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Method used for induction of garlic calluses from garlic cloves

A technology of callus and garlic cloves, which is applied in the field of garlic callus induced by garlic cloves, can solve the problems of difficult breeding work, unfavorable germplasm resources, and inconvenient material collection, so as to avoid the shortage of garlic germplasm resources, wide sources, and convenient material collection Effect

Inactive Publication Date: 2014-02-26
重庆市黔江区阿蓬科技企业孵化器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the stem buds, stem tips or embryos of garlic bulbs are generally used as explants to induce garlic to regenerate; although the above-mentioned explants can successfully induce callus, it needs to be at the cost of losing a large amount of bulbs. , is not conducive to the preservation of germplasm resources, and the volume of the stem tip or stem bud is small, which makes it inconvenient to obtain materials, which increases the difficulty of breeding work

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Explant treatment: Peel the garlic and wash the garlic cloves with sterile water, soak them in ethanol with a volume concentration of 75% for 30 seconds, then sterilize them in sodium hypochlorite with a volume concentration of 3% for 8 minutes, and clean them with sterile water Finally, cut off the top and base of the garlic cloves in a sterile operating table, and use the middle part of the garlic cloves as explants, first cut the explants into two tissue blocks on average in the longitudinal direction, and then cut each tissue block into two parts vertically organization block;

[0017] (2) Inoculation: Randomly inoculate the tissue blocks in step 1 into the induction medium. When inoculating, the part of the tissue block that was originally at the base of the garlic clove is in contact with the medium, and a total of 100 tissue blocks are inoculated; the induction medium is MS medium, 2mg of NAA, 1mg of 6-BA, 0.5mg of ZT, 25g of sucrose and 7g of agar are added ...

Embodiment 2

[0020] (1) Explant treatment: Peel the garlic and wash the garlic cloves with sterile water, soak them in ethanol with a volume concentration of 75% for 30 seconds, then disinfect them in 5% sodium hypochlorite for 3 minutes, and wash them with sterile water After cleaning, cut off the top and base of the garlic cloves in an aseptic operating table, and use the middle part of the garlic cloves as explants. Cut the explants into two tissue blocks on average in the longitudinal direction, and then cut each tissue block into two pieces in the longitudinal direction. Part organization block;

[0021] (2) Inoculation: Randomly inoculate the tissue blocks in step 1 into the induction medium. When inoculating, the part of the tissue block that was originally at the base of the garlic clove is in contact with the medium, and a total of 100 tissue blocks are inoculated; the induction medium is MS medium, 2mg of NAA, 1mg of 6-BA, 0.5mg of ZT, 25g of sucrose and 7g of agar are added to 1...

Embodiment 3

[0024] (1) Explant treatment: Peel the garlic and wash the garlic cloves with sterile water, soak them in ethanol with a volume concentration of 75% for 30 seconds, then sterilize them in sodium hypochlorite with a volume concentration of 3% for 8 minutes, and clean them with sterile water Finally, cut off the top and base of the garlic cloves in a sterile operating table, and use the middle part of the garlic cloves as explants, first cut the explants into two tissue blocks on average in the longitudinal direction, and then cut each tissue block into two parts vertically organization block;

[0025] (2) Inoculation: Randomly inoculate the tissue blocks in step 1 into the induction medium. When inoculating, the part of the tissue block that was originally at the base of the garlic clove is in contact with the medium, and a total of 100 tissue blocks are inoculated; the induction medium is MS medium, 2mg of NAA, 1.5mg of 6-BA, 1mg of ZT, 25g of sucrose and 7g of agar are added ...

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Abstract

The invention discloses a method used for induction of garlic calluses from garlic cloves. The method comprises following steps: garlic cloves are disinfected, and are longitudinally cut so as to obtain tissue blocks; and an induction medium is inoculated with the tissue blocks, and induction of formation of the garlic calluses is realized under dark conditions, wherein an induction temperature is kept at 18 to 22 DEG C. The induction medium is MS medium, and 1 to 2mg of NAA, 1 to 1.5mg of 6-BA, 0.5 to 1mg of ZT, 20 to 30g of sucrose and 7g of agar are added into 1L of MS medium. According to the method, the garlic cloves are taken as explants, material selecting is convenient, the method is simple and convenient, and inductivity can reach 88%.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing garlic callus by using garlic cloves. Background technique [0002] garlic( Allium sativum L.Garlic) is a perennial herb belonging to the Allium genus of Liliaceae; the underground bulbs are divided into petals, spicy and have a pungent smell, and can be used for food or seasoning, and can also be used as medicinal materials. Garlic is rich in nutrients. Every 100 grams contains 69.8 grams of water, 4.4 grams of protein, 0.2 grams of fat, 23.6 grams of carbohydrates, 5 mg of calcium, 44 mg of phosphorus, 0.4 mg of iron, and 3 mg of vitamin C. In addition, it also contains thiamine, riboflavin, niacin, allicin, citral, and trace elements such as selenium and germanium. Contains about 0.2% volatile oil. The main ingredient in the oil is allicin, which has a bactericidal effect. It is produced by the hydrolysis of alliin contained in garlic by t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李本明
Owner 重庆市黔江区阿蓬科技企业孵化器有限公司
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