Anti-allergic special medical infant milk protein partial hydrolysis formula food composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of food engineering, and particularly relates to an anti-allergic special medical infant milk protein partial hydrolysis formula food composition, a preparation method and application. According to the infant milk protein partial hydrolysis formula food composition for the special medical purpose, the mass ratio of the 2 '-fucosyllactose to the lactobacillus acidophilus NCFM to the bifidobacterium animalis Bi-07 is (150-200): (5-10): (2-8). The partially hydrolyzed formula food composition provided by the invention can prevent respiratory tract infection and maintain the natural defense capability of children; the infant health is promoted; the method helps infants to establish healthy intestinal microflora, which is crucial to immune system and cognitive development.

Description

Technical Field

[0001] The invention belongs to the technical field of food engineering, and in particular relates to an anti-allergic special medical infant milk protein partially hydrolyzed formula food composition, a preparation method and an application. Background Art

[0002] Partially hydrolyzed protein (pHF) is a protein or small molecule peptide that is broken down into smaller fragments through certain biotechnology, thereby reducing the amount of antigenic active substances, reducing or eliminating the allergenicity of milk protein, and reducing the risk of milk protein allergy. Compared with deep hydrolysis products, partially hydrolyzed protein contains more allergens that can initiate allergic reactions, can induce oral tolerance to intact proteins, and is a good choice for preventing allergies in high-risk infants.

[0003] Infant feeding guidelines in Europe and the United States support the use of hydrolyzed formula instead of standard cow's milk formula if the infant is not breastfed to prevent allergic diseases in high-risk children, although there is still debate about whether there is enough evidence to support this recommendation. However, a systematic review showed that the use of pHF (100% whey) can significantly reduce the incidence of allergic diseases (especially atopic dermatitis or eczema) in high-risk children compared with the use of cow's milk protein formula. Compared with healthy infants, the number of bifidobacteria and lactobacilli in the intestines of allergic children is reduced, and Escherichia coli and Staphylococcus aureus are significantly increased. The proportion of Bacteroidetes is lower and the proportion of Enterobacter is higher.

[0004] For example, Chinese patent CN114176219A provides a low-allergenic special medical infant milk protein partially hydrolyzed formula food suitable for infants at high risk of milk protein allergy, characterized in that the formula food is mainly composed of the following components by weight: 1627-770 parts of vegetable fat powder, 2216-251 parts of vegetable fat powder, 18-22 parts of oligofructose, 8.8-13.2 parts of compound choline chloride, 5.6-8.4 parts of compound potassium chloride, 4-6 parts of compound calcium hydrogen phosphate, 4-6 parts of compound minerals, 2.8-4.2 parts of compound inositol, 2.4-4.6 parts of tricalcium phosphate, 2.7-4.1 parts of compound vitamins, 1.6-2.4 parts of compound sodium chloride, 0.8-1.2 parts of calcium carbonate, 0.4-0.6 parts of compound carnitine, and 0.4-0.6 parts of compound nucleotides. However, this invention cannot effectively regulate intestinal flora.

[0005] As Qi Xiaoyan et al. mentioned in "Research Progress and Market Development Application of Hypoallergenic Hydrolyzed Formula Milk Powder", due to the differences in the nutritional structure between bovine whey protein and breast milk, infants are prone to allergic reactions when consuming cow's milk before the age of one, including dermatitis, eczema, abdominal pain, diarrhea, constipation, asthma, etc. Preventing and promptly relieving allergic symptoms play an important role in the healthy development of infants and young children. This paper comprehensively discussed the current domestic and foreign research on hydrolyzed whey protein, respectively explored the importance of developing hydrolyzed protein formula milk powder, listed the current functional characteristics of hydrolyzed protein in infant anti-allergy, and compared the market application of hydrolyzed protein in infant formula powder, so as to research and develop a high-quality infant hydrolyzed formula milk powder with low allergenicity, easy absorption, promoting tolerance, and beneficial to the intestine. However, the invention did not conduct relevant experimental explorations to verify the effects.

