Method for crude extraction of periplaneta americana breeding waste and application of periplaneta americana breeding waste

Abstract

The invention discloses a method for crude extraction of periplaneta americana breeding waste and application of the periplaneta americana breeding waste, and belongs to the technical field of periplaneta americana breeding waste extraction. The method for coarsely extracting the American cockroach breeding waste comprises the following steps: (1) grinding the American cockroach breeding waste to obtain American cockroach breeding waste powder, weighing the American cockroach breeding waste powder, and putting the American cockroach breeding waste powder into a container; and (2) preparing a leaching solution, namely adding the American cockroach breeding waste powder into absolute ethyl alcohol, fully and uniformly mixing, sealing by using a preservative film, leaching and filtering at room temperature to obtain the leaching solution, and storing in a refrigerator. The American cockroach breeding waste excrement, shell and ootheca crude extract have different degrees of bacteriostatic activity on fusarium verticillium and alternaria alternata; the method has the advantages of small environmental pollution, difficult generation of drug resistance and the like, and can avoid the problems caused by the use of chemical bactericides; a new thought is provided for innocent treatment and recycling of the American cockroach breeding waste.

Description

Technical Field

[0001] The present invention relates to the technical field of extracting waste from Periplaneta americana breeding, and specifically relates to a method for roughly extracting waste from Periplaneta americana breeding and its application. Background Art

[0002] In agricultural production, plant diseases are regarded as a major limiting factor, which pose a serious threat to global agricultural production and food safety. Plant diseases can be mainly divided into two categories: non-infectious diseases and infectious diseases. Non-infectious diseases are mainly caused by abiotic factors and are reversible plant diseases that can be restored by improving environmental conditions in the early stage of disease onset. Infectious diseases, on the other hand, are irreversible diseases caused by various pathogens, including but not limited to bacteria, fungi, viruses, nematodes, and parasitic seed plants. Among them, fungal diseases caused by plant pathogenic fungi are widely spread and cause greater harm to plants. For example, Fusarium verticillioides can cause various diseases in corn, such as seedling blight, ear rot, stalk rot, and seed rot, which can lead to a large-scale reduction in corn production. Alternaria alternata can cause black spot disease in edible roses and vegetable crops. In the initial stage of infection, brown spots appear on the leaves, and then expand to form disease spots. As the disease progresses, the disease spot area will turn black and dry. When the disease is severe, a large number of leaves will fall off, the branches will become bare, the growth will weaken, and even the flowering of the plant will be affected, resulting in a decrease in yield. Although traditional chemical fungicides are the preferred method for controlling plant diseases, if a single chemical fungicide is used in large quantities for a long time, it may cause a series of problems such as environmental pollution, ecological damage, food residues, and pathogen resistance. Natural antibacterial agents extracted from natural resources such as plants and animals have the advantages of less environmental pollution and less tendency to produce drug resistance, and can avoid the problems caused by the use of chemical fungicides.

[0003] The American cockroach (Periplaneta americana L.), commonly known as the cockroach, is an insect of the genus Periplaneta in the order Blattodea, subclass Pterygota, class Insecta. According to modern pharmacological research, the American cockroach exhibits various biological activities, such as wound repair, antibacterial, antiviral, and antitumor effects. Some scholars have also found that endophytic Microbacterium, Mycobacterium, and Achromobacter isolated from the intestine of the American cockroach show obvious antibacterial activity. In addition, the brass extract of the American cockroach also shows significant antibacterial effects against Escherichia coli. The American cockroach exhibits strong antibacterial ability. Some studies have also pointed out that the main antibacterial substances in the American cockroach are single-component active polypeptides, such as periplanetasin-2 and periplanetasin-4. With the development of the medicinal value and antibacterial activity of the American cockroach, and the expansion of the artificial breeding range, the breeding conditions of the American cockroach and its influencing factors have been gradually improved, and the breeding of the American cockroach has gradually become professional. A large amount of breeding waste, such as feces, shells, and oothecae, is generated during the breeding process of the American cockroach. The harmless treatment and recycling of these wastes are also a major problem in the current breeding of the American cockroach. The present invention studies whether the crude extract of the breeding waste of the American cockroach has an inhibitory effect on plant pathogenic fungi and whether it can be used for the development of natural antibacterial agents. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for crude extraction of the breeding waste of the American cockroach and its application, which can inhibit Fusarium verticillioides and Alternaria alternata, and solve a series of problems such as environmental pollution, ecological damage, food residues, and pathogen resistance.

