19 results about "Genetically modified canola" patented technology

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

The invention belongs to the technical field of gene engineering, and particularly relates to an application of a BnaA02g04730D gene in improving the salt tolerance of oilseed rape. According to the invention, a high-salt-tolerance germplasm 'Xiangong saline-alkali oil No.1' is obtained by screening, and a key candidate gene BnaA02g04730D responding to salt stress is excavated by combining absolute quantitative transcriptomics analysis. Yeast stress phenotype identification and transgenic rape verification both prove that the gene positively regulates the salt tolerance of rape. Functional verification shows that under the stress of 0.24% NaCl, the SOD activity, the CAT activity and the POD activity of the overexpressed plant are remarkably improved compared with those of a wild type, and the MDA content is remarkably reduced. The invention preliminarily illustrates a molecular mechanism for endowing rape with salt tolerance by regulating and controlling an anti-oxidation defense system and a hormone signal channel. A new perspective is provided for analysis of a salt-tolerant molecular mechanism of the rape, and a gene resource with application value is also provided for molecular breeding of a new salt-tolerant variety.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of a BnPetM gene or a biological material containing the BnPetM gene in regulation and control of plant photosynthesis. A base sequence of the BnPetM gene is a nucleotide sequence as shown in SEQ ID NO: 1, and a coded protein sequence is an amino acid sequence as shown in SEQ ID NO: 2. Research results prove that the BnPetM gene plays a positive regulation role in rape photosynthesis related traits. Transgenosis tests of the BnPetM gene in oilseed rape prove that overexpression of the gene not only greatly improves photosynthesis capacity of transgenic oilseed rape, but also improves seed yield related traits of oilseed rape families, such as plant height and oil content of seeds. Therefore, the BnPetM gene has a good application prospect in improvement of photosynthetic characters of rape and other crops.

The application provides application of Brassica napus gene BnDTX19 in sclerotinia prevention and control, and is obtained by creating transgenic rapeseed to change the disease resistance of rapeseed material. The application constructs the super-expression and RNAi transgenic rapeseed of BnDTX19, firstly discloses the positive regulation function of the gene on sclerotinia resistance, provides the application of the BnDTX19 gene in obtaining high-sclerotinia-resistant rapeseed material by creating super-expression rapeseed, and the application of the BnDTX19-RNAi rapeseed in obtaining sclerotinia-resistant rapeseed material with weakened sclerotinia resistance. The BnDTX19 gene product provided by the application has the function of transporting the key pathogenic factor oxalic acid of sclerotinia to promote disease resistance, and is a new gene resource suitable for creating and breeding new sclerotinia-resistant rapeseed materials and new varieties.

The invention relates to the field of plant genetic engineering, in particular to application of long-chain non-coding RNA lncRNA0410 in regulation and control of drought resistance of brassica napus. According to the invention, a 35S:: lncRNA0410 overexpression vector is constructed, and agrobacterium tumefaciens-mediated genetic transformation is utilized to obtain a transgenic rape material, so that the lncRNA0410 is successfully overexpressed. The drought resistance phenotype analysis is carried out on the overexpressed transgenic material, and the result shows that compared with the wild type, the lncRNA0410 overexpressed plant shows higher survival rate. The invention proposes and proves that the lncRNA0410 has a remarkable positive drought resistance regulation effect in the brassica napus for the first time. The gene can be widely applied to stress resistance improvement and related molecular breeding processes of oilseed rape, and has important theoretical value and application prospect.

The invention provides a sclerotiniose-resistant HIGS-RNAi recombinant vector as well as a construction method and application thereof, and belongs to the technical field of plant genetic engineering. The recombinant vector disclosed by the invention is constructed by recombining an RNA (Ribonucleic Acid) interference fragment of a targeted sclerotinia sclerotiorum pathogenic key gene Ss218 and / or Ss071 with a vector skeleton. The construction method of the recombinant vector comprises the following steps: respectively amplifying to obtain a hairpin forward sequence and a hairpin reverse sequence of a sclerotinia sclerotiorum pathogenic key gene; and connecting the two amplified fragments with a linearized vector subjected to enzyme digestion to obtain a recombinant vector. The recombinant vector constructed by the method can effectively express interfering RNA, so that the transgenic rape shows significantly enhanced resistance to sclerotinia sclerotiorum. The invention provides a new gene resource and an effective technical means for sclerotiniose-resistant breeding of rape.

