A kind of method for rapid propagation of T. japonica by tissue culture

Abstract

The invention discloses a tissue culture and rapid propagation method of millettiadielsiana harms. The method comprises the following steps: selecting and treating of an explant, primary culture, differential culture, multiplication culture and rooting culture, thus forming a complete set of millettiadielsiana harms tissue culture and rapid propagation technology. The tissue culture and rapid propagation method has the characteristics of being short in culture period, high in multiplication coefficient, fast in multiplication speed, good in rooting effect, maintaining the excellent hereditary character of mother plant and the like, the factorized rapid seedling of the millettiadielsiana harms can be achieved, the multiplication coefficient can achieve 15.0-18.0, and compared with the tissue culture and rapid propagation of sterile seedlings obtained by taking seed sprouts, single buds and stems with buds as materials, the tissue culture and rapid propagation method is good in multiplication effect, high in multiplication efficiency, the stages of sprouting and growing of seeds into sterile seedlings in the culture media can be reduced, so that the culture period is shortened, thus providing a tissue culture and rapid propagation production technology for the medicinal and appreciable dual-use excellent material.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of japonica japonica, and in particular relates to a method for tissue culture and rapid propagation of japonica japonica. Background technique [0002] Millettia dielsiana Harms, also known as Millettia dielsiana Harms, is a perennial semi-evergreen woody vine of the family Papilionaceae Millettia dielsiana, most of which are still in the wild. Its vines and roots can be used medicinally, which can promote qi, relieve heat, stop bleeding, drive wind and dehumidify, dredge meridians and activate collaterals. The seeds can also be used as oil materials. In gardens, it can be used for greening on scaffolding, rockery, walls, slopes, ground covers, roofs, bonsai, etc. It is a good medicinal and ornamental landscaping plant. In recent years, due to the fact that the thuja vine is not only an important medicinal plant (since the 1970s, it has been widely excavated in Guangdong and Guangxi for t...

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Problems solved by technology

[0004] At present, there is no report on the tissue culture and rapid propagation technology of T. japonica leaves, and this method has the characteristics of convenient material collection, less consumables, short cultivation period, high reproduction coefficient, fast reproduction speed, good rooting effect, and maintenance of excellent genetic traits of the mother plant, etc. , so that the thuja vine can implement industrialized rapid seedling cultivation

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