Agrobacterium-mediated mature maize embryo shoot apex transformation method
An Agrobacterium-mediated, mature embryo technology, applied in biochemical equipment and methods, botanical equipment and methods, angiosperms/flowering plants, etc. question
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[0089] 1. Maize stem tip culture and carrier construction:
[0090] 1. Corn stem tip culture
[0091] 1.1 Select plump and undamaged seeds, put them into a triangular flask and carry out two sterilization treatments:
[0092] The first pass: Sterilize with 70% ethanol for 8 minutes, sterilize with 0.1% mercuric chloride aqueous solution for six minutes, wash with sterile water three times: wash with sterile water for 2 times the seed volume for one minute for the first time, and 3 times for the second time Wash the seed volume with sterile water for 2-3 minutes, wash with sterile water of 2 times the seed volume for one minute for the third time, then add 1.5 times the seed volume of sterile water to soak the seeds for 6 hours (24-26 degrees Celsius, if the temperature is too high, The time can be shortened appropriately), the time of soaking must be well grasped, if the time is too long, the seeds will easily turn black, and the rate of germination will decrease;
[0093] T...
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