Shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema

A Polygonatum polyflora, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of bacterial contamination of cultured tissue, waste of manpower and material resources, loss of excellent characters, etc., to avoid infection and rot, Avoid waste, speed up formation and differentiation

Inactive Publication Date: 2018-08-21
ANHUI HUOSHAN COUNTY SONGHETANG TRADITIONAL CHINESE MEDICINE CO LTD
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are many types of Polygonatum, Polygonatum Polygonatum, Polygonatum Polygonatum, Polygonatum Polyflora and Polygonatum Polyflora, among which Polygonatum Polyflora has the best quality, Polygonatum Polyflora is rich in polysaccharides, oligosaccharides, flavonoids, anthraquinones, saponins and lignans Supplements, etc., are nourishing top grades, which can nourish the lungs and cough, strengthen the spleen and nourish the stomach, resist pathogenic microorganisms, treat diabetes, relieve fatigue, resist oxidation, delay aging, stop bleeding, protect cardiovascular and cerebrovascular, and resist viruses. The understanding of the health care function of Rhizoma Polygonatum, its demand is also constantly increasing, and the large-scale breeding and planting of Rhizoma Polygonatum has become a development trend; at present, Rhizoma Polygonatum is mostly propagated by rhizomes and seeds, and rhizome propagation is easy to make Rhizoma Polygonatum Loss of good traits, the cycle of seed reproduction is too long, the seeds need to be stored in sand for about 5 months after harvesting, and the rhizomes can

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for raising seedlings through shoot tip tissue culture of Polygonatum polyflora, comprising the following steps:

[0019] (1) Material selection: Select Polygonatum multiflora with excellent properties, cut the shoots, avoid wasting the rhizomes of traditional Chinese medicine materials, and the leaves of the shoots can perform photosynthesis, reducing the dependence of the shoots on the nutrients in the medium, running water Rinse for 40-60 minutes, place in tranexamic acid solution with a mass concentration of 10mg / L, and soak for 2-3 hours at 6-8°C to accelerate the division of bud cells, promote the formation and differentiation of callus, and improve The rooting rate of tender shoots must be cultivated tender shoots; for the tender shoots, when the distance between the topmost blade and the adjacent blade is 8-10mm, they are cut together with the tender stems, and the length of the tender stems is 8-10mm;

[0020] (2) Culture medium preparation: prepare so...

Embodiment 2

[0026] A method for raising seedlings through shoot tip tissue culture of Polygonatum polyflora, comprising the following steps:

[0027] (1) Material selection: Select Polygonatum multiflora with excellent properties, cut the shoots, avoid wasting the rhizomes of traditional Chinese medicine materials, and the leaves of the shoots can perform photosynthesis, reducing the dependence of the shoots on the nutrients in the medium, running water Rinse for 40-60 minutes, place in a tranexamic acid solution with a mass concentration of 13 mg / L, and soak for 2-3 hours at 6-8°C to accelerate the division of bud cells, promote the formation and differentiation of callus, and improve The rooting rate of tender shoots must be cultivated tender shoots; for the tender shoots, when the distance between the topmost blade and the adjacent blade is 8-10mm, they are cut together with the tender stems, and the length of the tender stems is 8-10mm;

[0028] (2) Culture medium preparation: prepare...

Embodiment 3

[0034] A method for raising seedlings through shoot tip tissue culture of Polygonatum polyflora, comprising the following steps:

[0035] (1) Material selection: Select Polygonatum multiflora with excellent properties, cut the shoots, avoid wasting the rhizomes of traditional Chinese medicine materials, and the leaves of the shoots can perform photosynthesis, reducing the dependence of the shoots on the nutrients in the medium, running water Rinse for 40-60 minutes, place in tranexamic acid solution with a mass concentration of 15mg / L, and soak for 2-3 hours at 6-8°C to accelerate the division of bud cells, promote the formation and differentiation of callus, and improve The rooting rate of tender shoots must be cultivated tender shoots; for the tender shoots, when the distance between the topmost blade and the adjacent blade is 8-10mm, they are cut together with the tender stems, and the length of the tender stems is 8-10mm;

[0036] (2) Culture medium preparation: prepare so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention belongs to the technical field of traditional Chinese medicine tissue culture breeding seedling growing, and discloses a shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema. The shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema comprises following steps: material selection; culture medium preparation; tender shoot disinfection; tender shoot inoculation; culture management; and hardening seedling and transplanting. The shoot tip tissue culture breeding seedling growing method of Polygonatum cyrtonema is simple, and isconvenient in operation; the rooting rate is 94%; the survival rate after transplanting is 96%; seedling growing cost is reduced greatly; tender shoots are taken as a tissue culture material, so thatphotosynthesis can be realized, and dependence on nutrients in the culture medium is reduced; after harvesting, the tender shoots are immersed in a low temperature tranexamic acid solution, so that tender shoot cell division is accelerated, and tender shoot rooting rate is increased; mecobalamin and sodium selenite are added into a MS culture medium, so that growth of competitive bacteria is inhibited, the activity of protoplasm of cells at wounds is increased, callus formation is accelerated, and seedling growing efficiency is increased.

Description

technical field [0001] The invention mainly relates to the technical field of tissue culture propagation and seedling raising of traditional Chinese medicines, in particular to a method for stem tip tissue culture propagation seedling raising of Polygonatum polyflora. Background technique [0002] There are many types of Polygonatum, Polygonatum Polygonatum, Polygonatum Polygonatum, Polygonatum Polyflora and Polygonatum Polyflora, among which Polygonatum Polyflora has the best quality, Polygonatum Polyflora is rich in polysaccharides, oligosaccharides, flavonoids, anthraquinones, saponins and lignans Supplements, etc., are nourishing top grades, which can nourish the lungs and cough, strengthen the spleen and nourish the stomach, resist pathogenic microorganisms, treat diabetes, relieve fatigue, resist oxidation, delay aging, stop bleeding, protect cardiovascular and cerebrovascular, and resist viruses. The understanding of the health care function of Rhizoma Polygonatum, it...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李彪
Owner ANHUI HUOSHAN COUNTY SONGHETANG TRADITIONAL CHINESE MEDICINE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap