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Method for rapidly quantitating Microcystis aeruginosa in water

A microcystis aeruginosa, fast technology, applied in measurement devices, color/spectral property measurement, instruments, etc., can solve the problems of lack of quantitative monitoring methods and low efficiency, and reduce the impact and efficiency of environmental safety and inspection personnel's health. High, sample loss recoverable effect

Inactive Publication Date: 2018-02-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, aiming at the lack and low efficiency of algae quantitative monitoring means in my country, to provide a simple, fast and effective rapid quantitative method for Microcystis aeruginosa in water, to measure its biomass, It is of great significance to the monitoring and early warning of algal blooms

Method used

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  • Method for rapidly quantitating Microcystis aeruginosa in water
  • Method for rapidly quantitating Microcystis aeruginosa in water
  • Method for rapidly quantitating Microcystis aeruginosa in water

Examples

Experimental program
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Embodiment 1

[0030] According to the rapid quantitative method of Microcystis aeruginosa of the present invention, at first establish the calibration curve or regression equation between the algae liquid absorbance and Microcystis aeruginosa cells, the specific steps are as follows:

[0031] 1. Algae solution preparation. Use the algae liquid in the logarithmic phase, dilute 1, 3, 5, 8, 10, 30, 50, 80, 100, 300, 500, 800, 1000, 3000, 5000, 8000 times with ultrapure water respectively.

[0032] 2. Cell count. Accurately count the cells of each concentration by the hemocytometer method, repeat 3 times for each sample, and take the average value to obtain the cell concentration C.

[0033] 3. Measure the absorbance. Add the algae liquid of the blank group and different concentration groups into 10ml quartz cuvettes, and measure the absorbance of different concentrations of algae liquid at 220, 360, 420, 520, 640, 680nm wavelengths by a spectrophotometer, with 3 parallel samples for each gro...

Embodiment 2

[0041] According to the calibration curve or regression equation established at the wavelength of 680nm in Example 1, the rapid quantitative method for Microcystis aeruginosa of the present invention is used to measure the algae cell concentration, to study the growth curve of Microcystis aeruginosa, and to compare it with the microscopic counting method ,Specific steps are as follows:

[0042] 1. Algae solution preparation. In a light incubator, culture with sterilized BG-11 medium. The culture conditions are as follows: the algal species in the logarithmic phase are inserted into the newly prepared BG-11 medium for the test, and 3 parallel samples are set up. A 500ml Erlenmeyer flask was used for the test, filled with 200ml of culture solution, and the initial concentration of inoculated algae cells was 7.08*10 5 cells / ml, illuminance 2500lx, culture temperature 25°C, light-dark ratio 12h:12h, shake regularly every day, and take samples on time.

[0043] 2. Cell count. U...

Embodiment 3

[0051] According to the rapid quantitative method of Microcystis aeruginosa of the present invention, research the filtration effect of different filter media to Microcystis aeruginosa, concrete steps are as follows:

[0052] 1. Algae solution preparation. In the light incubator, cultured with sterilized BG-11 medium, and the concentration of algae cells cultivated continuously for 15 days was used for filtration experiment.

[0053] 2. Filtration experiment using inner diameter The medium pressure filter column is wet packed. After the filter column is filled, the filtration experiment is carried out, the filtration flow rate is 1.5m / h, the ionic strength of 1mM KCl, pH=9.0±0.2, and the experiment is carried out at room temperature. The detailed experiment process can be found in [Jin C, Normani S D, Emelko M B. Surface roughness impacts ongranular media filtration at favorable deposition conditions: Experiments and modeling[J].Environmental science&technology,2015,49(13):...

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Abstract

The present invention discloses a method for rapidly quantitating Microcystis aeruginosa in water. The method comprises: preparing a group of Microcystis aeruginosa liquids with different concentrations, determining the absorbance respectively at wavelengths of 220 nm, 360 nm, 420 nm, 520 nm, 640 nm and 680 nm through a spectrophotometer, and counting the algae cells of each sample by using a blood cell plate counting method to obtain the number of the algae cells per unit volume of the algae liquid; determining the absorbance of a blank sample at wavelengths of 220 nm, 360 nm, 420 nm, 520 nm,640 nm and 680 nm, and drawing a calibration curve according to the absorbance and the cell concentration, or obtaining regression equations corresponding to the wavelengths through linear regression; and diluting a Microcystis aeruginosa-containing sample to be measured, determining the absorbance of the algae liquid at the specific wavelengths by using the spectrophotometer, and calculating thecell concentration of the Microcystis aeruginosa in the sample to be measured according to the established calibration curve or regression equation. According to the present invention, with the method, the biomass is determined, such that the important significance is provided for the monitoring and early warning of algal bloom.

Description

technical field [0001] The invention relates to the fields of energy recycling and environmental protection, more specifically, to a method for quantitative monitoring of algae in water, in particular to a rapid quantitative method for Microcystis aeruginosa in water. Background technique [0002] Microcystis aeruginosa is the main algae species of freshwater lake blooms in my country, and it is the dominant algae species during the outbreak of cyanobacteria, and the microcystin synthesized by it is also the most frequent occurrence, the largest amount of production and the most harmful among the known cyanobacteria blooms. The worst algal toxins. At present, drinking water sources in many cities in my country are rivers or lakes, and these water bodies are polluted by algae to varying degrees. Therefore, real-time monitoring of Microcystis aeruginosa is particularly important. [0003] Usually, the quantitative determination methods of Microcystis aeruginosa in water includ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/10G01N21/31
CPCG01N15/10G01N21/314G01N2021/3148G01N2015/1024
Inventor 赵伟高赵鹏田一梅金超崔诺郭蓉
Owner TIANJIN UNIV
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