The present invention discloses the guide object sequence and reagent box used for real-time PCR inspection of transgenic rapeseed SYBR Green, providing the guide object sequence for the inspection of extraneous structure gene of transgenic rapeseed RT73 system, extraneous structure gene of MS8 system and endogenous gene of FatA, as well as the maxima, minima and optima selection sequence and the reagent box produced in accordance with guide object sequence. The present technology is used for qualitative and quantitative inspection of transgenic product and has the advantages of high resolution and avoiding cross pollution causing pseudo-positive reaction. SYBR Green real-time PCR technology employs complete closed-tube method, and PCR post treatment is not needed, avoiding cross pollution causing pseudo-positive reaction. SYBR Green dye is also very stable at the extreme temperature demanded by PCR reaction, which can efficiently distinguish specific product, nonspecific product and guide object dipolymer to compensate its bad specificity. The inspection technology is fast, sensitive, simple in operation, time saving and energy saving, accurate and reliable.