Rooting culture method for calophyllum inophyllum tissue culture seedling

A cultivation method and a technique for rooting cultivation, which are applied in the field of rooting cultivation of red thick shell tissue culture seedlings, to achieve the effects of simple operation, high transplanting survival rate, and promotion of root growth

Inactive Publication Date: 2010-02-17
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Establishing a rooting method for tissue cultured regenerated seedlings of red thick shell is a key step in the rapid propagation of red thick shell tissue culture test tube seedlings. There has been no relevant report on the rooting method and transplanting technology of Hainan red thick shell tissue cultured seedlings

Method used

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Embodiment 1

[0028] The rooting culture method of the red thick shell tissue culture seedling of the present embodiment, concrete steps are as follows:

[0029] (1) Get 2 centimeters of high red thick shell tissue culture seedlings (no roots), cut the seedlings under aseptic conditions, transfer to culture in the culture bottle that rooting medium is housed, culture bottle is placed in the light incubator and cultivate, The temperature is 28±1℃, the relative humidity is 70±5%, the light condition is 12 hours / day, and the light intensity is 10±1μmol / m 2 s (represents "micromole / square meter × second"); the formula of the rooting medium is: 1 / 2MS+1.0mg / Lα-naphthaleneacetic acid+8.0g / L agar+30g / L sucrose;

[0030] (2) About 4 weeks, induce rooting, and the rooting rate reaches 86.7%. The seedlings that have taken root are transferred to the subculture medium containing activated carbon and cultivated. The formula of the subculture medium is: 1 / 2MS+0.5g / L Activated carbon + 8.0g / L agar + 30g / ...

Embodiment 2

[0033] The rooting culture method of the red thick shell tissue culture seedling of the present embodiment, concrete steps are as follows:

[0034] (1) Get 2 centimeters of high red thick shell tissue culture seedlings (no roots), cut the seedlings under aseptic conditions, transfer to culture in the culture bottle that rooting medium is housed, culture bottle is placed in the light incubator and cultivate, The temperature is 28±1℃, the relative humidity is 70±5%, the light condition is 12 hours / day, and the light intensity is 10±1μmol / m 2 s (represents "micromole / square meter × second"); the formula of the rooting medium is: 1 / 2MS+1.0mg / Lα-naphthaleneacetic acid+8.0g / L agar+30g / L sucrose;

[0035] (2) After the red thick shell seedlings are induced to go out in the rooting medium, the seedlings that have taken root are transferred to the rooting medium and cultivated, and the formula of the rooting medium and the rooting culture conditions are identical with step (1); about 4...

Embodiment 3

[0038] The rooting culture method of the red thick shell tissue culture seedling of the present embodiment, concrete steps are as follows:

[0039](1) Get 2 centimeters of high red thick shell tissue culture seedlings (no root), cut the seedlings under aseptic conditions, transfer to culture in the culture bottle that rooting medium is housed, culture bottle is placed in the light incubator and cultivate, The temperature is 28±1℃, the relative humidity is 70±5%, the light condition is 12 hours / day, and the light intensity is 10±1μmol / m 2 s (represents "micromole / square meter × second"); the formula of the rooting medium is: 1 / 2MS+1.0g / L activated carbon+8.0g / L agar+30g / L sucrose;

[0040] (2) About 8 weeks, the roots were induced, and the rooting rate reached 82.3%. The rooted seedlings were transferred to the subculture medium containing activated carbon and cultivated. The formula of the subculture medium was: 1 / 2MS+0.5g / L Activated carbon + 8.0g / L agar + 30g / L sucrose; the...

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Abstract

The invention discloses a rooting culture method for a calophyllum inophyllum tissue culture seedling. The method comprises the following steps: (1) transferring a calophyllum inophyllum tissue culture seedling into a rooting culture medium to illuminate and culture and inducing rooting; (2) transferring the rooted tissue culture seedling into a subculture medium containing active carbon to culture; and (3) training the seedling in the subculture medium and then transplanting. The invention has simple and easy operation and high rooting rate and transplanting survival rate of 86.7 percent, rapid adventitious root extension and easy achievement of transplanted seedling growth condition, and the plant height can reach about 30cm after culture for 4 months. The invention can effectively shorten induction rooting time and promote root growth, and an induced root has a developed root system and the healthy and strong seedling. The rooting culture adopts a two-step rooting method; compared with the prior one-step rooting method, the invention has better rooting effect.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the tissue culture of red thick shell, in particular to a method for rooting culture of red thick shell tissue culture seedlings. Background technique [0002] The genus Calophyllum (Calophyllum L) is a plant of the family Guttiferae, and it is distributed in Hainan Island of my country, Southeast Asia, India, Africa and other tropical regions. Red thick shell, also known as Hutong and Begonia, has high economic value and a wide range of applications. It has multiple uses such as oil, medicine, wood, ornamental, and ecological protection. [0003] There are 4 species of C. membranaceum Gardn. et Champ. in my country, of which 2 species are distributed in Hainan, namely C. membranaceum Inophyllum Linn. Due to deforestation and land use, the red thick shell only exists in a small range in some areas. There has not been systematic scientific research on the utilizatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 王小菁许德成
Owner SOUTH CHINA NORMAL UNIVERSITY
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