This invention belongs to the field of genetic engineering technology, specifically relating to the application of the AtHIR4 gene and / or its encoded protein in regulating plant pathogen resistance. The amino acid sequence of the protein encoded by the AtHIR4 gene described in this invention is shown in SEQ ID No. 1. This invention created transgenic Arabidopsis and rapeseed expressing hypersensitivity response-inducible protein 4 (AtHIR4). In the AtHIR4 transgenic Arabidopsis, the chitin-activated ROS burst and phosphorylation of MAPKs were significantly enhanced. Disease resistance test results showed that the AtHIR4 transgenic plants not only exhibited enhanced resistance to the necrotrophic pathogens *Sclerotinia sclerotiorum* and *Botrytis cinerea*, but also significantly increased resistance to the semi-vitrotrophic pathogen *Pseudomonas syringae*. The AtHIR4 transgenic rapeseed also showed significantly enhanced resistance to *Sclerotinia sclerotiorum* and *Botrytis cinerea*.

The invention discloses an application of an autophagy related protein PbATG8 coding gene in prevention and control of clubroot. The application of the coding gene of the plasmodiophora negundo autophagy related protein PbATG8 in improving the clubroot resistance of plants and / or cultivating clubroot-resistant plants is characterized in that the CDS sequence of the coding gene of the plasmodiophora negundo autophagy related protein PbATG8 is as shown in SEQ ID NO.1. The invention also discloses a preparation method of the coding gene of the plasmodiophora negundo autophagy related protein PbATG8. An RNAi vector is constructed by using an HIGS technology, and the RNAi vector is transformed into the rape Zhongshuan No.11. The PbATG8-RNAi transgenic rape is subjected to plasmodiophora brassicae disease resistance experiment, and the result shows that the PbATG8-RNAi transgenic rape has remarkable resistance to plasmodiophora brassicae, so that the protein and the coding gene thereof can provide new gene resources for green prevention and resistance of rape clubroot.

The application provides application of a Brassica napus class receptor gene BnRLP26 in sclerotinia disease prevention and control, and is obtained by creating transgenic oilseed rape to change the disease resistance of the oilseed rape material. The application constructs a BnRLP26 super-expression and CRISPR gene knockout transgenic oilseed rape, first discloses the positive regulation function of the gene on sclerotinia disease resistance, provides application of the BnRLP26 gene in obtaining high-sclerotinia-disease-resistant oilseed rape material by creating super-expression oilseed rape, and application of the BnRLP26-CRISPR gene knockout oilseed rape in obtaining sclerotinia disease resistance weakened sclerotinia disease oilseed rape material. The BnRLP26 gene provided by the application is a new gene resource suitable for creating and breeding new sclerotinia disease-resistant oilseed rape materials and new varieties.

The invention discloses an oilseed rape BnaA03.KNAT1 gene and application thereof in oilseed rape breeding, and belongs to the technical field of plant genetic engineering. Aiming at the problems of low population light energy utilization efficiency and difficulty in adapting to close planting caused by neglect of leaf included angle character in existing rape breeding, the CDS sequence of the identified BnaA03.KNAT1 gene is shown as SEQ ID NO: 1, an overexpression vector of the gene is constructed, and a transgenic plant is obtained by agrobacterium-mediated transformation of rape. Verification shows that after the gene is expressed, the included angle between leaves and a main stem of the transgenic rape can be remarkably reduced, the plant type is compact, the canopy light transmission is improved, the phenotype is stably inherited in multiple generations, a new gene resource and a technical means are provided for ideal plant type breeding of the rape, and the cultivation of a new high-yield rape variety is assisted; the method has important application value for cultivating high-yield rape varieties suitable for high-density planting.

The application provides application of a Brassica napus class receptor gene BnRLP41 in sclerotinia disease prevention and control, and is obtained by creating transgenic oilseed rape to change the disease resistance of the oilseed rape material. The application constructs a BnRLP41 super-expression and CRISPR gene knockout transgenic oilseed rape, first discloses the positive regulation function of the gene on sclerotinia disease resistance, provides application of the BnRLP41 gene in obtaining high-sclerotinia-disease-resistant oilseed rape material by creating super-expression oilseed rape, and application of the BnRLP41-CRISPR gene knockout oilseed rape in obtaining sclerotinia disease resistance weakened sclerotinia disease oilseed rape material. The BnRLP41 gene provided by the application is a new gene resource suitable for creating and breeding new sclerotinia disease-resistant oilseed rape materials and new varieties.

