Method for gardenia tissue culture

A tissue culture, gardenia technology, applied in horticultural methods, botanical equipment and methods, gardening, etc., can solve problems such as difficulty in tissue culture

Active Publication Date: 2013-12-11
GUANGZHOU BAIYUNSHAN MINGXING PHARM CO LTD +1
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Problems solved by technology

The tissue culture of woody plants is generally difficult, and there is no report on the callus i

Method used

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  • Method for gardenia tissue culture
  • Method for gardenia tissue culture
  • Method for gardenia tissue culture

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Embodiment Construction

[0014] The present invention selects the pericarp, seed mass and seeds of Gardenia jasminoides as explants, respectively carries out callus induction, differentiation, bud proliferation and rooting culture, thereby evaluating the feasibility of gardenia tissue culture when different parts of the fruit are used as explants sex.

[0015] 1. Plant material

[0016] The pericarp, seed mass and seeds of Gardenia jasminoides are used as explants. Gardenia is collected from Gardenia GAP base in Pingyuan County, Meizhou City, Guangdong Province. Divide green, immature fresh gardenia fruit into pericarp, seed mass (composed of placenta and immature seeds), and seeds, and rinse under tap water until the pigment is washed away (2-3 h). Soak the three kinds of explants together in 70% ethanol for 30 s on the ultra-clean workbench, and then wash them with 0.1% HgCl 2 Soak for 9 minutes, and finally rinse with sterile water for 5-6 times, transfer to sterilized sterile filter paper to ab...

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Abstract

The invention relates to a method for gardenia tissue culture, which comprises the following steps: (a), gardenia seeds are used as explants so as to carry out callus induction; (b), the callus is split in liquid differential medium so as to obtain callus with buds; (c), the callus with buds is cultivated in bud multiplication solid medium so as to obtain more regeneration buds; (d), all the buds are cut off from base parts and transferred to rooting medium for root induction. According to the invention, the gardenia seeds are used as the explants, the callus is induced to form on MS culture medium with different hormone combination; a method of combining solid culture and liquid culture is adopted to induce the differentiation of buds and roots, so as to obtain gardenia regeneration seedlings. In the invention, a tissue culture system taking gardenia reproductive organs as the explants is built, and an experimental platform for further obtaining high effective-component content of gardenia through cell culture and the research of gardenia transgenosis is built.

Description

technical field [0001] The invention relates to a tissue culture method of Rubiaceae medicinal plant gardenia, in particular to a method for tissue culture using immature gardenia seeds as explants. Background technique [0002] Gardenia ( Gardenia jasminoides Ellis) is an evergreen shrub of Rubiaceae. Its dry and mature fruit has the functions of purging fire and relieving restlessness, clearing away heat and diuresis, cooling blood and detoxification (National Pharmacopoeia Commission, 2010), which is included in Part I of Pharmacopoeia of the People's Republic of China (2010 Edition) It is also an important raw material for the production of 33 kinds of Chinese patent medicines such as "Qingkailing" and "Angong Niuhuang Pill". The active ingredients of gardenia are geniposide, crocin, chlorogenic acid, etc., among which crocin is also the main component of natural pigment gardenia yellow. Secondary metabolites can be obtained by culturing plant cells, but the types and...

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Application Information

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IPC IPC(8): A01H4/00
Inventor 何国振张庆红周小琴杨锐培李同根陈红李锦坤
Owner GUANGZHOU BAIYUNSHAN MINGXING PHARM CO LTD
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