Shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema

Abstract

The invention belongs to the technical field of traditional Chinese medicine tissue culture breeding seedling growing, and discloses a shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema. The shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema comprises following steps: material selection; culture medium preparation; tender shoot disinfection; tender shoot inoculation; culture management; and hardening seedling and transplanting. The shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema is simple, and isconvenient in operation; the rooting rate is 94%; the survival rate after transplanting is 96%; seedling growing cost is reduced greatly; tender shoots are taken as a tissue culture material, so thatphotosynthesis can be realized, and dependence on nutrients in the culture medium is reduced; after harvesting, the tender shoots are immersed in a low temperature tranexamic acid solution, so that tender shoot cell division is accelerated, and tender shoot rooting rate is increased; mecobalamin and sodium selenite are added into a MS culture medium, so that growth of competitive bacteria is inhibited, the activity of protoplasm of cells at wounds is increased, callus formation is accelerated, and seedling growing efficiency is increased.

Description

technical field [0001] The invention mainly relates to the technical field of tissue culture propagation and seedling raising of traditional Chinese medicines, in particular to a method for stem tip tissue culture propagation seedling raising of Polygonatum polyflora. Background technique [0002] There are many types of Polygonatum, Polygonatum Polygonatum, Polygonatum Polygonatum, Polygonatum Polyflora and Polygonatum Polyflora, among which Polygonatum Polyflora has the best quality, Polygonatum Polyflora is rich in polysaccharides, oligosaccharides, flavonoids, anthraquinones, saponins and lignans Supplements, etc., are nourishing top grades, which can nourish the lungs and cough, strengthen the spleen and nourish the stomach, resist pathogenic microorganisms, treat diabetes, relieve fatigue, resist oxidation, delay aging, stop bleeding, protect cardiovascular and cerebrovascular, and resist viruses. The understanding of the health care function of Rhizoma Polygonatum, it...

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Problems solved by technology

[0002] There are many types of Polygonatum, Polygonatum Polygonatum, Polygonatum Polygonatum, Polygonatum Polyflora and Polygonatum Polyflora, among which Polygonatum Polyflora has the best quality, Polygonatum Polyflora is rich in polysaccharides, oligosaccharides, flavonoids, anthraquinones, saponins and lignans Supplements, etc., are nourishing top grades, which can nourish the lungs and cough, strengthen the spleen and nourish the stomach, resist pathogenic microorganisms, treat diabetes, relieve fatigue, resist oxidation, delay aging, stop bleeding, protect cardiovascular and cerebrovascular, and resist viruses. The understanding of the health care function of Rhizoma Polygonatum, its demand is also constantly increasing, and the large-scale breeding and planting of Rhizoma Polygonatum has become a development trend; at present, Rhizoma Polygonatum is mostly propagated by rhizomes and seeds, and rhizome propagation is easy to make Rhizoma Polygonatum Loss of good traits, the cycle of seed reproduction is too long, the seeds need to be stored in sand for about 5 months after harvesting, and the rhizomes can fully grow after 4 months after sowing, and the germination rate can only reach about 70%. Excavation, planting efficiency is too low; Therefore Polygonatum multiflora new propagation method becomes research hotspot, document " Polygonatum multiflora tissue culture technology, Zhou Jianjin etc. " has reported that Polygonatum multiflora takes rhizome as material to carry out tissue culture, and Rhizoma Polygonatum is Using rhizomes as Chinese medicinal materials causes a great waste of traditional Chinese medicines, and in the literature, the rhizomes are firstly induced and cultured, and then the callus is formed and then divided, multiplied and cultivated, and then the sprouts are transferred to the rooting medium for rooting Cultivation, repeated segmentation and transfer many times, it is easy to cause the cultured tissue to be contaminated by bacteria, and the operation needs to be performed on an ultra-clean workbench, and a special person is required to perform the cultivation, which wastes a lot of manpower and material resources. Therefore, a new multi- Tissue culture technique of Rhizoma Polygonatum