for rejuvenating microorganism strains, in particular to a technology for rejuvenating agricultural microorganism strains with chlorine dioxide. Background technique [0002] The methods to prevent the decline and rejuvenation of strains usually include: 1. Control the number of subcultures; 2. Create good culture conditions; 3. Use different types of cells for inoculation and passage. Generally, the above methods are used to prevent the decline of strains. The methods for rejuvenating bacterial strains include: 1. isolation of pure strains; 2. rejuvenation through the host; 3. elimination of decayed individuals. [0003] Although the above-mentioned methods to prevent the decline of bacterial species have relative effects, they have no absolute effect, because the genetic variation of microorganisms is absolute, while stability is relative. Therefore, in order to ensure high quality and high yield, the strain mus...

AI Technical Summary

Problems solved by technology

Although the rejuvenation method of pure species isolation can reach the level of "pure colonies" or even "pure cells", the operation requires a high level, and it is extremely cumbersome, and the workload is heavy; rejuvenation through hosts is only feasible for some parasitic bacteria, and it is very Parasitic bacteria cannot; the rejuvenation technology that eliminates the decayed individuals, such as using -10 to -30°C low temperature treatment of Streptomyces flavinus spores, can preserve the robust individuals of the bacteria, but there is no report that it can be used for other industrial production strains