Alkaline pectic enzyme producing engineering strain and its construction and method for producing alkaline pectic enzyme with the same

A technology of pectinase and engineering bacteria, which is applied in the field of bioengineering, can solve the problems of lack of research reports on genetic engineering of alkaline pectin ester lyase, staying in the stage of strain screening and research on enzyme characteristics, and shortening the fermentation time. , less variety, the effect of simplifying the purification process
CN101157900AInactive Publication Date: 2008-04-09JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2008-04-09
Estimated Expiration
Not applicable · inactive patent
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Abstract

An engineering bacterium of producing alkaline pectinase, a construction thereof and a method of producing alkaline pectinase by using the alkaline pertain to the technical field of bioengineering. The invention uses nonpathogenic bacillus sp WSHB04-02 genes group as a model and adopts amplification technology of PCR. And then the sequence pel containing 1.2kb coding alkaline pectinase genes section is obtained. At the same time from the point of the demand for industrial production, the invention makes use of a shuttle expression carrier to restructure yeast Pichiapastoris(pel), which means to produce engineering bacterium CGMCC No.2143 of alkaline pectinase (E.C.4.2.2.2). The invention also provides CGMCC No.2143 as the method of producing alkaline pectinase for fermented yeast strain. The genes engineering bacterium aims at exocytosis of protein and the species of extracellular protein is few, which simplifies the purification technique of producing alkaline pectinase by using microorganism and reduces the cost and shoetens the time for ferment. Besides, the invention improves the production efficiency and lays a foundation for industrialization of the ferment method of producing alkaline pectinase by using microorganism.
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Description

technical field

[0001] An engineering bacterium producing alkaline pectinase, its construction and a method for producing alkaline pectinase with the bacterium belong to the technical field of bioengineering. Background technique

[0002] Pectinase refers to a type of enzyme that can decompose pectin, and alkaline pectinase (E.C.4.2.2.2) refers to a type of pectinase with relatively high activity in the alkaline range. In addition to the application of alkaline pectinase in the purification of plant viruses and pulp bleaching, it has become a vital biological enzyme preparation in the pretreatment of cotton fabrics. The use of alkaline pectinase will change the traditional alkali scouring process, not only reduce environmental pollution, save a lot of process water, but also improve the quality of cotton fabrics.

[0003] In the past ten years, foreign researchers have been trying to use genetic engineering to construct high-yield strains of pectin ester lyase. Alkaline pec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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