Screening method of nutritive salt for pollutant enhanced bioremediation and application

A screening method and technology for pollutants, applied in the field of environmental engineering, can solve problems such as stimulation and strengthening of indigenous microorganisms, and achieve the effect of enhancing long-term stability

Inactive Publication Date: 2017-11-24
BEIJING GEOENVIRON ENG & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0029] In order to solve the problem of stimulation and enhancement of indigenous microorganisms, the present invent

Method used

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  • Screening method of nutritive salt for pollutant enhanced bioremediation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This embodiment is used in a certain chromium-contaminated site in Shandong, specifically as follows:

[0054] Pollutant is Cr slag, yellow in color, Cr 6+ The concentration is 8353mg / kg-9770mg / kg.

[0055] The experimental procedure is described as follows:

[0056] a. Weigh 100g of the contaminated sample and put it into a beaker;

[0057] b. Test the pH of the sample to be tested = 13.20;

[0058] c. Reduce the concentration of hexavalent chromium to below 100mg / kg through chemical reduction stabilization treatment;

[0059] d. Soak and mix in 1000ml deionized water for 6 hours, fully stir and mix every 2 hours, and take the supernatant after standing for 1 hour, which is the leachate;

[0060] e. Inoculate 1-2ml of leaching solution on thioglycolate fluid medium, nutrient broth, and agar powder respectively, and observe the growth of bacterial colonies;

[0061] f. Add 100ml of leaching solution to three shake flasks, add 7g of compound organic fertilizer and u...

Embodiment 2

[0066] This embodiment is used in a certain chromium-contaminated site in Shandong, specifically as follows:

[0067] Pollutants are Cr polluted soil, Cr 6+ The concentration is 376mg / kg-1612mg / kg.

[0068] a. Weigh 100g of the contaminated sample and put it into a beaker;

[0069] b. Test the pH of the test sample to be 10.10;

[0070] c. Reduce the concentration of hexavalent chromium to below 100mg / kg through chemical reduction stabilization treatment;

[0071] d. Soak and mix in 1000ml deionized water for 8 hours, fully stir and mix every 2 hours, and take the supernatant after standing for 2 hours, which is the leachate;

[0072] e. Inoculate 1-2ml of leaching solution on thioglycolate fluid medium, nutrient broth, and TTC nutrient agar to observe the growth of the colonies;

[0073] f. Add 100ml of leaching solution to three shake flasks, respectively add 7g of compound organic fertilizer and urea mixture (C:N:P weight ratio=10:5:2), 7g of methanol and urea to three ...

Embodiment 3

[0078] This embodiment is used in a certain chromium-contaminated site in Shandong, specifically as follows:

[0079] The pollutant is a mixture of Cr slag and soil, Cr 6+ The concentration is 4622 mg / kg-5384 mg / kg.

[0080] a. Weigh 100g of the contaminated sample and put it into a beaker;

[0081] b. Test the pH of the sample to be tested = 11.90;

[0082] c. Reduce the concentration of hexavalent chromium to below 100mg / kg through chemical reduction stabilization treatment;

[0083] d. Soak and mix in 1000ml deionized water for 7 hours, fully stir and mix every 2 hours, and take the supernatant after standing for 1.5 hours, which is the leachate;

[0084] e. Inoculate 1-2ml of leaching solution on nutrient broth, TTC nutrient agar, agar powder, and thioglycolate fluid medium respectively, and observe the growth of the colony;

[0085] f. Add 100ml of leaching solution to three shake flasks, respectively add 7g of compound organic fertilizer and urea mixture (C:N:P weigh...

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Abstract

The invention discloses a screening method of nutritive salt for pollutant enhanced bioremediation. The screening method comprises the steps of 1) adding a culture medium into liquid samples containing pollutants, then, observing the growing situation of floras, and carrying out baseline screening; 2) adding different varieties of nutritive salt of different proportions into the liquid samples containing the pollutants, carrying out shake-flask culture, and testing the amount of microorganism colonies through plate count and an ATP bioluminescence detector; and 3) detecting concentration of the pollutants in the pollutant-containing liquid samples cultured through adding of nutritive salt, evaluating the pollutant enhanced bioremediation effect of nutritive salt according to a result, and acquiring an optimal nutritive salt formula.

Description

technical field [0001] The invention belongs to the technology of microbial enhanced restoration in the technical field of environmental engineering, and specifically relates to a screening method for nutrient salts used in the enhanced microbial restoration of pollutants and the nutrient salts screened out by the screening method, especially related to the enhanced microbial restoration of hexavalent chromium Nutrient screening method. Background technique [0002] In recent years, with the rapid development of the national economy, the treatment of pollutants is a problem that is widely concerned by the current human society. [0003] The "five poisons" in heavy metals are chromium, cadmium, mercury, arsenic and lead. Among them, hexavalent chromium is the most water-soluble and mobile. Hexavalent chromium is generally produced by chromium salt manufacturers, and generally exists in the chromium slag produced by calcium roasting, and exists in the soil and groundwater ar...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/08
CPCB09C1/08B09C1/10
Inventor 吕正勇申坤朱湖地魏丽苗竹倪鑫鑫张亮亮甄胜利刘泽军
Owner BEIJING GEOENVIRON ENG & TECH
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