Method for realizing in-vitro preservation of cattleya germplasm resources and recovering growth after preservation

An in vitro preservation and Cattleya technology, applied in the field of plant tissue culture, can solve the problems of large manpower and material resources, cross-contamination, genetic variation, etc., and achieve the effect of prolonging the storage time, strong regeneration ability, and stable genetic genes

Inactive Publication Date: 2017-08-08
SHANDONG CROP GERMPLASM CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Frequent in vitro subculture preservation technology may cause cross-contamination during each subculture process, and multiple subcultures will also cause genetic variation. manpower and material resources

Method used

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  • Method for realizing in-vitro preservation of cattleya germplasm resources and recovering growth after preservation
  • Method for realizing in-vitro preservation of cattleya germplasm resources and recovering growth after preservation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] A method for in vitro preservation of Cattleya germplasm resources and recovery of growth after preservation, the specific steps are as follows:

[0027] (1) Peel the shoot tips from the newly grown pseudobulbs of Cattleya, and place them on the surface of the protocorm induction medium supplemented with 0.5 mg / L 6-benzyl adenine in VW medium to cultivate protocorms.

[0028] Among them, the peeling method of the stem tip is as follows: select a pseudobulb with a length of 5cm, wash the surface dust with tap water, peel off the outermost leaf, scrub with alcohol with a volume concentration of 75% for 3 seconds, and put it in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 3 times, air-dried or blotted with filter paper to dry the surface water, and peeled off the shoot tips with a size of about 0.5 mm under a microscope.

[0029] Place the tip of the shoot on the surface of the protocorm induction medium in the test...

Embodiment 2

[0043] A method for in vitro preservation of Cattleya germplasm resources and recovery of growth after preservation, the specific steps are as follows:

[0044] (1) Peel the shoot tips from the newly grown pseudobulbs of Cattleya, and place them on the surface of the protocorm induction medium supplemented with 1 mg / L 6-benzyl adenine in VW medium to cultivate protocorms.

[0045] Among them, the peeling method of the stem tip is as follows: select a pseudobulb with a length of 7cm, wash the surface dust with tap water, peel off the two outermost leaves, scrub with alcohol with a volume concentration of 75% for 4 seconds, and put in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 4 times, air-dried or blotted with filter paper to dry the surface water, and peeled off the shoot tips with a size of about 0.5 mm under a microscope.

[0046] Place the shoot tip on the surface of the protocorm induction medium in the test tube ...

Embodiment 3

[0058] A method for in vitro preservation of Cattleya germplasm resources and recovery of growth after preservation, the specific steps are as follows:

[0059] (1) Peel the shoot tips from the newly grown pseudobulbs of Cattleya, and place them on the surface of the protocorm induction medium supplemented with 0.8 mg / L 6-benzyl adenine in VW medium to cultivate protocorms.

[0060] Among them, the peeling method of the stem tip is as follows: select a pseudobulb with a length of 6cm, wash the surface dust with tap water, peel off the outermost leaf, scrub with alcohol with a volume concentration of 75% for 4 seconds, and put it in a mass concentration of 0.1 % mercuric chloride solution for 20 minutes, rinsed with sterile water for 3 times, air-dried or blotted with filter paper to dry the surface water, and peeled off the shoot tips with a size of about 0.5mm under a microscope.

[0061] Place the shoot tip on the surface of the protocorm induction medium in the test tube or...

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Abstract

The invention discloses a method for realizing in-vitro preservation of cattleya germplasm resources and recovering the growth after the preservation. The method comprises the following steps: stripping a stem tip from a new growing pseudobulb, putting the stem tip on a surface of a protocorm induction culture medium and culturing to obtain a protocorm; carrying out repeated cutting and subculture on the protocorm, so as to reproduce the protocorms with the needed quantity; culturing by adopting an in-vitro preservation culture medium; after the protocorms enter a development and growth phase, culturing in a dark place at 0 to 5 DEG C; transferring the protocorms which are cultured in the dark place into a differentiation culture medium; differentiating the protocorms to generate seedlings. According to the method disclosed by the invention, a cattleya stem tip culture technology is utilized to induce the protocorms and the protocorms are differentiated to obtain the cattleya seedlings. The protocorms are preserved at low temperature and a proper amount of paclobutrazol is added, so that the preservation time can be greatly prolonged and the number of times of subculture is reduced; the hereditary properties of the germplasm resources are effectively kept. After the protocorms are preserved for certain time, the protocorms can be cultured at normal temprature and under illumination conditions, and the growth of the protocorms can be rapidly recovered and the seedlings are differentiated.

Description

technical field [0001] The invention relates to a method for in vitro preservation and recovery of growth of Cattleya germplasm resources after preservation. It belongs to the technical field of plant tissue culture. Background technique [0002] Cattleya hybrida, also known as Cattleya or Cattleya, is a perennial epiphytic herb of Orchidaceae Cattleya, native to tropical America, and is one of the most famous Cattleya in the world. Pseudobulbs are stick-shaped or cylindrical, with 1 to 3 leaves at the top, thick and hard leaves, concave midrib, large and beautiful flowers, bright and rich colors, white, yellow, green, red and purple, etc., one year Open in all seasons, it is known as the "Queen of Orchid". The propagation method is generally division propagation, which can only be carried out once every 3 to 4 years, and the propagation speed is relatively slow. Cattleya seeds are very small and powdery, without endosperm, only a simple embryo, covered with loose, transp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 丁汉凤辛富刚贾文斌王存娥颜廷进
Owner SHANDONG CROP GERMPLASM CENT
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