[0005] To achieve the above object, the present invention provides the following technical solution: A method for crude extraction of the breeding waste of the American cockroach, and the method for crude extraction of the breeding waste of the American cockroach is as follows:

[0006] S1: Grind the breeding waste of the American cockroach

[0007] Grind the breeding waste of the American cockroach to obtain the powder of the breeding waste of the American cockroach, and weigh the powder of the breeding waste of the American cockroach and place it in a container;

[0008] S2: Prepare the extract

[0009] Add the powder of the breeding waste of the American cockroach to absolute ethanol, mix well, seal it with plastic wrap, and place it at room temperature for extraction and filtration to obtain the extract, which is stored in the refrigerator.

[0010] Preferably, in the step S1, the powder of the breeding waste of the American cockroach is obtained after passing through a 20-mesh sieve.

[0011] Preferably, in the step S1, the breeding waste of the American cockroach is the feces, shells, and oothecae of the breeding waste of the American cockroach.

[0012] Preferably, in the step S2, the ratio of the Periplaneta americana breeding waste powder to the absolute ethanol is 1:3 (g / ml).

[0013] Preferably, in the step S2, the extraction of the extract is specifically carried out by sealing the container containing the Periplaneta americana breeding waste powder and the absolute ethanol with a plastic wrap and then placing it at room temperature of 25 °C for 24 h for extraction, and then filtering to obtain the extract.

[0014] Preferably, the condition for refrigerator storage is that the extract is stored in a 4 °C refrigerator.

[0015] The crudely extracted Periplaneta americana breeding waste obtained by the method for crudely extracting Periplaneta americana breeding waste is applied to inhibiting the activity of Fusarium verticillioides.

[0016] The crudely extracted Periplaneta americana breeding waste obtained by the method for crudely extracting Periplaneta americana breeding waste is applied to inhibiting the activity of Alternaria alternata.

[0017] The crudely extracted Periplaneta americana breeding waste obtained by the method for crudely extracting Periplaneta americana breeding waste is applied to inhibiting the activity of black spot disease of edible roses.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0019] The crude extracts of Periplaneta americana breeding waste feces, shells and egg cases of the present invention all have different degrees of antibacterial activities against Fusarium verticillioides and Alternaria alternata. Among them, the crude extracts of Periplaneta americana shells and egg cases have the best antibacterial effects against Fusarium verticillioides; the crude extracts of Periplaneta americana shells and egg cases have the best antibacterial effects against Alternaria alternata. The present invention has the advantages of small environmental pollution and low resistance generation in inhibiting Fusarium verticillioides and Alternaria alternata, and can avoid the problems brought by the use of chemical fungicides. The present invention provides a scientific basis for the development of new natural antibacterial agents and also provides a new idea for the harmless treatment and recycling of Periplaneta americana breeding waste. Description of the Drawings

[0020] Figure 1 Colony diameters of Fusarium verticillioides under treatment with crude extracts of Periplaneta americana breeding waste at different concentrations.

[0021] [[ID=3able]] Figure 2 Inhibition rates of crude extracts of Periplaneta americana breeding waste at different concentrations against Fusarium verticillioides;

[0022] Figure 3 Inhibiting effects of crude extracts of Periplaneta americana breeding waste at different concentrations against Fusarium verticillioides;

[0023] Figure 4Colony diameter of Alternaria alternata under the treatment of Periplaneta americana breeding waste at different concentrations;

[0024] Figure 5 Inhibitory rate of Periplaneta americana breeding waste at different concentrations on Alternaria alternata;

[0025] Figure 6 Inhibitory effect of crude extracts of Periplaneta americana breeding waste at different concentrations on Alternaria alternata spores;

[0026] Figure 7 Graph showing the change of the disease index of Alternaria alternata on edible roses over time in each treatment group;

[0027] Figure 8 Photos of the field changes of Alternaria alternata on edible roses. Detailed implementation method

[0028] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without any creative work belong to the scope of protection of the present invention. The experimental methods described in the following embodiments are all conventional methods unless otherwise specified; the reagents and materials are all commercially available unless otherwise specified.

