Preparation of freeze-dried Lactobacillus acidophilus powder

Abstract

The invention discloses a preparation method of freeze dried microbial powder of Lactobacillus acidophilus, which comprises the technical processes of fermented cultivation of Lactobacillus acidophilus, separation of a fermented fluid, emulsification of microbial soil and vacuum freeze drying of an emulsified solution. The preparation method is characterized in that the strain fermented cultivation comprises the steps as follows: a lactobacillus strain is inoculated into a fluid culture medium in a triangular flask, cultivated at the temperature of 30 to 40 DEG C for 10 to 30 hours, inoculated into a large fermentation flask and fermentation cultivated at the temperature of 35 to 40 DEG C for 10 to 30 hours, and sterilized at the temperature of 115 DEG C for 15 minutes; and fermentation and augmentation cultivation is carried out for 10 to 30 hours, the pH value of the fermented fluid is 5.5 to 6.5, and OD600 is over 2.0; the vacuum freeze drying of the emulsified solution comprises the steps: the emulsified solution is pre-frozen at the temperature of minus 60 to minus 40 DEG C for 1 to 5 hours, and then vacuum frozen and dried at the temperature of minus 60 DEG C and with the vacuum degree of 1 to 8 Pa for 10 to 30 hours. The freeze dried microbial powder of Lactobacillus acidophilus with the total plate count of 1.0 multiplied by 10 cfu / g can be preserved for 18 months at the temperature of minus 18 DEG C with unaffected liveness and stability.

Description

technical field

[0001] The invention discloses a preparation method of Lactobacillus acidophilus freeze-dried bacterial powder, which is applied in the field of biotechnology, is a high-efficiency, low-cost production technology of Lactobacillus acidophilus freeze-dried bacterial powder, and is a development of Lactobacillus acidophilus products It provides necessary technical support for its application in dairy products, food, medicine, etc. Background technique

[0002] Lactobacillus acidophilus (Lactobacillus acidophilus) is Lactobacillus (Lactobacillus), Gram-positive, does not produce spores, does not move, does not have flagella, capsule, sporeless elongated bacteria, does not move, in single, double or Anaerobic or facultative anaerobic microorganisms that exist in the form of short chains and can ferment sugars to produce lactic acid, ethanol, hydrogen peroxide, etc. The G+C content is 36.0%-37.4%, the optimum pH is 5.5-6.0, the optimum growth temperature is 35-38°...