Bacillus amyloliquefaciens capable of producing glutaminase and application thereof

A technology for producing glutaminase and amylolytic spores, which is applied in the direction of enzymes, bacteria, hydrolytic enzymes, etc., can solve the problems of unsuitable glutaminase, complicated operation, unfavorable industrial production, etc., and achieve important industrial utilization value, fermentation The effect of simple conditions and stable genetic properties

Active Publication Date: 2016-07-27
云南博仕奥生物技术有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

This Bacillus amyloliquefaciens produces glutaminase by solid fermentation, but it is not suitable for producing glutaminase by liquid fermentation; solid fermentation efficiency is low, complex operation is unfavorable for industrialized production, and the glutaminase produced by this bacterial strain does not have Performance of high temperature and high salt resistance

Method used

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  • Bacillus amyloliquefaciens capable of producing glutaminase and application thereof
  • Bacillus amyloliquefaciens capable of producing glutaminase and application thereof
  • Bacillus amyloliquefaciens capable of producing glutaminase and application thereof

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Embodiment 1

[0025] Enrichment and seed medium: peptone 5g / L, yeast extract 1g / L, glucose 15g / L, magnesium sulfate 0.1g / L, iron sulfate 0.1g / L, pH7.6~7.8, prepared with hot spring water.

[0026] Fermentation medium: molasses 20g / L, yeast powder 10g / L, calcium chloride 2.8g / L, magnesium sulfate 0.5g / L, manganese sulfate 0.01g / L, sodium chloride 1g / L, disodium hydrogen phosphate 1g / L L, potassium dihydrogen phosphate 1g / L, pH7.5, prepared with deionized water.

[0027] Identification of the Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) BOSAR-X6 bacterial strain

[0028] The activated Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) BOSAR-X6 strain was inoculated in LB medium, cultured at 37°C, 150r / min for 12h, subcultured in LB medium for 2-3 times, and 1.5mL of the end-logarithmic growth The bacterial culture was centrifuged at 10,000 r / min at 4°C for 5 minutes, the bacterial cells were collected, and the culture solution was drained. The bacteria were lysed by SDS and ...

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Abstract

The invention discloses bacillus amyloliquefaciens capable of producing glutaminase and the application thereof and belongs to the field of microorganism screening and fermentation.The strain bacillus amyloliquefaciens BOSAR-X6 is preserved in the China General Microbiological Culture Collection Center on November 30, 2015 with the preservation number of CGMCC No.11760.The culture and fermentation conditions of the strain are simple, hereditary property is stable, the enzyme generated through fermentation has glutamine activity, liquid fermentation efficiency is high, operation is easy, and industrial production can be achieved more easily.Due to the fact that the strain comes from high-temperature hot springs, secreted glutaminase has special characters of salt resistance and high temperature resistance and has important industrial utilization value.

Description

technical field [0001] The invention belongs to the field of microbial screening and fermentation, in particular to a glutaminase-producing Bacillus amyloliquefaciens (Bacillus amyloliquefaciens, BOSAR-X6) and its application. Background technique [0002] Enzymes are macromolecular substances produced by organisms that can act as catalysts, most of which are proteins. Enzyme catalysts not only have obvious high efficiency, but also have catalytic specificity compared with other catalysts. Since microorganisms can secrete almost all enzymes known today, and enzyme preparations derived from microorganisms have irreplaceable advantages over enzyme preparations derived from animals and plants such as papaya and pig pancreas, in order to seek a wider range of microorganisms and activities Material resources, the discovery of new strains and the enzymes they produce are a hot spot in current research. [0003] Glutaminase is a kind of amidase, which is an enzyme that catalyzes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/80C12R1/07
CPCC12N9/80C12Y305/01002C12N1/205C12R2001/07
Inventor 盖作启杜景德崔春黄明媛
Owner 云南博仕奥生物技术有限公司
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