[0029] The present invention takes Periplaneta americana breeding waste (feces, shells, and oothecae) as the research object, prepares its absolute ethanol crude extract by the maceration method, and uses the mycelial growth rate method to measure the inhibitory effects of three crude extracts at different concentrations on Alternaria alternata and Fusarium verticillioides, as well as the field control effect of the crude extracts of shells and oothecae on Alternaria alternata of edible roses, providing ideas for the development of new natural bacteriostatic agents and the scientific utilization of Periplaneta americana breeding waste.

[0030] Embodiment

[0031] (1) Test materials

[0032] Materials: Periplaneta americana breeding waste (feces, shells, oothecae); Test plant pathogenic fungi: Alternaria alternata, Fusarium verticillioides; Medium: Potato dextrose agar (PDA) medium.

[0033] (2) Obtaining crude extracts of Periplaneta americana breeding waste

[0034] Using the direct immersion extraction method, 100 g of the breeding waste of Periplaneta americana (feces, shells, oothecae) after passing through a 20-mesh sieve was weighed and placed in a 500-ml conical flask. 300 ml of absolute ethanol was added according to the solid-liquid ratio of 1:3 (g / ml). After thorough mixing, the flask was sealed with plastic wrap and immersed at room temperature of 25°C for 24 h. Then, the extract was filtered to obtain the extraction solution, which was stored in a refrigerator at 4°C.

[0035] (3) Antibacterial test

[0036] The mycelial growth rate method was used in the test. Under aseptic conditions, the crude extracts of the breeding waste of Periplaneta americana (feces, shells, oothecae) were filtered through a bacterial filter and then added to PDA medium to prepare 4 kinds of PDA media containing the crude extracts of Periplaneta americana feces, oothecae and shells at concentrations of 10 ml / L, 20 ml / L, 30 ml / L, and 40 ml / L. The medium containing the extraction solvent absolute ethanol was used as the blank control. An 8-mm test plant pathogenic fungal disc was inoculated at the center of each plate, and 6 replicates were set for each concentration.

[0037] The inoculated plates were placed in an incubator at 28°C. When the colony diameter of the control group grew to 2 / 3 of the petri dish, the colony diameter of the test fungus in each plate at each concentration was measured using the cross method, and the average value of 6 replicate tests was calculated. The calculation formula of the inhibition rate is shown in Equation 1.

[0038]

[0039] Based on the concentration and inhibition rate data, significant difference analysis was performed using SPSS 27.01 software, the toxicity regression equation and correlation coefficient were obtained using Excel software, and the median lethal concentration value (EC50) was calculated.

[0040] (4) Field control effect test

[0041] During the above antibacterial test, it was found that the antibacterial effects of the crude extracts of Periplaneta americana shells and oothecae on Alternaria alternata at a concentration of 40 ml / L were more obvious and significantly higher than those of the feces crude extract. Therefore, only the crude extracts of Periplaneta americana shells and oothecae and the water control were used in the field control effect test of rose black spot, and the feces crude extract was not used. Alternaria alternata can cause rose black spot. It was found that rose black spot was relatively serious in the practice teaching base of Dali University. This test was carried out in the rose field of the practice teaching base of a university in October 2024.

[0042] Test design: The test plot was randomly divided into 5 sub-plots, with 20 plants in each sub-plot. Different treatments were used for each sub-plot as shown in Table 1. The medicine was applied 4 times. The number of diseased leaves at each level was investigated before each application of the medicine, and the disease condition and control effect were calculated according to Equations (2) and (3).

[0043] Table 1 Field control effect treatment methods of black spot disease of edible roses

[0044]

[0045]

[0046]

[0047] Field control effect investigation: The investigation method and disease grading standard refer to Table 2 of the "Pesticide Field Efficacy Test Guidelines (Part 2)". Calculate the control effect and conduct significance determination. Randomly investigate 4 edible roses in each plot, and investigate the disease occurrence of all leaves of each plant.

