Tissue culture and rapid propagation method of millettiadielsiana harms
A technology of rapid propagation of twig and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of maintaining hereditary traits, high multiplication coefficient, and shortening the cultivation period
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[0030] A method for rapid propagation of Tachibana fragrans tissue culture, comprising the following steps
[0031] (1) Explant selection and processing:
[0032] Selection of explants: Select the vigorously growing, no pests and diseases of the twig plants as the propagation material, and pick the young shoots and young leaves from the first and second year branches in the middle and late March as the explants.
[0033] The selection of the explant material is the young leaves that have just opened on the young shoots that grow vigorously, have no damage by diseases and insect pests, no mechanical damage, and no lignification.
[0034] Explant treatment: Rinse the retrieved young leaves with a small amount of washing powder and place them in a bottle, seal the mouth of the bottle with a single layer of medical gauze, rinse in tap water for 60 minutes, pour out the water in the bottle, and put the material Place it in an ultra-clean workbench, firstly treat it with 75% alcoho...
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