[0006] Therefore, there is an urgent need to study a food composition that can regulate the intestinal flora, establish an intestinal barrier, and has anti-allergic properties. Summary of the Invention

[0007] Based on the deficiencies of the prior art, the present invention aims to provide a partially hydrolyzed formula food composition with anti-allergic function, its preparation method and application. The food composition provided by the present invention can regulate the intestinal flora, establish an intestinal barrier, and has anti-allergic properties.

[0008] To achieve the above object, the present invention adopts the following technical solutions:

[0009] On the one hand, the present invention provides a partially hydrolyzed formula food composition with anti-allergic function. Calculated by 100 g, it includes the following raw materials: 44 - 50 g of lactose, 20 - 25 g of compound vegetable oil, 20 - 25 g of moderately hydrolyzed whey protein, 1 - 2 g of choline chloride, 1 - 2 g of calcium hydrogen phosphate, 0.5 - 1.5 g of 2'-fucosyllactose, 0.6 - 1 g of mono- and diglycerol fatty acid esters, 0.6 - 1 g of potassium chloride, 0.5 - 1 g of sodium starch octenyl succinate, 0.4 - 1 g of solid corn syrup, 0.4 - 1 g of tricalcium phosphate, 0.4 - 1 g of maltodextrin, 0.3 - 1 g of arachidonic acid oil, 0.2 - 0.5 g of magnesium sulfate, 0.2 - 0.5 g of docosahexaenoic acid oil, 0.1 - 0.2 g of calcium carbonate, 0.05 - 0.1 g of mixed nucleotides, 0.02 - 0.05 g of Lactobacillus acidophilus NCFM, 0.02 - 0.05 g of Bifidobacterium animalis subsp. lactis Bi-07, 0.05 - 0.1 g of compound vitamins, and 0.05 - 0.1 g of compound minerals.

[0010] Preferably, the mass ratio of the 2'-fucosyllactose, Lactobacillus acidophilus NCFM, and Bifidobacterium animalis subsp. lactis Bi-07 is 100 - 300:3 - 9:4 - 8.

[0011] More preferably, the mass ratio of the 2'-fucosyllactose, Lactobacillus acidophilus NCFM, and Bifidobacterium animalis subsp. lactis Bi-07 is 170-190:7-8:5-7.

[0012] Even more preferably, the mass ratio of the 2'-fucosyllactose, Lactobacillus acidophilus NCFM, and Bifidobacterium animalis subsp. lactis Bi-07 is 180:7.5:5.

[0013] As a preferred embodiment, the partially hydrolyzed formula food composition, per 100 g, comprises the following raw materials:

[0014]

[0015]

[0016] In a second aspect, the present invention also provides a method for preparing the above-mentioned partially hydrolyzed formula food composition having an anti-allergy function, comprising the following steps: Mix according to the ratio.

[0017] In a third aspect, the present invention also provides the use of the above-mentioned partially hydrolyzed formula food composition having an anti-allergy function in the preparation of anti-allergy products.

[0018] Specifically, the products include foods, health products, or drugs.

[0019] Preferably, the food further comprises nutritionally acceptable nutritional additives.

[0020] Preferably, the nutritional additives include, but are not limited to, one or more of dietary fiber, prebiotics, proteins, lipid substances, minerals, and vitamins.

[0021] Preferably, the foods include tablet candies, soy milk, yogurt, canned foods, biscuits, chocolates, pastries, creams, cheeses, milk powders, formula milks, ice creams, jams, purees, candied fruits, preserved fruits, breads, egg rolls, protein drinks, solid drinks, lactic acid bacteria drinks, plant protein drinks, carbonated drinks, coffee, or puffed foods.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The present invention reduces the occurrence of allergic diseases by reducing the number of allergens and retains partial immunogenicity for oral immune tolerance.