The application provides application of a Brassica napus calcium ion channel gene BnCNGC3 in sclerotinia prevention and control, and is obtained by creating transgenic rapeseed to change the rapeseed material with disease resistance. The application constructs a BnCNGC3 overexpression transgenic rapeseed and a CRISPR gene knockout rapeseed, firstly discloses the positive regulation function of the gene on sclerotinia resistance, provides application of the BnCNGC3 gene in obtaining high-sclerotinia-resistant rapeseed material by creating overexpression rapeseed, and application of the BnCNGC3-CRISPR gene knockout rapeseed in obtaining sclerotinia-resistant rapeseed material with weakened sclerotinia resistance. The BnCNGC3 gene provided by the application is a new gene resource suitable for creating and breeding new sclerotinia-resistant rapeseed materials and new varieties.

A method of modifying fatty acid profiles of canola (Brassica napus) oil are provided. In some embodiments, the method comprises providing a canola plant having a genetic construct in its genome, the genetic construct comprising coding sequences for Umbellularia californica C12:0 specific acyl-ACP thioesterase and Cinnamomum camphora C14:0 specific acyl-ACP thioesterase operatively linked to seed specific promoters. Also provided are genetically modified canola plants, plant cells, and seeds as well as canola oil extracted therefrom. The canola oils have a balanced fatty acid profile that provides health benefits as well as improved oxidative stability over conventional canola oils.

We provide canola seeds that can be grown as a sustainable crop. [Solution] Seeds of the inbreeding canola line NS-B50027-4 are provided, and representative seeds of the said inbreeding canola line are deposited under ATCC accession number PTA-123186.

The invention relates to the technical field of transgenic plants, in particular to a nucleic acid molecule, a primer group and application thereof. The invention provides a herbicide-resistant glyphosate and glufosinate-ammonium rape WYN296 transformation event exogenous insertion flanking sequence, a full-length sequence and application of the herbicide-resistant glyphosate and glufosinate-ammonium rape WYN296 transformation event exogenous insertion flanking sequence in transgenic rape detection, by designing a specific primer pair, whether to-be-detected rape is cp4 epsps and bar transgenic herbicide-resistant rape WYN296 or not can be detected through a PCR method, and the method is high in accuracy, specificity and sensitivity and can be used for detecting the herbicide-resistant rape WYN296. The method can rapidly, efficiently and specifically detect whether a sample to be detected is derived from the transgenic component, provides technical support for market identification and identification after commercialization and industrialization of the product, and has important significance and wide prospects for promoting identification of rape germplasm resources in China.

The application belongs to the technical field of genetic engineering, and particularly discloses a salt-tolerant gene Di19 and application thereof in breeding of oilseed rape with salt tolerance, wherein the gene of Brassica carinata is first transferred into oilseed rape by using transgenic technology, and the transgenic oilseed rape obtained has significantly improved salt tolerance as compared with wild-type oilseed rape. Di19 Therefore, the application provides a new gene resource for breeding of oilseed rape with salt tolerance.

The invention relates to a nucleic acid sequence for detecting rape plant DBN7009 and a detection method thereof. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof. The rape plant DBN7009 has good tolerance to glyphosate, fine glufosinate-ammonium (or glufosinate-ammonium), 2, 4-D and topramezone herbicides and has no influence on yield, and the detection method can accurately and rapidly identify whether a biological sample contains DNA molecules of the transgenic rape event DBN7009 or not.

The invention relates to the technical field of plant genetic engineering, in particular to a rape BnHD33 protein and a coding gene and application thereof. By overexpressing the BnHD33 and the homologous gene of the BnHD33, transgenic oilseed rape with flat leaves, no shrinkage, better firmness, larger branching angle and longer pod length can be obtained, so that the BnHD33 and the homologous gene of the BnHD33 can positively regulate and control the branching angle of the oilseed rape. The invention provides an important theoretical basis and a valuable breeding molecular tool for molecular design breeding of ideal rape plant types, and has a wide application prospect.

Provided is a use of the hypersensitive induced reaction protein 4 (AtHIR4) gene and / or a protein coded thereby in the regulation of plant pathogen resistance, the amino acid sequence of the protein coded by the AtHIR4 gene being as shown in SEQ ID No. I. Transgenic Arabidopsis thaliana and rapeseed are created by means of over-expression of AtHIR4. Resistance of the AtHIR4 transgenic Arabidopsis thaliana to the necrotrophic pathogenic true fungi Sclerotinia sclerotiorum and Botrytis cinerea is enhanced, and disease resistance to the hemibiotrophic pathogenic bacterium Pseudomonas syringae is also significantly increased. The resistance of the AtHIR4 transgenic rapeseed to Sclerotinia sclerotiorum and Botrytis cinerea is also significantly enhanced.