Method for preparing rebaudioside by use of stevioside

A technology of steviolbioside and stevioside, which is applied in the direction of fixing on/in organic carrier, fermentation, etc., can solve the problems of short service life and long reaction time of β-glucosidase, and achieve high economic value and high Selectivity, effect of improving thermal stability
CN102925518AInactive Publication Date: 2013-02-13JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2013-02-13
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for preparing rebaudioside through packed bed enzymatic hydrolysis of stevioside. The method comprises the following steps of: dissolving the stevioside or stevia rebaudiana extract in water or phosphate buffer solution; combining the parts containing rebaudioside of the solution through an immobilization beta-glucosidase packed column; and concentrating and recrystallizing to obtain the rebaudioside. The preparation of rebaudioside takes 5 days in the prior art, and the transformation activity of the beta-glucosidase repeatedly used for 4 times is only 57.72% of the original activity. Through the invention, the preparation of rebaudioside using the immobilization beta-glucosidase only takes 12 hours, and the transformation rate of stevioside after 5 times of use still exceeds 84%. Thus, the method provided by the invention is short in production period, high in production efficiency and easy to realize continuous production, and has very broad application prospects.
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Description

【Technical field】

[0001] The invention belongs to the technical field of organic compound biosynthesis. More specifically, the present invention relates to a method for preparing steviolbioside by using a packed bed to enzymatically hydrolyze stevioside. 【Background technique】

[0002] Stevioside is a mixture of various diterpene glycosides extracted from the leaves of Stevia rebaudiana. It is a natural and powerful sweetener. A, RA), in which rebaudioside A has a pure sweet taste, while stevioside has a certain after-bitter taste. At present, a large number of studies mainly use enzymatic transglycosylation reaction to change the structure of stevioside to improve its sweetness characteristics. In addition, studies have shown that enzymatic hydrolysis can convert stevioside into stevioside with poor sweetness but certain physiological activity, and stevioside can be further derivatized as an intermediate product into other substances with better physiological activity. ...

Claims

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