[0024] (2) The present invention adds 2'-fucosyllactose, Lactobacillus acidophilus NCFM, and Bifidobacterium lactis Bi-07 to synergistically establish an intestinal barrier with 2'-fucosyllactose and stimulate the self-protection ability.

[0025] (3) The partially hydrolyzed formula food composition provided by the present invention can prevent respiratory tract infections and maintain the natural defense ability of children; it promotes the health of infants: helps infants establish a healthy gut microbiota, which is crucial for immune system and cognitive development. Detailed implementation manners

[0026] The technical solution of the present invention will be clearly and completely described below in specific implementation manners. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts belong to the scope of protection of the present invention. The materials, reagents, etc. used, unless otherwise specified, are reagents and materials that can be obtained commercially.

[0027] The purchase manufacturers and item numbers of the reagents used in the following examples are shown in Table 1 below:

[0028] Table 1

[0029] Raw materials Manufacturer Specification / Batch number 2'-Fucosyllactose Hongmo Biotech Z006202403002 Lactobacillus acidophilus NCFM (for over 1 year old) Danisco 200 billion / g Bifidobacterium animalis subsp. lactis Bi-07 Danisco 300 billion / g Lactobacillus rhamnosus GG Shanghai Runxing Biotechnology Co., Ltd. 30 billion / g Bifidobacterium animalis Bb-12 Shanghai Runxing Biotechnology Co., Ltd. 30 billion / g

[0030] Example 1 A partially hydrolyzed formula food composition with anti-allergy function

[0031] It is composed of the following raw materials:

[0032] Table 2

[0033]

[0034]

[0035] Example 2 A partially hydrolyzed formula food composition with anti-allergy function is composed of the following raw materials:

[0036] Table 3

[0037]

[0038]

[0039] Example 3 A partially hydrolyzed formula food composition with anti-allergy function

[0040] It is composed of the following raw materials:

[0041] Table 4

[0042]

[0043] Comparative Example 1 A partially hydrolyzed formula food composition with anti-allergy function

[0044] Different from Example 1, 0.0375 g of Lactobacillus acidophilus NCFM and 0.025 g of Bifidobacterium animalis subsp. lactis Bi-07 were all replaced with 0.0625 g of 2'-fucosyllactose, and the formula is as shown in Table 5 below:

[0045] Table 5

[0046]

[0047]

[0048] Comparative Example 2 A partially hydrolyzed formula food composition with anti-allergy function

[0049] Different from Example 1, 0.0375 g of Lactobacillus acidophilus NCFM and 0.025 g of Bifidobacterium animalis subsp. lactis Bi-07 were replaced with 0.075 g of Lactobacillus acidophilus NCFM (ensuring the same bacterial viability, i.e., 150 U / 100 g). The formula is as shown in Table 6 below:

[0050] Table 6

[0051]

[0052]

[0053] Comparative Example 3 A partially hydrolyzed formula food composition with anti-allergy function

[0054] Different from Example 1, 0.0375 g of Lactobacillus acidophilus NCFM and 0.025 g of Bifidobacterium animalis subsp. lactis Bi-07 were replaced with 0.05 g of Bifidobacterium animalis subsp. lactis Bi-07 (ensuring the same bacterial viability, i.e., 150 U / 100 g). The formula is as shown in Table 7 below:

[0055] Table 7

[0056]

[0057] Comparative Example 4 A partially hydrolyzed formula food composition with anti-allergy function

[0058] Different from Example 1, 0.0375 g of Lactobacillus acidophilus NCFM was replaced with 0.25 g of Lactobacillus rhamnosus GG; 0.025 g of Bifidobacterium animalis subsp. lactis Bi-07 was replaced with 0.25 g of Bifidobacterium bifidum Bb-12 (ensuring the same bacterial viability, i.e., 150 U / 100 g). The formula is as shown in Table 8 below:

[0059] Table 8

[0060]

