Method for preparing high-color-value gardenia blue pigment

Abstract

The invention relates to a method for preparing a high-color-value gardenia blue pigment. The invention aims to provide a method for preparing the high-color-value gardenia blue pigment through fully utilizing plenty of gardenoside in gardenia yellow waste liquor. The gardenia blue pigment prepared by adopting the method has the characteristics of high color value, high quality, high purity, environmental friendliness, and the like. The method for preparing the high-color-value gardenia blue pigment comprises the following steps of (1) gardenoside enzyme hydrolysis: carrying out enzyme hydrolysis on the gardenia yellow waste liquor with beta-glucosidase as the hydrolytic enzyme; (2) blue turning of the gardenoside hydrolysate: adding amino acid into the gardenoside hydrolysate obtained in the step (1) to react; (3) ultrafiltration separation of the gardenia blue pigment: carrying out ultrafiltration on the blued solution obtained in the step (2) by using an ultrafiltration membrane; and (4) extraction and purification of the gardenia blue pigment: drying trapped fluid obtained in the step (3) and carrying out extraction through mixing with methyl alcohol or ethyl alcohol to obtain the high-color-value gardenia blue pigment.

Description

Technical field

[0001] The invention relates to a method for preparing gardenia blue pigment with high color value. Background technique

[0002] Gardenia blue pigment is a water-soluble natural pigment, the aqueous solution is bright and pure blue, the dry powder is blue-black, non-toxic, no odor, easily soluble in water, strong coloring power, stable performance, my country has been in 1989 It was listed in the new varieties of food additives licensed for use in the year, and it is gradually replacing synthetic pigments indigo and brilliant blue, and is used in food, medicine and cosmetics industries. The preparation of gardenia blue pigment mostly adopts the conversion of gardenoside by microbial fermentation or β-glucosidase hydrolysis. The separation and purification methods of gardenia blue pigment mainly include macroporous adsorption resin method and membrane separation method. If only the macroporous adsorption resin method and membrane separation method are used, the o...