The invention discloses a tissue culture and rapid propagation method of millettiadielsiana harms. The method comprises the following steps: selecting and treating of an explant, primary culture, differential culture, multiplication culture and rooting culture, thus forming a complete set of millettiadielsiana harms tissue culture and rapid propagation technology. The tissue culture and rapid propagation method has the characteristics of being short in culture period, high in multiplication coefficient, fast in multiplication speed, good in rooting effect, maintaining the excellent hereditary character of mother plant and the like, the factorized rapid seedling of the millettiadielsiana harms can be achieved, the multiplication coefficient can achieve 15.0-18.0, and compared with the tissue culture and rapid propagation of sterile seedlings obtained by taking seed sprouts, single buds and stems with buds as materials, the tissue culture and rapid propagation method is good in multiplication effect, high in multiplication efficiency, the stages of sprouting and growing of seeds into sterile seedlings in the culture media can be reduced, so that the culture period is shortened, thus providing a tissue culture and rapid propagation production technology for the medicinal and appreciable dual-use excellent material.