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Culture medium composition, culture medium and viable bacteria counting method for strain

A technology of culture medium and composition, applied in microorganism-based methods, biochemical equipment and methods, determination/inspection of microorganisms, etc., can solve the problem of inability to distinguish colony morphology

Active Publication Date: 2014-07-09
COFCO NUTRITION & HEALTH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, the detection of yeast usually uses YEPD medium, potato medium, wort medium, Chapei medium, etc. The yeasts growing on these mediums are often very similar in shape, especially in the detection of Saccharomyces cerevisiae It is difficult to accurately count the different strains of Saccharomyces cerevisiae
WL medium is a medium for counting yeast and bacteria. This medium can be used to detect and distinguish some yeast strains of different genera, but for the same genus of Saccharomyces cerevisiae, even if it is from different sources, it cannot Distinguished by differences in colony morphology on one culture plate

Method used

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  • Culture medium composition, culture medium and viable bacteria counting method for strain
  • Culture medium composition, culture medium and viable bacteria counting method for strain
  • Culture medium composition, culture medium and viable bacteria counting method for strain

Examples

Experimental program
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Effect test

Embodiment 1

[0050] This example illustrates the pre-cultivation of different strains of Saccharomyces cerevisiae

[0051] (1) Preparation of YEPD medium

[0052] Accurately weigh 10g of yeast extract, 20g of peptone, and 20g of glucose, and use double-distilled water to make up to 1000mL. After dissolving, use 0.1mol / L sodium bicarbonate to adjust its pH to 6.0, and sterilize at 115°C with moist heat 20min.

[0053] (2) Separate cultivation of different strains of Saccharomyces cerevisiae

[0054] Inoculate CGMCC3284 strain, CGMCC3732 strain, CICC1450 strain, T73 strain, BH8 strain, D254 strain and F15 strain into 100mL of the above-mentioned YEPD medium, and the inoculum size is 10 6 CFU / mL, cultured at 37°C for 18 hours.

[0055] Take 1 mL of each of the above-mentioned Saccharomyces cerevisiae cultured separately to the logarithmic phase, and dilute it with 8.5% by weight saline for 10 6 The corresponding diluted bacterial suspension C1 (CGMCC3284), bacterial suspension C2 (CGMCC37...

Embodiment 2-6

[0067] The following examples are used to illustrate the medium composition and medium provided by the invention

Embodiment 2

[0069]Accurately weigh 15g of glucose, 30mg of diammonium hydrogen phosphate, 0.5g of yeast extract, 4.5g of ammonium bismuth citrate, 5g of proline, 2.5g of sodium sulfite, 5μg of biotin, 500μg of calcium pantothenate, 5μg of folic acid, 5μg of inositol, Niacin 500μg, vitamin B6 250μg, riboflavin 250μg, thiamine hydrochloride 250μg, ergosterol 10mg, magnesium sulfate heptahydrate 10mg, calcium chloride 10mg, zinc sulfate 0.5mg, copper sulfate pentahydrate 100μg and agar powder 18g, add into a 2000mL Erlenmeyer flask, and mix evenly to obtain the culture medium composition A1 of the present invention. Wherein, in terms of nitrogen element, the total content of the nitrogen source is 1.35% by weight, and the nitrogen source contains proline with a total content of 99% by weight in terms of nitrogen element.

[0070] Add double-distilled water to the above-mentioned Erlenmeyer flask containing the medium composition A1 and set the volume to 1000mL, heat to dissolve and adjust th...

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Abstract

The invention discloses a culture medium composition, which contains a nitrogen source. The culture medium composition is characterized in that the nitrogen source contains urea and / or amino acid, in terms of nitrogen element, urea and / or amino acid account for more than 80wt% of the total content of the nitrogen source, and based on the total weight of the culture medium composition, in terms of nitrogen element, the total content of the nitrogen source is 0.02-15wt%. The invention also discloses a culture medium, which contains the culture medium composition provided by the invention and water, wherein the culture medium composition and the water are in a weight ratio of 1:20-30. In addition, the invention discloses a viable bacteria counting method for a strain. The method includes: subjecting the strain to plate culture on the culture medium provided by the invention, and conducting plate counting on the cultured strain. By means of the method provided by the invention, viable bacteria counting on different strains can be carried out according to different colony morphologies and colors.

Description

technical field [0001] The invention relates to a culture medium composition, a culture medium containing the culture medium composition, and a method for counting viable bacteria using the culture medium. Background technique [0002] Winemaking is a complex microbiological process in which yeast is the dominant microorganism. Alcoholic fermentation of wine is mainly the process of converting the nutrients in grape juice into alcohol and other flavor substances under the action of Saccharomyces cerevisiae through natural fermentation, pure fermentation or mixed fermentation. [0003] In the winemaking process, a single strain of Saccharomyces cerevisiae is often used for alcoholic fermentation, but research in recent years has fully shown that wines made by different Saccharomyces cerevisiae strains have great differences in style, quality, color, aroma and taste. There are also more and more studies using different Saccharomyces cerevisiae strains to carry out double-stra...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12Q1/06C12R1/865
Inventor 梁恒宇林海龙
Owner COFCO NUTRITION & HEALTH RES INST
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