Bio-control bacterium nutritional tablet

A nutritional and bio-control bacteria technology, applied in the direction of biocide, bactericide, biocide, etc., can solve the problems of poor colonization ability of post-bio-control bacteria, high cost of structure identification, lack of nutrients, etc., to reduce pesticide application The effect of frequency, low cost and simple process

Inactive Publication Date: 2015-11-04
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The defects in the above three patent applications are: in the first patent, the secondary metabolites of microorganisms are used as pesticides or their lead compounds, and the exact structure of the secondary metabolites needs to be clarified. The main role in the fermentation broth of the 0116 strain is The secondary metabolites of bacterial strains, while the separation, purification, and structural identification of the components of the fermentation broth are costly and take a long time
Due to the lack of nutrients in the dosage form, the colonization ability of the biocontrol bacteria after application is poor and the persistence period is short

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] Step 1: Culture 0116 strain on PDA medium at 28°C for 7 days to prepare spore suspension, and inoculate 10% of the spore suspension into the seed medium. The culture conditions are: 28° C., 160 rpm, and culture for 5 days.

[0032] The seed medium is: 3% peanut cake powder, 4.5% starch, 1.5% glucose, 0.75% sodium chloride, 0.3% alcohol master paste, 0.9% ammonium sulfate, 0.03% potassium dihydrogen phosphate, 0.375% calcium carbonate, 0.105% Ferrous sulfate, 0.105% zinc sulfate, 3% sucrose, 0.3% fructose, pH natural;

[0033] Step 2: Mix the seed culture solution and diatomaceous earth at a ratio of 1:2.

[0034] Step 3: Add 15% sucrose and 15% peanut cake powder and mix well.

[0035] Step 4: Add 6% corn starch or polyethylene glycol 6000 and mix well.

[0036] Step 5: Dry the above mixture in a forced air drying oven until it is nearly dry, the condition is: 28°C, 6~12h.

[0037] Step 6: Mix the dried product evenly, pulverize it, and press it into tablets to obta...

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PUM

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Abstract

The invention discloses a bio-control bacterium nutritional tablet, which comprises the following components in percentage: diatomite (determined as a carrier, and m (diatomite): V (fermentation liquor) = 2:1), 15% of sucrose (as a carbon source), 15% of peanut meal (as a nitrogen source), and 6% of corn starch or polyethylene glycol 6000 (as an adhesive). Moreover, the storage stability of strain 0116 nutritional tablets is determined by periodically measuring the viable count by using a plate count method, and results show that the viable count of the strain 0116 nutritional tablets after being stored for 4 months at a temperature of 4 DEG C is greater than or equal to 10<5>cfu / g. The bio-control bacterium nutritional tablet disclosed by the invention has the characteristics of short development cycle, low cost, simple process and long lasting period, and can be subjected to mass production. Because strain 0116 propagules are taken as the main components of the nutritional tablet, the nutritional tablet can be subjected to mass production, so that compared with the fermentation liquor of strains 0116, the field usage is greatly reduced, thereby reducing the packaging and transportation costs.

Description

technical field [0001] The invention relates to a bio-control bacterium nutritional tablet for preventing and controlling plant diseases. Background technique [0002] At present, the control of plant diseases is mainly based on chemical pesticides, and the long-term use of chemical pesticides has caused great selection pressure on pathogenic bacteria, and pathogenic bacteria are prone to develop drug resistance. At the same time, the high residue and high pollution of chemical pesticides have a serious impact on the environment and the safety of people and animals. Therefore, bio-pesticides with low toxicity and low residue have become a hot spot for development. Hebei Agricultural University applied for three patents on the induced strain 0116 of Streptomyces roseoflavus (Streptomyces roseoflavus) Men-myco-93-63 on November 2, 2005, June 9, 2009 and November 22, 2011 , which are respectively "a biological fungicide and its preparation method, authorized, patent number: 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00A01N63/02A01P3/00
Inventor 李亚宁冯龙赤国彤刘大群魏娇洋张汀
Owner HEBEI AGRICULTURAL UNIV.
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