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Method for preparing chitosan oligosaccharide by applying complex enzyme

A composite enzyme and chitosan oligosaccharide technology, applied in biochemical equipment and methods, enzymes, hydrolase and other directions, can solve the problems of incomplete degradation and excessive degradation, and achieve the effects of improved efficiency, high degradation efficiency and high yield

Active Publication Date: 2013-01-09
HAINAN ZHENGYE ZHONGNONG HIGH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, chitosan is degraded by physical methods, chemical methods, etc. to prepare chitosan, or due to incomplete degradation, there are too many high-polymerization chitosan in the product, such as oligomeric chitosan with a polymerization degree greater than 50 Sugar accounts for the majority, or it is degraded too much, so that there are too many glucosamine monomers in the product, or there are too many chitosan oligosaccharides with a degree of polymerization of 2-10. In order to obtain chitosan oligosaccharides with a high degree of polymerization, There are certain defects in the current preparation technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: the preparation of compound enzyme I

[0052] Mix 20 g of cellulase, 10 g of lysozyme, 30 g of amylase, 90 g of lipase, 25 g of glucose, and 15 g of papain to prepare compound enzyme I.

Embodiment 2

[0053] Embodiment 2: the preparation of compound enzyme II

[0054] Mix 25 g of cellulase, 5 g of lysozyme, 20 g of amylase, 90 g of lipase, 30 g of glucose, and 10 g of papain, and add 317 g of vitamin C to prepare compound enzyme II.

Embodiment 3

[0055] Embodiment 3: the preparation of compound enzyme III

[0056] Mix cellulase 15g, lysozyme 15g, amylase 40g, lipase 80g, glucose 20g, papain 20g, add 0.155g of CoSO 4 , 0.151g of MnSO 4 , 0.152g of FeSO 4 , 0.12g of MgSO 4 , 0.161g of ZnSO 4 , 0.174g of K 2 SO 4 , to prepare complex enzyme III.

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PUM

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Abstract

The invention relates to a method for preparing a chitosan oligosaccharide by applying a complex enzyme. The complex enzyme comprises cellulose, lysozyme, amylase, lipase, glucolase and papain. The invention further provides a method for preparing a chitosan oligosaccharide by applying the complex enzyme. The method comprises the following step of: adding the complex enzyme into a reaction buffersolution of chitosan, wherein the mass ratio of the complex enzyme to the chitosan is (1-20):100, and is preferably (5-15):100. The complex enzyme and the chitosan oligosaccharide prepared from the complex enzyme have uniform molecular weight distribution and high yields.

Description

technical field [0001] The invention belongs to the field of preparation methods of oligosaccharides, in particular to a method for preparing chitosan oligosaccharides using complex enzymes. Background technique [0002] Chitooligosaccharide is a very valuable natural polysaccharide, which has broad application prospects in medicine and agriculture, but for the preparation of chitooligosaccharide, especially the preparation of chitooligosaccharide by biological method, the existence process is complicated, the degradation efficiency is low, and the product composition Diversification and other characteristics. [0003] For example, the current scheme of using compound enzymes to prepare chitosan oligosaccharides mainly uses amylase, protease and chitosanase to form compound enzymes in a certain proportion, such as the compound enzyme preparation disclosed in the patent application CN101200711A, which is obtained by subtilis For the enzymes obtained by Bacillus fermentation,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00C12N9/42C12N9/36C12N9/26C12N9/20C12N9/50C12N9/24C12P19/14C12P19/12C12P19/04
Inventor 张善学
Owner HAINAN ZHENGYE ZHONGNONG HIGH TECH
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