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Tissue culture method of green chrysanthemums

A tissue culture, green chrysanthemum technology, applied in the field of plant tissue culture, can solve the problems of yellowish color of cluster buds, weak vitality, and long germination time, and achieve the effect of less virus, strong vitality, and short time.

Inactive Publication Date: 2017-12-01
浦江县晶富农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But germination time is long in the above-mentioned method, and the amount of clustered bud is few, and the clustered bud color that grows is yellowish, and vitality is not strong

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The tissue culture method of the green chrysanthemum comprises explant disinfection, callus induction culture, bud differentiation and subculture proliferation culture, rooted and strong seedling culture, hardened seedling transplantation. The time for cultivating green chrysanthemum seedlings by the above method is short, the obtained green chrysanthemum seedlings have less virus and stable genetic properties, the obtained green chrysanthemum branches are thicker, have stronger wind resistance, and their vitality is more vigorous than the green chrysanthemums cultivated by tissue culture in the prior art , a higher survival rate. The steps of shoot differentiation and subculture include transferring the callus to MS medium supplemented with 3.1 mg / LZT, 0.48 mg / L NAA and 0.5 μg / L active short peptide to induce shoot differentiation. The germination time of the callus cultivated by the above method to differentiate into clustered buds is short, the amount of clustered sh...

Embodiment 2

[0028] The tissue culture method of green chrysanthemum, comprises the following steps:

[0029] 1) Disinfection of explants: first rinse the green chrysanthemum bud tip with tap water, then soak it in 10% washing powder for 5 minutes, disinfect it with 70% alcohol for 2 minutes, and use 0.1% HgCl 2 Disinfect for 5 minutes, rinse with sterile water 4 times;

[0030] 2) Induction culture of callus: inoculate the sterilized explants in MS medium with fructose or glucose as carbon source, add BA0.4mg / L and NAA0.2mg / L, and incubate at 25°C under light Cultivate for 12 days under the condition of 2000lx;

[0031]3) Shoot differentiation and subculture: transfer the callus to MS medium supplemented with 3 mg / LZT, 0.5 mg / L NAA and 0.5 μg / L active short peptide to induce shoot differentiation. After 2 days of cultivation at 25°C and 2000lx light, green bumps appeared on the incision, and after 10 days of cultivation, clustered buds appeared, and after 4 days of cultivation, the clus...

Embodiment 3

[0035] 1) Disinfection of explants: first rinse the green chrysanthemum bud tip with tap water, then soak it in 8% washing powder for 6 minutes, disinfect it with 68% alcohol for 2 minutes, and use 0.1% HgCl 2 Disinfect for 5 minutes, rinse with sterile water 4 times;

[0036] 2) Induction culture of callus: Inoculate the sterilized explants in MS medium with fructose or glucose as carbon source, add BA0.36mg / L and NAA0.17mg / L, and incubate at 25°C under light Cultivate for 10 days under the condition of 2500lx;

[0037] 3) Shoot differentiation and subculture: transfer the callus to MS medium supplemented with 2.7mg / LZT, 0.46mg / LNAA and 0.4μg / L active short peptide to induce shoot differentiation. After 3 days of cultivation at 25°C and 2500 lx of light, green bulges appeared on the incision, clustered buds appeared after 12 days of cultivation, and clustered buds grew to 2-4 cm after 4 days of cultivation. Separate the clustered buds at the base of 3 buds, transfer them to...

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Abstract

The invention discloses a tissue culture method of green chrysanthemums. The tissue culture method includes explant sterilization, induced culture of calluses, bud differentiation and subculture enrichment culture, rooting and seedling hardening culture, and acclimatization and transplant. The tissue culture method has the advantages that culture time for green chrysanthemum seedlings is short, the obtained green chrysanthemum seedlings have few viruses and stable hereditary properties, the obtained green chrysanthemum branches are thick, wind resistance is high, and the green chrysanthemums have stronger vitality and higher survival rate than green chrysanthemums subjected to tissue culture in the prior art; active oligopeptides with the amino acid sequences of SCASVCKAHCYCRCGSVFHRGCRCLRC are added into a bud differential medium, and can induce the calluses to re-differentiate, regulate dormancy process, break axillary bud dormancy and shorten budding time, and accordingly, budding efficiency is high; multiplication speed of differentiated cells can be increased obviously, the quantity of multiple shoots is high, and the obtained multiple shoots have large and green leaves and strong vitality.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for tissue culture of green chrysanthemum. Background technique [0002] Green chrysanthemums are popular for their novel colors, but because of the difficulty in growing them, they are generally not common. Green chrysanthemum has no flower seeds, and can only be propagated through vegetative propagation or tissue culture. Most green chrysanthemum varieties grow weakly, and there are not many foot buds and twigs provided for reproduction. The branches are thin and weak, and the survival rate of cuttings is very low. [0003] Plant tissue culture is a technology that separates part of the cells or tissues of the plant from the mother and cultures them under appropriate conditions to enable them to grow, develop, differentiate and proliferate. The principle is from the totipotent differentiation ability of plant cells, that is, a certain type of cells in pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 袁莉霞
Owner 浦江县晶富农业科技有限公司
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