Preparation method for physalis angulata root differentiation culture solution
A culture medium and technology of bitter beetroot, applied in the field of culture medium, can solve the problems of long rooting time of rooting medium and unsuitability for the cultivation of bitter beetroot, and achieve the effects of high survival rate, simple and feasible preparation method, and low production cost
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Embodiment 1
[0016] The preparation method of bitter root differentiation culture fluid, concrete steps are:
[0017] Get 0.0019 part of plant growth regulator, 0.00014 part of bacteriostat, 1 part of potassium nitrate, 1 part of ammonium nitrate, 0.16 part of magnesium sulfate, 0.13 part of potassium dihydrogen phosphate, 0.22 part of calcium chloride, 0.28 part of trace element, 0.0022 parts of vitamins, 0.0029 parts of amino acids, 0.0006 parts of niacin, 0.11 parts of inositol, 0.2 parts of anti-browning agent, water 980, mechanically stirred until fully dissolved and uniform, then adjusted to pH 5.8, and finally sterilized at 0.15MPa and 121°C After cooling for 22 minutes, put it in an environment of 3°C to obtain the root differentiation culture medium of bitter beetroot. , the degree of callus tissue is light, and it can effectively control the browning of bitter beetle seedlings, so that the bitter beetle seedlings are strong and the leaves are dark green; at the same time, the cul...
Embodiment 2
[0024]The preparation method of the bitter root differentiation culture solution, the composition and parts by weight of the culture solution are: 0.0025 parts of plant growth regulator, 0.00013 parts of bacteriostatic agent, 1.1 parts of potassium nitrate, 0.9 parts of ammonium nitrate, 0.17 parts of magnesium sulfate, diphosphate 0.12 parts of potassium hydrogen, 0.25 parts of calcium chloride, 0.25 parts of trace elements, 0.0025 parts of vitamins, 0.0025 parts of amino acids, 0.0007 parts of niacin, 0.1 parts of inositol, 0.25 parts of browning agent, and 990 parts of water. The plant growth regulator is IBA, IAA and 6-BA, and its weight ratio is 1:0.4:0.15; the bacteriostatic agent is Schisandra chinensis extract and active polypeptide, and its weight ratio is 1:0.5; the trace element is manganese sulfate or zinc sulfate Or boric acid or sodium molybdate or cobalt chloride or potassium iodide; Anti-browning agent is citric acid and gac, and its weight ratio is 1:0.015, and...
Embodiment 3
[0028] The preparation method of bitter root differentiation culture fluid is:
[0029] Get 0.003 part of plant growth regulator, 0.00012 part of bacteriostatic agent, 1.2 part of potassium nitrate, 1 part of ammonium nitrate, 0.18 part of magnesium sulfate, 0.11 part of potassium dihydrogen phosphate, 0.22 part of calcium chloride, 0.3 part of trace element, 0.0022 parts of vitamins, 0.003 parts of amino acids, 0.0008 parts of niacin, 0.1 parts of inositol, 0.3 parts of anti-browning agent, 1000 parts of water, fully dissolve and evenly, then adjust the pH to 5.5-6.5, and finally adjust the pH at 0.1-0.2MPa, 120-130 Sterilize at ℃ for 20-30 minutes, cool and store in an environment of 2-5 ℃ to obtain the root differentiation culture solution of bitter beetroot.
[0030] Above-mentioned plant growth regulator is IBA, IAA and 6-BA, and its weight ratio is 1:0.3:0.1; Antibacterial agent is Schisandra chinensis extract and active polypeptide, and its weight ratio is 1:0.6; Trace ...
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