[0061] Effect experiment

[0062] 1. Fecal treatment

[0063] Sterilize sampling tools such as fecal samplers, disposable gloves, forceps, kraft paper, self-sealing bags, EP tube centrifuge tubes, and reagent bottles in advance. In an anaerobic operation box, equally mix the fresh feces of 3 infants with allergic eczema collected, and take 10 g of the mixed fecal sample and add it to 100 mL of sterile phosphate buffer solution (PBS, pH 6.8) for dilution at a ratio of 1:10. Filter the diluted fecal liquid with 4 layers of sterile gauze, and take the filtrate as the bacterial source liquid. The collection and treatment of all fresh fecal samples need to be completed within 1 h.

[0064] 2. In vitro fermentation

[0065] Method for preparing the basic fermentation broth: Weigh 0.1 g of NaCl, 0.1 g of KCl, 0.01 g of CaCl2, 2.0 g of NaHCO3, 2.0 g of peptone, 2.0 g of yeast extract, 0.5 g of cysteine hydrochloride, 0.5 g of cholate, 0.04 g of K2HPO4, 0.04 g of KH2PO4, 0.02 g of hemin, 0.01 g of MgSO4·7H2O, and 0.001 g of resazurin, add deionized water to 1000 mL, and sterilize at 121 °C for 20 minutes to obtain the fermentation medium.

[0066] Prepare the food compositions prepared in Examples 1-3 and Comparative Examples 1-4 above into a 1% (w / v) solution as the experimental group, and the basic fermentation broth as the control group. Each group is inoculated with the bacterial source liquid at an inoculation amount of 1:9 (v:v), and cultured in an anaerobic incubator at 37 °C for 24 h. Each experiment has 3 replicates.

[0067] 3. Determination of pH

[0068] Samples are taken from the blank control group and the experimental group at 0, 6, 12, and 24 h of anaerobic fermentation, and the pH of the samples is measured using a pH meter.

[0069] 4. Detection of microorganisms

[0070] Before fermentation and after 24 h of fermentation, the detection of viable counts of Lactobacillus is carried out in accordance with GB 4789.35-2023 National Food Safety Standard Food Microbiology Examination - Lactic Acid Bacteria Examination; the detection of viable counts of Bifidobacterium is carried out in accordance with GB 4789.34-2016 National Food Safety Standard Food Microbiology Examination - Bifidobacterium Examination; the detection of viable counts of coliforms is carried out in accordance with GB 4789.3-2016 National Food Safety Standard - Food Microbiology Examination, Enumeration of Coliforms (Second Method); the detection of viable counts of Staphylococcus aureus is carried out in accordance with GB 4789.10-2016 National Food Safety Standard - Food Microbiology Examination, Examination of Staphylococcus aureus (Second Method).

[0071] 5. Statistical analysis

[0072] The data were processed and analyzed using the SPSS 22.0 application software, and the results were expressed as "mean ± standard deviation". One-way analysis of variance (one-way ANOVA) was used, and Duncan's method was used for multiple comparisons. The results were expressed as mean ± standard deviation, and P < 0.05 indicated that the difference was statistically significant.

[0073] Experimental results

[0074] 1. pH change

[0075] Table 9

[0076]

[0077]

[0078] Note: ** indicates extremely significant difference compared with 0 h, p < 0.01.

[0079] As can be seen from Table 9, compared with the control group, the pH of each group decreased significantly after 6 h and 12 h of fermentation. Among them, the decrease amplitude of Example 1 was the largest; after the fermentation ended (24 h of fermentation), the pH of the composition prepared in Example 1 was the lowest, which was 4.06, indicating that the acid-producing ability of the flora of the composition prepared in Example 1 was the strongest.