[0048] Table 2 Black spot disease grading standard and representative values of each level

[0049]

[0050] Application example effect and analysis

[0051] (1) Antibacterial activity of crude extract from Periplaneta americana breeding waste against Fusarium verticillioides

[0052] It can be seen from Figure 1 that as the concentration of the crude extract from Periplaneta americana breeding waste (feces, shells, oothecae) increases, the degree of inhibition of mycelial growth increases, and the colony growth diameter of Fusarium verticillioides generally shows a downward trend, and there is a significant difference from the control group. The colony growth diameter of the feces crude extract treatment group is significantly higher than that of the shell crude extract treatment group and the ootheca crude extract treatment group. It can be seen from Figure 2 that the crude extracts from 3 kinds of Periplaneta americana breeding waste (feces, shells, oothecae) all have different degrees of inhibitory effects on Fusarium verticillioides. The higher the concentration of the crude extract, the more obvious the inhibitory effect. The best inhibitory effects are the shell crude extract and the ootheca crude extract, and the highest inhibition rates reach 85.26% and 86.3% respectively, which are significantly higher than 69.60% of the feces crude extract. At lower concentrations (10 ml / L and 20 ml / L) of treatment, the inhibition rates of the feces crude extract and the ootheca crude extract on Fusarium verticillioides are significantly higher than that of the shell crude extract; at higher concentrations (30 ml / L and 40 ml / L) of treatment, the inhibition rates of the Periplaneta americana shell crude extract and the ootheca crude extract on Fusarium verticillioides are significantly higher than that of the feces crude extract (P < 0.05). Figure 1 and Figure 2 Different lowercase letters in Figure 3 represent P < 0.05.

[0053] shows the inhibitory effects of crude extracts from different concentrations of Periplaneta americana breeding waste on Fusarium verticillioides. (a) Each treatment group of feces crude extract, (b) each treatment group of shell crude extract, (c) each treatment group of ootheca crude extract.

[0053] The crude extracts of three kinds of Periplaneta americana breeding wastes (feces, shells, oothecae) all have good inhibitory effects on Periplaneta americana, and the higher the concentration of the crude extract of Periplaneta americana breeding waste, the more obvious the inhibitory effect on Fusarium verticillioides, and the lower the concentration, the smaller the inhibitory effect. As can be seen from Table 3, the EC50 values of the crude extracts of the three kinds of Periplaneta americana breeding wastes are 25.8647 ml / L, 23.3239 ml / L and 22.4670 ml / L respectively, and the antibacterial effects on Fusarium verticillioides from large to small are ootheca > shell > feces.

[0054] Table 3 Toxicity regression equation of the crude extract of Periplaneta americana breeding waste to Fusarium verticillioides

[0055]

[0056] (2) Antibacterial activity of the crude extract of Periplaneta americana breeding waste against Alternaria alternata

[0057] As Figure 4 can be seen, with the increase of the concentration of the crude extract of Periplaneta americana breeding waste (feces, shells, oothecae), the inhibitory effect on the mycelial growth of Alternaria alternata is enhanced, the colony growth diameter generally tends to decrease, and there is a significant difference from the control group. The colony growth diameter of the feces crude extract treatment group is significantly higher than that of the shell crude extract treatment group and the ootheca crude extract treatment group. As Figure 5 can be seen, the crude extracts of different Periplaneta americana breeding wastes (feces, shells, oothecae) all have different degrees of inhibitory effects on Alternaria alternata. The higher the concentration of the crude extract, the more obvious the inhibitory effect. The best inhibitory effects are the shell crude extract and the ootheca crude extract, and the highest inhibition rate reaches 100%, which is significantly higher than 67.91% of the feces crude extract. At a lower concentration (10 ml / L) treatment, the inhibitory rate of the ootheca crude extract on Alternaria alternata is better, which is significantly higher than that of the feces crude extract and the shell crude extract; when the concentration of the crude extract is 20 ml / L, 30 ml / L and 40 ml / L, the inhibitory rates of the shell crude extract and the ootheca crude extract on Alternaria alternata are significantly higher than that of the feces crude extract (P < 0.05). Figure 4 and Figure 5 In, different lowercase letters indicate P < 0.05. Figure 6 are the inhibitory effects of the crude extracts of different concentrations of Periplaneta americana breeding waste on Alternaria alternata spores. (a) Each treatment group of feces crude extract, (b) each treatment group of shell crude extract, (c) each treatment group of ootheca crude extract.

