Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources

A technology of LED light source and hosta, which is applied in the field of tissue culture of ornamental plants, can solve the problems of unsatisfactory subculture and rooting effect of hosta tissue culture seedlings, and the efficiency and quality of hosta tissue culture need to be improved, so as to achieve quality improvement and rooting effect Improved effect

Inactive Publication Date: 2017-05-10
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors of the present application found that the effect of red and blue composite light (2RB) on the subculture proliferation and rooting of hosta tissue culture seedlings is still not ideal, and the tissue culture efficiency and quality of hosta under this condition need to be improved

Method used

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  • Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources
  • Method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources

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Experimental program
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Effect test

Embodiment 1

[0023] The tissue culture seedlings in the proliferation culture stage of hosta were used, cut into single buds in the ultra-clean workbench, and the single buds were inoculated into the rooting medium for rooting culture. The rooting medium formula was: 1 / 2MS+IBA 1.0mg / L +2% sucrose + 0.6% agar powder, the temperature of the culture room is 25℃±2℃, and the light time is 12h / d. The tissue culture seedlings used for rooting culture were placed in a culture rack with 8RB composite light quality (red light + blue light = 8:1, peak wavelength of 663 nm + 452 nm), and the photosynthetically active radiation was 48 μmol m. -2 ·s -1 , Rooted tissue culture seedlings were obtained after culturing for 30 days. The rooting rate, root number, root length, plant height, fresh weight, dry weight, chlorophyll content, soluble sugar content and soluble protein content were determined for the rooting seedlings of hosta. See Table 1 for the results.

Embodiment 2

[0025] The tissue culture seedlings in the proliferation culture stage of hosta were used, cut into single buds in the ultra-clean workbench, and the single buds were inoculated into the rooting medium for rooting culture. The rooting medium formula was: 1 / 2MS+IBA 1.0mg / L +2% sucrose + 0.6% agar powder, the temperature of the culture room is 25℃±2℃, and the light time is 12h / d. The tissue culture seedlings used for rooting culture were placed in a culture rack with 4RB composite light quality (red light + blue light = 4:1, peak wavelength of 663 nm + 452 nm), and the photosynthetically active radiation was 48 μmol m. -2 ·s -1 , Rooted tissue culture seedlings were obtained after culturing for 30 days. The rooting rate, root number, root length, plant height, fresh weight, dry weight, chlorophyll content, soluble sugar content and soluble protein content were determined for the rooting seedlings of hosta. See Table 1 for the results.

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Abstract

The invention provides a method for acquiring fragrant plantain lily herb rooting seedlings by LED (light-emitting diode) light sources. The method includes the step: irradiating fragrant plantain lily herb tissue culture seedlings by LED compound light comprising red light and blue light. The wavelength of the red light ranges from 640 nanometers to 680 nanometers, the wavelength of the blue light ranges from 450 nanometers to 480 nanometers, the ratio of the red light to the blue light is (3-9):1, and photo-synthetically active radiation is 20-75 micro-mol*m-2*s-1. Optimal LED light conditions for fragrant plantain lily herb tissue culture are accidentally discovered in a large quantity of experimental research, and accordingly, the fragrant plantain lily herb tissue culture seedlings are subjected to rooting culture under the light conditions. According to the method, rooting effects and quality are remarkably improved.

Description

technical field [0001] The invention relates to a tissue culture technology for ornamental plants, in particular to a method for improving the quality of hosta tissue culture seedlings by using an LED light source. Background technique [0002] Hosta (Hosta sp.) is the general name of the Liliaceae Hosta plants, alias Yuchun stick, Baihexian, etc. It is native to China and Japan and is widely cultivated all over the world. There are many varieties of hosta, which can see both leaves and flowers. It has the characteristics of cold resistance, shade growth, and low soil requirements. As a ground cover plant, it is widely used in landscaping. It is one of the most promising perennial perennial herb flowers. one. Hosta takes at least 3 years from sowing to flowering, and the offspring of seed propagation generally cannot maintain the excellent traits of the parent. The ramet is a commonly used propagation method in production, but the propagation speed is slow, and the annual ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 孙翊殷丽青张永春杨柳燕李心李青竹陈敏敏
Owner SHANGHAI ACAD OF AGRI SCI
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