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Nutritional type biological tablet

A biological tablet and nutritional technology, applied in the direction of biocide, biocide, animal repellent, etc., can solve the problems of decreased antibacterial activity, low bacterial content, high cost, etc., to reduce packaging and transportation costs, and prolong the lifespan. Effective period and short development cycle

Inactive Publication Date: 2015-11-04
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have found that the use of fermented liquid for field experiments shows that the stability of the liquid dosage form is poor, the antibacterial activity decreases during storage, it is not easy to preserve, and the frequency of application is high; although the solid fermentation product can be directly applied and the post-treatment is simple, it will There are still some problems in its large-scale use in the prevention and control of plant diseases, such as too much dosage, low bacterial content, easy pollution, and high cost

Method used

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  • Nutritional type biological tablet
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Embodiment Construction

[0034] Step 1: Transfer the bacteria stored at 4°C to the NA plate medium, incubate at 28°C for 24 hours, prepare the bacterial suspension, and transfer 2mL of the bacterial suspension into a 500mL Erlenmeyer flask containing 100mL of BPY fermentation medium. Cultivate at 30°C and 200r / min for 48h to obtain seed solution.

[0035] Step 2: mix the diatomaceous earth and the seed solution evenly with a mass volume ratio of 1:1.5;

[0036] Step 3: Add 15% glucose and 30% peanut cake powder to the above sample and mix evenly;

[0037] Step 4: Add an appropriate amount of 20% starch slurry to make the sample have a certain viscosity;

[0038] Step 5: Bake in a blast oven at 30°C for 10 hours.

[0039] Step 6: Pulverize the sample and press it into tablets to obtain Bacillus amyloliquefaciens X-278 nutritional biological tablet

[0040] Quality difference meets standard

[0041] Storage stability: 200d bacteria content (cfu / g) ≥ 10 7

[0042] The water content is 0.5%.

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Abstract

The invention discloses a nutritional type biological tablet which comprises the following components by weight percent: carrier kieselguhr and bacillus amyloliquefacien X-278 fermentation liquor at a massic volume ratio of 1:1.5, 15% of glucose serving as a carbon source, 30% of groundnut meal serving as a nitrogen source and 20% of starch slurry serving as an adhesive. An accelerated breakdown test is performed on a sample, the viable content of the sample is detected regularly, and the viable content of the sample is kept at 107cfu / g after 200 days. The nutritional type biological tablet has the characteristics of short development period, low cost and simple process, and can be used for performing large-scale production. Since nutrient substances are added, the tablet is capable of realizing self propagation after being applied to soil, so that the field dosage is greatly reduced in comparison with fermentation liquor and solid bacterial manure, the lasting period is prolonged, and the package cost and the transportation cost are lowered.

Description

technical field [0001] The invention relates to a nutritional biological tablet for preventing and controlling plant diseases. Background technique [0002] Formulation processing is a key factor restricting the development of biopesticides, especially in the process of storage and use, the impact of each component in the formulation on the biocontrol activity of live bacteria is a particularly important factor in the processing of biopesticide formulations. Hebei Agricultural University applied for a patent in 2011 as "a solid microbial fertilizer and its preparation method" with the patent number 201110303507.0. The laboratory and field efficacy tests of Bacillus amyloliquefaciens X-278 fermentation liquid and solid bacterial fertilizer have proved that the bacteria has a good control effect on cotton and has a certain growth-promoting effect on cotton. However, studies have found that the use of fermented liquid for field experiments shows that the stability of the liqui...

Claims

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Application Information

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IPC IPC(8): C05G3/00A01N63/02A01P3/00
Inventor 赤国彤魏娇洋李亚宁刘大群冯龙张汀
Owner HEBEI AGRICULTURAL UNIV.
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