[0058] The crude extracts of three kinds of Periplaneta americana breeding wastes (feces, shells, oothecae) all have good inhibitory effects on Alternaria alternata. And the higher the concentration of the crude extract of Periplaneta americana breeding waste, the more obvious the inhibitory effect on Alternaria alternata, and the lower the concentration, the smaller the inhibitory effect. As can be seen from Table 4, the EC50 values of the crude extracts of the three breeding wastes against Alternaria alternata are 29.3832 ml / L, 19.2792 ml / L, and 18.8220 ml / L respectively, and the antibacterial effects from large to small are ootheca > shell > feces.

[0059] Table 4 Toxicity regression equation of crude extracts of Periplaneta americana breeding waste against Alternaria alternata

[0060]

[0061] (3) Field control effect of crude extract of Periplaneta americana breeding waste on black spot of edible rose

[0062] During the above antibacterial test, it was found that the antibacterial effects of the crude extracts of Periplaneta americana shells and oothecae on Alternaria alternata were obvious at a concentration of 40 ml / L, and significantly higher than that of the feces crude extract. Therefore, only the crude extracts of Periplaneta americana shells and oothecae and the water control were used in the field control effect test of black spot of edible rose, and the feces crude extract was not used. Figure 7 It is a graph showing the change of the disease index of black spot of edible rose in each treatment group over time. (a) Each treatment group was sprayed every 3 days, (b) Each treatment group was sprayed every 7 days. Figure 7 As can be seen, the disease index of black spot of edible rose in all treatment groups including the control group increased significantly after the first spraying whether sprayed every 3 days or every 7 days, but the disease index in the shell and ootheca treatment groups was lower than that in the control group. After the second spraying, the disease index of each treatment group decreased to varying degrees except for the blank control whether sprayed every 3 days or every 7 days, and the disease index in the crude extract treatment group of Periplaneta americana ootheca was lower than that in the shell crude extract treatment group. The disease index of black spot of edible rose in each treatment group sprayed every 7 days was lower than that in the treatment group sprayed every 3 days except for the blank control after the second spraying.

[0063] As can be seen from Table 5, the relative control effects of the shell crude extract and the ootheca crude extract on black spot of edible rose were 2.03% and 3.1% respectively 3 days after the first spraying, and the control effects were not obvious. With the increase of the spraying times, the relative control effects of the two crude extracts on black spot of edible rose increased to varying degrees. After the fourth spraying, the relative control effects of the two crude extracts on black spot of edible rose reached 38.68% and 43.09% respectively. Generally speaking, there are certain differences in the relative control effects of the shell crude extract and the ootheca crude extract on black spot of edible rose, and the control effect of the ootheca crude extract on black spot of edible rose is slightly better than that of the shell crude extract.

[0064] Table 5 Control effect of different crude extracts sprayed every 3 days on black spot of edible rose

[0065]

[0066] As can be seen from Table 6, the relative control effects of the crude extract of the shell and the crude extract of the ootheca on black spot of edible roses were 8.88% and 11.06% respectively 7 days after the first application. The control had a certain effect but was not obvious. With the increase in the number of applications, the relative control effects of the two crude extracts on black spot of edible roses increased to varying degrees. After the fourth application, the relative control effects of the crude extract of the shell and the crude extract of the ootheca on black spot of edible roses reached 52.29% and 56.32% respectively. Overall, there were certain differences in the relative control effects of the crude extract of the shell and the crude extract of the ootheca on black spot of edible roses. The overall control effect of the crude extract of the ootheca on black spot of edible roses was slightly better than that of the crude extract of the shell.

