Method of using alpha-glucosidase for preparing isomaltooligosaccharide

Abstract

The invention relates to a method of using alpha-glucosidase for preparing isomaltooligosaccharide. The method includes alpha-glucosidase immobilization, continuous reaction and isomaltooligosaccharide preparation. The alpha-glucosidase is immobilized through alginate, carrageenan and chitosan to implement continuous reaction, converting rate of raw material is 75%, and production cost is reduced by 1/3. By the aid of the method, isomaltooligosaccharide 50 or total isomaltooligosaccharide content of the isomaltooligosaccharide 50 is 55%, and the total content of isomalt, panose and isomaltotriose is 35%; isomaltooligosaccharide 90 or total isomaltooligosaccharide content of the isomaltooligosaccharide 90 is 95%, and the the total content of isomalt, panose and isomaltotriose is 70%.

Description

【Technical field】 [0001] The invention belongs to the technical field of functional oligosaccharides. More specifically, the present invention relates to a method for preparing isomaltooligosaccharide using α-glucosidase. 【Background technique】 [0002] Oligosaccharide refers to a polymeric sugar composed of 2 to 10 monosaccharide molecules linked by glycosidic bonds. Its molecular weight is between 200 and 2000 Da. Due to the different binding positions and types of monosaccharide molecules, oligosaccharides have various types and functions. Oligosaccharides can be divided into common oligosaccharides and functional oligosaccharides according to their functions. Functional oligosaccharides refer to sugars that have special physiological effects on humans, animals, microorganisms, plants, etc., including isomaltose, stachyose, raffinose, isomaltulose, lactulose, xylem Sugar, galactooligosaccharide, lactulose oligosaccharide, sucrose oligosaccharide, palatinose oligosacch...

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Problems solved by technology

Existing patent applications for preparing isomaltooligosaccharides include, for example, CN 200610105282, CN 201010136714, CN 200410025748, CN 201210062892, CN 201110307159, CN 201210221599, CN 201210221599, CN 200910114522 and CN 201070. Enzyme reverse reaction, the enzyme reverses in a glucose solution with a concentration of up to 65%, and condenses glucose into oligosaccharide isomaltose, of which the content of isomaltooligosaccharide is only 20% to 30%, and the product composition is complex and has many impurities , long production cycle, not suitable for industrial production, such as CN 201210062892, CN 201110307159, etc.; the second type is to use α-glucosidase to transfer glucose to another carbohydrate substrate to form α-1,6 glycosidic bonds, thereby Forming isomaltooligosaccharide, the enzyme cannot be reused, continuous and automated, and the cost is high, such as CN 200410025748, CN 201220221599, etc.; the third category is CN 200910114522, CN 201220223072, although they involve immobilized cells and immobilized yeast The preparation of strains is collectively referred to as immobilized cell technology. Although it can be continuous and automated, it is not immobilized α-glucosidase technology.

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