[0080] 2. Change in viable count of microorganisms

[0081] Table 10

[0082]

[0083] As shown in Table 10, compared with the control group, the viable counts of Lactobacillus and Bifidobacterium in each experimental group increased significantly before and after fermentation, while the viable counts of coliforms and Staphylococcus aureus decreased significantly. Among them, the increases in Lactobacillus and Bifidobacterium in the composition prepared in Example 1 were the most, which were 6.38 CFU / mL and 6.45 CFU / mL respectively; the decreases in the viable counts of coliforms and Staphylococcus aureus were the most, which were 5.93 CFU / mL and 5.82 CFU / mL respectively, indicating that after adding the composition obtained in Example 1, the structure of intestinal microorganisms in the feces of allergic infants can be significantly improved, and its effect is better than other schemes.

[0084] The partially hydrolyzed formula food composition prepared in Example 1 of the present application was detected for peptide segment distribution according to the GB / T 22492 standard. After detection, the content ratio of oligopeptides with a molecular weight of 1000 - 10000 Da in this partially hydrolyzed formula food reached more than 25%.

[0085] The above detailed description is a specific description of one of the feasible embodiments of the present invention, and this embodiment is not intended to limit the patent scope of the present invention. It should be noted that all equivalent implementations or changes made without departing from the present invention should be included within the scope of the technical solution of the present invention. Therefore, the protection scope of the present invention shall be subject to the appended claims.

Claims

1. An anti-allergic special medical infant milk protein partially hydrolyzed formula food composition, characterized in that: The invention comprises the following raw materials per 100 g: 44-55 g lactose, 20-25 g compound vegetable oil, 20-25 g moderately hydrolyzed whey protein, 1-2 g choline chloride, 1-2 g calcium hydrogen phosphate, 0.5-1.5 g 2'-fucosyllactose, 0.6-1 g mono- and di-glycerol fatty acid esters, 0.6-1 g potassium chloride, 0.5-1 g sodium octenylsuccinate starch, 0.4-1 g solid corn syrup, 0.4-1 g tricalcium phosphate, 0.4-1 g maltodextrin, 0.3-1 g arachidonic acid oil, 0.2-0.5 g magnesium sulfate, 0.2-0.5 g docosahexaenoic acid oil, 0.1-0.2 g calcium carbonate, 0.05-0.1 g mixed nucleotides, 0.05-0.1 g Lactobacillus acidophilus NCFM, and 0.05-0.1 g animal Bifidobacterium Bi-07 0.02-0.05g, complex vitamins 0.05-0.1g and complex minerals 0.05-0.1g.

2. The partially hydrolyzed formula food composition according to claim 1, characterized in that The mass ratio of the 2'-fucosyllactose, Lactobacillus acidophilus NCFM and Bifidobacterium animalis Bi-07 is 100-300:3-9:4-8.

3. The partially hydrolyzed formula food composition according to claim 1, characterized in that The mass ratio of the 2'-fucosyllactose, Lactobacillus acidophilus NCFM and Bifidobacterium animalis Bi-07 is 180:7.5:

5.

4. The partially hydrolyzed formula food composition according to claim 1, characterized in that Based on 100g, it includes the following raw materials:

5. The method for preparing the partially hydrolyzed formula food composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: Mix according to the recipe.

6. Use of the partially hydrolyzed formula food composition according to any one of claims 1 to 4 or the partially hydrolyzed formula food composition prepared by the preparation method according to claim 5 in the preparation of anti-allergic products.

7. The use according to claim 6, characterized in that: The products include food, health products or medicines.

8. The use according to claim 7, characterized in that: The food includes candy flakes, soy milk, yogurt, canned food, biscuits, chocolate, cakes, cream, cheese, milk powder, formula milk powder, ice cream, jam, puree, candied fruit, preserved fruit, bread, egg rolls, protein drinks, solid drinks, lactic acid bacteria drinks, plant protein drinks, carbonated drinks, coffee or puffed food.

9. The use according to claim 8, characterized in that: The food also includes nutritionally acceptable nutritional additives.

10. The use according to claim 9, characterized in that: The nutritional additives include, but are not limited to, one or more of dietary fiber, prebiotics, protein, lipids, minerals, and vitamins.

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