[0067] Table 6 Control effects of different crude extracts on black spot of edible roses when applied every 7 days

[0068]

[0069] Figure 8 These are the photos of the field changes of black spot of edible roses. (a), (b), and (c) are the photos of black spot of edible roses before application. (d), (e), and (f) are the photos on the 14th day after application of the control group, the treatment group with the crude extract of the shell, and the treatment group with the crude extract of the ootheca respectively. (h), (h), and (i) are the photos on the 28th day after application of the control group, the treatment group with the crude extract of the shell, and the treatment group with the crude extract of the ootheca respectively.

[0070] The test results of applying the crude extracts of two Periplaneta americana breeding wastes (shell and ootheca) every three days and every seven days on black spot of edible roses were as follows: The control effect of applying the crude extract of the ootheca every seven days was the best, and its relative control effect reached 56.32% (28d) after 4 applications. Overall, the control effect of applying the crude extracts of the shell and the ootheca every seven days on black spot of edible roses was better than that of applying them every three days. The overall control effect of the crude extract of the ootheca was slightly better than that of the crude extract of the shell. The above antibacterial test results also showed that the antibacterial effect of the crude extract of the ootheca was better than that of the crude extract of the shell. The research results of the field control effect of 5 fungicides on black spot of Chrysanthemum morifolium by Tang Shiyong et al. showed that the field control effect of applying fluosilazole every seven days on black spot was the best, and the control effect reached 70% 28 days after the first application. The better control effect of applying the shell and ootheca of Periplaneta americana every seven days may be because the application interval of every seven days is longer, the growth period of the leaves of edible roses is long, the recovery time of diseased leaves is sufficient, and the leaves with higher disease levels fall off, thus affecting the overall disease index, showing a better control effect of applying every seven days than applying every three days.

[0071] In addition, it was observed in the experiment that when the disease severity of the diseased leaves of Rosa rugosa reached level 3 or above, since the representative value of the disease severity above level 3 was relatively large, this might lead to an upward trend in the calculated disease index of Rosa rugosa black spot disease for some time after pesticide application, which could make people mistakenly believe that the control effect of the pesticide was not good. The field control effect test results of a new fungicide and its combination on pear black spot disease by Hu Changxiao et al. also showed that there was a certain upward trend in the disease index after the first pesticide application, but it was lower than that of the control group. It was observed in the experiment that the development of the disease was basically controlled after the second application of the two crude extracts of Periplaneta americana waste shells and oothecae, whether applied every three days or every seven days, and the control effect on Rosa rugosa black spot disease was relatively ideal. Therefore, the prevention and treatment effects of Rosa rugosa black spot disease are closely related to the control timing. The earlier the prevention and treatment of the disease, the easier it is for the leaves with less severe disease to recover to a healthy state after treatment with pesticides.

[0072] In summary, both the crude extract of Periplaneta americana shells and the crude extract of oothecae have a certain control effect on Rosa rugosa black spot disease. By comparing the control effects of applying the two crude extracts of Periplaneta americana waste every 3 days and every 7 days on Rosa rugosa black spot disease, it was found that the control effect of applying every 7 days was better than that of applying every 3 days.

