Functional enhancement of microorganisms to minimize production of acrylamide

A microorganism and asparagine technology, which is applied to genetically modified microorganisms and food fields with reduced acrylamide content, can solve the problems of reducing the deep fermentation time of acrylamide and being impractical.

Active Publication Date: 2013-01-09
复兴生物科技公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this deep fermentation time, which is effective in reducing

Method used

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  • Functional enhancement of microorganisms to minimize production of acrylamide
  • Functional enhancement of microorganisms to minimize production of acrylamide
  • Functional enhancement of microorganisms to minimize production of acrylamide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Example 1: Cloning and constitutive expression of ASP3, ASP1, GAP1, GNP1, AGP1, AGP2, AGP3 and GAT1 genes and deletion of URE2, TOR1, DAL80, and GZF3 in Saccharomyces cerevisiae strains

[0104] For clonal selection, the antibiotic resistance marker kanMX was used. Transform industrial / commercial baker's yeast or experimental strains to constitutively express ASP3, ASP1, GAP1, GNP1, AGP1, AGP2, AGP3 or GAT1, or a combination of ASP3 and GAP1 or a combination of ASP3 and GAT1, or to delete URE2, TOR1 , DAL80 or GZF3 genes or a combination with tor1Δ and ASP3 overexpression. The only genetic and metabolic modifications are the expected constitutive expression of: ASP3, ASP1, GAP1, GNP1, AGP1, AGP2, AGP3, or GAT1, or a combination of ASP3 and GAP1 or a combination of ASP3 and GAT1, or deletion of URE2, TOR1, DAL80 and GZF3 genes or a combination with tor1Δ and ASP3 overexpression.

Embodiment 2

[0105] Example 2: Transformation of Yeast with ASP3, ASP1, GAP1, GNP1, AGP1, AGP2, AGP3 or GAT1 Gene Cassette or URE2 Deletion Gene Cassette

[0106] Yeast is transformed with a recombinant nucleic acid containing the ASP3, ASP1, GAP1, GNP1, AGP1, AGP2, AGP3 or GAT1 gene under the control of the PGK1 promoter and terminator signal. The PGK1 promoter is not affected by NCR. The URE2 deletion cassette contains 5' and 3' URE2 flanking sequences for target gene deletion.

Embodiment 3

[0107] Example 3: Self-cloning gene cassettes allowing removal of selectable markers

[0108] Figure 1-8 It is shown how the designed gene cassette allows selection of transformed yeast and subsequent removal of antibiotic resistance markers by direct repeats as used in the Examples described below. As shown in other examples, the ASP1 self-cloning gene cassette was constructed, transformed and antibiotic resistance marker removed in a similar manner.

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Abstract

The present disclosure provides yeast transformed with a nucleic acid molecule (GAT1 ) to reduce nitrogen catabolite repression of asparagine transport/degradation and/or overexpress genes (ASP1 or ASP3) encoding cell- wall or extracellular proteins involved in asparagine degradation and/or genes (AGP1 or GNP1 or GAP1 ) encoding proteins involved in asparagine transport under food preparation/processing conditions. The genetically modified yeast has enhanced ability to reduce acnlamide concentration in foods prepared by heating. Also provided are methods and uses of the transgenic yeast for reducing acnlamide in a food product and food products having reduced acrylamide content prepared using the transgenic yeast.

Description

[0001] related application [0002] This application claims priority based on corresponding U.S. Provisional Patent Application Nos. 61 / 309,623 and 61 / 316,634, filed March 2, 2010, and March 23, 2010, respectively, claiming the benefit of 35 U.S.C. 119, incorporated by reference All of them are incorporated herein. technical field [0003] The present disclosure relates to products and methods for reducing acrylamide in foods, and also to foods with reduced acrylamide content. In particular, the present disclosure relates to genetically engineered microorganisms to enhance their ability to reduce acrylamide. Background technique [0004] Acrylamide is a colorless and odorless crystalline solid, which is an important industrial monomer, often used as cement binder and for the synthesis of polymers and gels. Based on various in vivo and in vitro studies, the carcinogenic and genotoxic effects of acrylamide and its metabolite glycidamide have been clearly demonstrated (Wilson...

Claims

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Application Information

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IPC IPC(8): C12N1/21A23L1/00A23L1/30C12N1/15C12N1/19C12N15/00C12N15/55C12N15/63C12N15/81C12N9/82A23L5/20A23L11/00A23L13/00A23L19/00A23L19/18
CPCA23L1/0158C12N9/82C07K14/705C12N15/81C07K14/395A23L5/28
Inventor A·丘恩J·I·胡斯尼克
Owner 复兴生物科技公司
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