Stain-resistant gene Di19 and application thereof in rape stain-resistant breeding

By overexpressing the Di19 gene in rapeseed, the problem of insufficient stain resistance of rapeseed under stain stress was solved, and the stain resistance of rapeseed was significantly improved during the germination period and the high-quality development of the rapeseed industry was promoted.

CN119752945AActive Publication Date: 2025-04-04OIL CROPS RES INST CHINESE ACAD OF AGRI SCI
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Patent Information

Application Number
CN202510096142.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2025-01-03
Filing Date
2025-01-21
Publication Date
2025-04-04
Estimated Expiration
2045-01-21

AI Technical Summary

Technical Problem

In the prior art, rapeseed is susceptible to hypoxic stress and ionic poisoning when facing stain damage stress, which affects its underground root development and above-ground plant growth. At the same time, the disease resistance is weakened, easy to fall, and the oil content of seeds is reduced, which restricts the development of the rapeseed industry.

Method used

By overexpressing the Di19 gene in rapeseed, the Di19 gene was transferred to rapeseed by Agrobacterium transformation method, which improved the stain resistance of rapeseed.

Benefits of technology

It significantly improves the stain resistance of rapeseed during the germination period and can take root and emerge normally in a flooded anaerobic environment. Compared with wild-type rapeseed, the germination rate is 12.6 times to 27 times.

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Abstract

The invention belongs to the technical field of gene engineering, and particularly discloses a waterlogging-resistant gene Di19 and application thereof in waterlogging-resistant breeding of oilseed rape, the Di19 gene of Essel Russian mustard is transferred into oilseed rape for the first time by utilizing a transgenic technology, and the waterlogging resistance of the obtained transgenic oilseed rape is remarkably improved compared with that of wild type oilseed rape. Therefore, the invention provides a new gene resource for breeding the waterlogging-resistant rape.
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