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Aspergillus strain for efficiently degrading nicotine and culturing method and application thereof

A strain and nicotine technology, applied in the field of microorganisms, can solve problems such as unsatisfactory degradation efficiency and intolerance to nicotine

Active Publication Date: 2010-10-20
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, researchers at home and abroad have isolated a variety of nicotine-degrading bacteria from the environment, all of which are concentrated in bacteria. The degradation efficiency is not ideal, and they cannot tolerate higher concentrations of nicotine. Excellent strain for degrading nicotine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0022] The present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0023] The reagents described in the examples are all commercially available products, and the operating methods are common operating methods in the art unless otherwise specified.

Embodiment 1

[0024] Embodiment 1: strain screening

[0025] (1) Separation and purification of tobacco microorganisms

[0026] Weigh 3 grams of the sample (taken from the tobacco leaves during the aging period of the General Group in Jinan, Shandong), put it into a 100mL triangular flask filled with medium, and culture it on a shaker at 150 rpm. Incubate at 28°C for 36-48 hours. The enriched culture solution was made into 10 -1 、10 -2 、10 -3 、10 -4 Suck 0.2mL of the bacterial solution at various dilutions to coat the plate; invert the plate and incubate the bacteria at 37°C for 18-24 hours, and the fungus at 28°C for 36-48 hours; use the plate streaking method to purify, repeat 3 times. Perform microscopic examination on the isolated strains to determine the purity: the bacteria use Staphylococcus aureus (Staphylococcus aureus) and Escherichia coli (Escherichia coli) as controls, and carry out Gram staining. Microscopic examination can distinguish between Gram-positive bacteria and G...

Embodiment 2

[0036] Embodiment 2: strain identification

[0037] Aspergillus oryzae 112822 strain, the preservation number of which is CGMCC NO.3687 in the General Microbiology Center of China Microbiological Culture Collection Management Committee.

[0038] (1) Observation of morphological characteristics

[0039] The spores of Aspergillus oryzae CGMCC NO.3687 were picked with an inoculation loop and placed on the potato medium (PDA) plate and the Chase medium plate respectively, and cultured at 30°C. On the PDA plate, a large number of green spore groups are produced in 2 to 3 days, and the colony spreads; on the Chase plate, the colony grows rapidly, and a large number of green spore groups are produced in 4 to 5 days, and the diameter is 5 to 6 cm in 5 to 7 days. 1 to 2 mm thick, velvety in texture, thicker in the central part of the surface, and the colonies are yellow to turquoise. There are folds on the opposite side of the colony, and the color of the central part gradually deepens...

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Abstract

The invention relates to an aspergillus strain, in particular to the aspergillus oryzae for efficiently degrading nicotine and a culturing method and application thereof, and belongs to the field of microbiological technology. The aspergillus oryzae 112822 strain has been preserved in the normal microorganism centre of China Committee for Culture Collection of Microorganisms on march 29, 2010 with a preservation number as CGMCC No. 3687. The strain has the advantages of simple culturing, stable hereditary property, the capability of degrading the nicotine in the tobacco leach liquor to be 7.8g / L when applied to the degradation of the nicotine and having a broad industrial application prospect.

Description

technical field [0001] The invention relates to an aspergillus strain, in particular to an aspergillus oryzae capable of efficiently degrading nicotine, a culture method and application thereof, belonging to the technical field of microorganisms. technical background [0002] Nicotine is an alkaloid in tobacco. It has a strong stimulating and paralyzing effect on the central nervous system. A small amount can excite people, but a large amount can cause dizziness, vomiting and even poisoning death. Excessive nicotine content in tobacco will seriously endanger the health of smokers. In addition, nicotine is also an environmentally toxic substance. During the production and processing of tobacco products, a large amount of solid, liquid and gas waste will be produced. If these wastes are released into the environment without treatment, they will seriously damage the ecological environment and endanger the environment. human health. Although there are currently some methods fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/69A62D101/26
Inventor 肖敏孟祥璟卢丽丽
Owner SHANDONG UNIV
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