Peony standardized quick-breeding method

A peony, fast technology, applied in the field of botany, can solve the problems of asexual reproduction, the influence of breeding and promotion of famous and excellent peony varieties, and poor seed development.

Inactive Publication Date: 2006-05-10
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to long-term artificial cultivation, most of the famous and high-quality varieties of peony have reduced their seed-setting ability, and their seeds are poorly developed, so they can only reproduce asexually.

Method used

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  • Peony standardized quick-breeding method

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[0018] specific implementation plan

[0019] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In this application, unless otherwise stated, the units of all reagents are expressed in mg / L.

[0020] Materials and methods:

[0021] Test materials: Peony (Paeonia suffruticosa) famous and valuable varieties are "Qinglongwomochi"; "No. 18 (Ruihong)"; "Yaohuang"; "Weizi"; "; "Hu Hong"; "Zhao Fen"; "Carmine";

[0022] 1) Culture of axillary buds

[0023] Take the axillary buds of the above-mentioned 10 varieties of plants in the first and middle days of March, peel off the 4-6mm long stem tips from the axillary buds, soak them in 75% alcohol for 30 seconds, and then soak them in 5% sodium hypochlorite solution for 8-10 minutes. Sterilize the surf...

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Abstract

The invention discloses a method of fast breeding peony, comprising: a) getting axillary bud bulk tip or tender leaf stalk from plants and culturing them in the culture medium until the callus formation and germination, b) cutting off the clump bud from the callus and culturing them until the bud grows to a height of 3-5 cm; c) inserting the bud in step b) into solutions containing indolebutyric acid, then culturing in the MS solid culture medium added with indolebutyric acid, benzyl amino adenine, active carbon, saccharose and macronutrient whose concentration reduced halves to form small plants; d) replanting the small plants. The invention develops a method of mass fast breeding peony by employing technique of plant cell tissue and forms a mass production process which produces tube sprout of peony ten thousand plants per year.

Description

technical field [0001] The present invention relates to the field of botany. More preferably, the present invention relates to a method for large-scale rapid propagation of peony. Background technique [0002] Peony (Paeonia suffruticosa) is a candidate national flower of our country. It is known as the beauty of the country and the king of flowers. It originated in my country and has evolved into four major populations. Domestic use of high-tech has also made great progress in the breeding of new varieties of peony and the regulation of flowering period by temperature control and chemical control. However, due to long-term artificial cultivation, most of the famous peony varieties have reduced fruiting ability and poor seed development, so they can only reproduce asexually. When cultivating varieties with distant hybrids, they often encounter problems such as hybrid sterility or poor seed development and failure to germinate. . These factors have an impact on the breedin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 卫志明
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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