Tissue culture method of conyza blinii

A technology of tissue culture and golden gentian, applied in the field of plant tissue culture, can solve the problems of low transplant survival rate, high pollution rate, high browning rate, etc., achieve optimal sterilization conditions, optimize seedling hardening conditions, and solve the problem of rooting low rate effect

Inactive Publication Date: 2016-07-20
SICHUAN AGRI UNIV
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Problems solved by technology

[0003] In view of this, the present invention is aimed at the above-mentioned problem, provides a kind of Gentiana tissue culture method, has solved the high pollution rate, high mortality rate, high browning rate, low rooting rate and transplanting in the prior art. The problem of low survival

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  • Tissue culture method of conyza blinii
  • Tissue culture method of conyza blinii
  • Tissue culture method of conyza blinii

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[0029] The implementation of the present invention will be described in detail below with reference to the drawings and examples, so as to fully understand and implement the implementation process of how to use technical means to solve technical problems and achieve technical effects in the present invention.

[0030] The invention provides a method for tissue culture of Gentiana gentian, such as figure 1 shown, including the following steps:

[0031] 1) Selection and sterilization of explants: Collect healthy new leaves, old leaves (relatively new leaves), petioles, stem nodes and other tissues and organs that are healthy and free from diseases and insect pests as spare explants; wash the explants under running water After 30 minutes, drain the residual water and place it on ice for 1 hour; after the ice bath, put it into a clean large Petri dish and bring it into the ultra-clean workbench, soak it in 70% alcohol for 5s-30s, and rinse it with a large amount of sterile water i...

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Abstract

The invention discloses a tissue culture method of conyza blinii. The method includes: explant selecting and sterilizing, explant inoculating and culturing, callus induction germination, proliferation and differentiation culturing, root induction, seedling hardening and transplanting. The method has the advantages that the sterilizing conditions are optimized, and the pollution rate and death rate of explant are lowered; hormone types and proportion are adjusted, a certain proportion of antioxidant is added, and the problem that callus is high in browning rate during formation is inhibited effectively; the hormone types and proportion are adjusted, and the problems that differentiated seedlings are long in rooting time and low in rooting rate are solved; the seedling hardening conditions are optimized, the transplanting method is improved, and transplanting survival rate is increased.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture of Gentiana gentiana. Background technique [0002] Golden gentian (Conyzablinii H.Lév.) belongs to the biennial herbaceous plant of the genus Liquor Grass of the Compositae family. parts of western Guizhou. As a medicinal plant, it is cold in nature and extremely bitter in taste. It has the effects of clearing heat, reducing inflammation, detoxifying, removing silt, and reducing swelling. Significant curative effect. The acquisition of Gentiana medicinal materials mainly relies on the collection of wild plants. However, wild species of Gentiana are scattered in a small area, and the collection is difficult and the quantity cannot meet the needs of the medicinal material market and scientific research. Therefore, we need a technology and method for rapid propagation of Gentiana to ensure basic scientific research and market demand. ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 陈惠王涛孙蓉郑天润刘默洋李成磊吴琦刘姗唐自钟何周凤
Owner SICHUAN AGRI UNIV
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