[0073] The present invention uses the maceration extraction method to prepare the crude ethanol extract of the waste from Periplaneta americana breeding. The inhibitory effects of the crude extracts of the feces, shells, and egg cases of the waste from Periplaneta americana breeding on two plant pathogens, Fusarium verticillioides and Alternaria alternata, are compared by the mycelial growth rate method. The results show that the inhibitory effects of the three crude extracts of the waste from Periplaneta americana breeding on the two plant pathogens are enhanced with the increase in the concentration of the crude extract. When the mass concentration of the ethanol extract is 40 ml / L, the crude extracts of the feces, shells, and egg cases of the waste from Periplaneta americana breeding all have good inhibitory effects on Fusarium verticillioides, and the inhibition rates are 69.6% ± 1.25%, 85.26% ± 0.93%, and 86.35% ± 1.62% respectively. Some scholars used the mycelial growth rate method to screen out three compounds with stable chemical structures from 62 naphthenic sulfonamide compounds, which have inhibitory effects on the mycelial growth of Fusarium verticillioides, and the inhibitory effects also increase with the increase in concentration. When the concentration of the crude extracts of the feces, shells, and egg cases of the waste from Periplaneta americana breeding is 40 ml / L (4%), they also have good inhibitory effects on Alternaria alternata, and the inhibition rates are 67.91% ± 1.12%, 100%, and 100% respectively. The results of the inhibitory effect of the flavonoid extract of Sophora flavescens on Alternaria alternata by Wang Caiyun et al. using the mycelial growth rate method show that when the concentration of the total flavonoid solution of Sophora flavescens reaches 10%, the inhibitory effect on Alternaria alternata is the best, reaching 98.8%, indicating that the crude extract of the waste from Periplaneta americana breeding has a good inhibitory effect on Alternaria alternata. The above results all show that the waste from Periplaneta americana breeding (feces, shells, egg cases) all have good antibacterial effects, and many studies have shown that Periplaneta americana contains rich proteins, various amino acids, antibacterial peptides, lysozyme, and antibiotics, etc., which have the effects of inhibiting bacterial reproduction, antifungal, and antiviral. Among them, the antibacterial peptide of Periplaneta americana has different degrees of inhibitory effects on Escherichia coli, Staphylococcus aureus, and Salmonella; there are also studies showing that the fat-soluble chemical component monoglyceride in Periplaneta americana has strong antibacterial effects on Gram-positive bacteria, especially prominent antibacterial effects on Bacillus subtilis and Micrococcus luteus; some scholars have also isolated fungi and actinomycetes with antibacterial activities from the feces of Periplaneta americana, all indicating that Periplaneta americana and its feces both contain rich antibacterial resources, and their antibacterial components and antibacterial mechanisms remain to be explored.

[0074] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for roughly extracting the breeding waste of Periplaneta americana, characterized in that: The method for roughly extracting Periplaneta americana breeding waste is as follows: S1: Grind the Periplaneta americana breeding waste Grind the Periplaneta americana breeding waste to obtain Periplaneta americana breeding waste powder, and weigh the Periplaneta americana breeding waste powder and place it in a container; S2: Prepare the extraction solution Add the Periplaneta americana breeding waste powder to absolute ethanol, mix well, seal it with plastic wrap, and place it at room temperature for extraction and filtration to obtain the extraction solution, which is stored in the refrigerator.

2. The method for roughly extracting Periplaneta americana breeding waste according to claim 1, characterized in that: In the step S1, the Periplaneta americana breeding waste powder is obtained after passing through a 20-mesh sieve.

3. The method for roughly extracting the breeding waste of Periplaneta americana according to claim 1, characterized in that: In the step S1, the Periplaneta americana breeding waste is the feces, shells, and oothecae of the Periplaneta americana breeding waste.

4. A method for roughly extracting Periplaneta americana breeding waste according to claim 1, characterized in that: In the step S2, the ratio of the Periplaneta americana breeding waste powder to the absolute ethanol is 1:3 (g / ml).

5. A method for roughly extracting Periplaneta americana breeding waste according to claim 1, characterized in that: In the step S2, the extraction of the extraction solution is specifically carried out by sealing the container containing the Periplaneta americana breeding waste powder and the absolute ethanol with plastic wrap and placing it at 25°C at room temperature for 24 hours for extraction and filtration to obtain the extraction solution.

6. The method for roughly extracting Periplaneta americana breeding waste according to claim 1, characterized in that: The condition for storing in the refrigerator is that the extraction solution is stored in a 4°C refrigerator.

7. The roughly extracted Periplaneta americana breeding waste obtained by the method for roughly extracting Periplaneta americana breeding waste as described in claim 1 is applied to inhibit the activity of Fusarium verticillioides.

8. The roughly extracted Periplaneta americana breeding waste obtained by the method for roughly extracting Periplaneta americana breeding waste as described in claim 1 is applied to inhibit the activity of Alternaria alternata.

9. The roughly extracted Periplaneta americana breeding waste obtained by the method for roughly extracting Periplaneta americana breeding waste as described in claim 1 is applied to inhibit the activity of black spot disease of edible roses.

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