Culture medium capable of promoting rooting of rape

Abstract

The invention discloses a culture medium capable of promoting rooting of rape. The culture medium is prepared from components as follows: 4.43 g / L MS, 30 g / L sucrose, 8 g / L agar powder, 5 mg / L AgNO3 and 0.1-2 mg / L NAA or IBA with the pH value of 5.8. Through comparison of the rooting effect of rootless seedlings of rape after the rooting culture medium containing 0.1-2 mg / L NAA or IBA is added, 0.1 mg / L NAA is the best addition hormone and has the best concentration for rooting of the rape through screening, after rooting culture is performed for 24 days, the rooting rate is the highest and is92.5%, the number of fibrous roots is the largest, taproots are longer, and rooting is realized at cutting sites. The conditions that hormone added to the culture medium for rooting of the rape is not unified and the rooting efficiency is uneven can be eliminated, and the success rate of transgenic rape can be improved by improving the rooting efficiency of the rape.

Description

technical field

[0001] The invention relates to plant tissue culture technology, in particular to a culture medium for promoting rooting of rapeseed. Background technique

[0002] Rapeseed rape belongs to Brassica genus Brassica and is an important oil crop. Its products can be processed into human edible oil, industrial lubricating oil, and can also be used as green manure and compost. According to reports, between 2014 and 2015, the global rapeseed oil production exceeded 2.698 million tons, making it the third largest oil-producing plant. Rapeseed cultivars with ideal traits can be quickly obtained through transgenic technology, which is now widely used. According to the statistics of the International Agricultural Biotechnology Application Service (ISAAA), the planting area of ​​genetically modified crops reached 185.1 million hectares in 2016, and 24% of the rapeseed planted globally is Transgenic varieties (http: / / www.isaaa.org), the common way to obtain transgenic ra...

Examples

Embodiment 1

[0012] After the seeds are sterilized, they are sown on the seed germination medium (MS medium 2.22 g / L + sucrose 30 g / L + agar powder 8g / L), and after 5 days (when the seedling grows to 8-10 cm), the upper part of the root ( The distance from the natural rooting site is not less than 1 cm, and the upper part of the root is about 6-8 cm, hereinafter referred to as rootless seedlings), in the rooting medium (4.43 g / L MS medium + 30 g / L sucrose + 8 g / L agar powder + 5 mg / L AgNO 3 ) were added 0.1 – 2 mg / L of NAA / IBA two plant hormones (set one without hormone as a control), rooting culture environment: 25 ℃, relative humidity 60%-70%, 2000 lux (lx) light 16 h / day. Compare its rooting effect to rootless seedlings, every group of 40 rootless seedlings, count the rooting situation of rootless seedlings every day, after 24 days, wash away the rooting medium with water, measure the main root length of the regenerated root, the rooting position (the most bottom distance of the rege...

technical field

[0001] The invention relates to plant tissue culture technology, in particular to a culture medium for promoting rooting of rapeseed. Background technique

[0002] Rapeseed rape belongs to Brassica genus Brassica and is an important oil crop. Its products can be processed into human edible oil, industrial lubricating oil, and can also be used as green manure and compost. According to reports, between 2014 and 2015, the global rapeseed oil production exceeded 2.698 million tons, making it the third largest oil-producing plant. Rapeseed cultivars with ideal traits can be quickly obtained through transgenic technology, which is now widely used. According to the statistics of the International Agricultural Biotechnology Application Service (ISAAA), the planting area of ​​genetically modified crops reached 185.1 million hectares in 2016, and 24% of the rapeseed planted globally is Transgenic varieties (http: / / www.isaaa.org), the common way to obtain transgenic ra...

Examples

Embodiment 1

[0012] After the seeds are sterilized, they are sown on the seed germination medium (MS medium 2.22 g / L + sucrose 30 g / L + agar powder 8g / L), and after 5 days (when the seedling grows to 8-10 cm), the upper part of the root ( The distance from the natural rooting site is not less than 1 cm, and the upper part of the root is about 6-8 cm, hereinafter referred to as rootless seedlings), in the rooting medium (4.43 g / L MS medium + 30 g / L sucrose + 8 g / L agar powder + 5 mg / L AgNO 3 ) were added 0.1 – 2 mg / L of NAA / IBA two plant hormones (set one without hormone as a control), rooting culture environment: 25 ℃, relative humidity 60%-70%, 2000 lux (lx) light 16 h / day. Compare its rooting effect to rootless seedlings, every group of 40 rootless seedlings, count the rooting situation of rootless seedlings every day, after 24 days, wash away the rooting medium with water, measure the main root length of the regenerated root, the rooting position (the most bottom distance of the rege...

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