RVA-APC combined method for discrimination of ionizing irradiation wheat and flour

A technology of ion irradiation and wheat, applied in the direction of flow characteristics, measuring devices, instruments, etc., can solve the problems of multiple testing procedures, complicated sample processing, and high testing costs, and achieve the effect of reducing costs, simple measurement process, and low cost

Inactive Publication Date: 2015-04-22
NANJING UNIV OF FINANCE & ECONOMICS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, high requirements for instruments and equipment, complex sample processing, many testing procedures, high testing costs, and long testing cycles, etc.

Method used

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  • RVA-APC combined method for discrimination of ionizing irradiation wheat and flour

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for judging ion-irradiated hard winter white wheat comprises five steps. Step 1, take the test wheat sample and unirradiated wheat of the same variety, place of origin and harvest period as a control; Step 2, pretreatment of the sample, grind the sample into flour, and pass through an 80-mesh sieve; Step 3, measure the RVA of the sample, weighing Sample 3.0g, add distilled water 25.0g, then put into RVA measuring instrument and carry out heating measurement, obtain RVA characteristic spectrum in RVA measuring instrument, obtain characteristic spectrum value according to RVA characteristic spectrum; Step 4, sample APC determination; Step 5 Judgment of the results, peak viscosity (1824.7), hot paste viscosity (926.2), final viscosity (1858.5), disintegration value (897.5), reduction value (-34.3) and gelatinization temperature (67.7°C) of the test group, APC value 1.3 ×10 5 cfu / g. The peak viscosity (2393.7), hot pulp viscosity (1299.6), final viscosity (2403.5...

Embodiment 2

[0021] Another method for judging suspected ion-irradiated waxy wheat flour is divided into five steps. Step 1, take the test wheat flour sample and the wheat flour of the same variety as a comparison; Step 2, pre-treat the sample, grind the sample, and pass through an 80-mesh sieve; Step 3, measure the RVA of the sample, weigh 3.0 g of the sample, add 25.0 g of distilled water, and put Enter the RVA measuring instrument and carry out heating measurement, obtain the RVA characteristic spectrum in the RVA measuring instrument, obtain the characteristic spectrum value according to the RVA characteristic spectrum; Step 4, sample APC is measured; Step 5 result judgment, test group peak viscosity (1900.7), Hot paste viscosity (962.7), final viscosity (1876.5), disintegration value (938.1), reduction value (-24.2) and gelatinization temperature (68.1°C), APC value 5.3×10 6 cfu / g, peak viscosity (2135.7), hot pulp viscosity (1109.6), final viscosity (2148.5), disintegration value (10...

Embodiment 3

[0023] The difference with Example 2 is that the control group is flour made from wheat ground of the same variety, place of origin and harvest period, and the APC value of the experimental group is 8.3×10 4 cfu / g, considering that the five RVA characteristic indexes of peak viscosity, hot paste viscosity, final viscosity, disintegration value and reduction value all decreased to a certain extent, while the gelatinization temperature increased, and APC was lower than 10 6 cfu / g, it is determined that the flour sample has been irradiated with ions.

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Abstract

The invention discloses a RVA-APC combined method for discrimination of ionizing irradiation wheat and flour. The method includes: taking test wheat raw grain or flour sample and a wheat raw grain or flour product (unirradiated) of the same variety and producing area to do contrast; conducting sample pretreatment, grinding the sample into flour and performing sieving by a 80-mesh sieve; conducting RVA test on the sample, weighing 2.0-3.5g of the sample, adding 20.0-30.0g of distilled water, and then putting the mixture into a RVA tester to carry out heating determination to obtain 6 main characteristic spectrum values in the RVA tester; performing APC determination on the sample; conducting comparison with the contrast group, if five RVA characteristic indexes, i.e. peak viscosity, through viscosity, final viscosity, breakdown and setback decrease, the pasting temperature rises and the aerobic plate count is less than 10<6> cfu/g, determining that ionizing irradiation occurs yet and the greater the difference is, the higher the received irradiation dose is. The method provided by the invention has the advantages of small detection sample amount, simple operation, fast determination process, and low cost.

Description

technical field [0001] The invention belongs to the technical field of food safety and detection, and specifically relates to a method for discriminating ion-irradiated wheat and flour by combining RVA with APC. Background technique [0002] Ionizing irradiation food technology is a new technology for food processing by using the physical, chemical and biological effects produced by the interaction of ionizing radiation of radioactive substances (mainly cobalt 60) and target substances. Food irradiation technology helps reduce food-borne diseases, reduce post-harvest loss of agricultural products, and extend food shelf life, etc., and has been more and more widely used. The standard research on irradiated food processing and testing in my country is relatively lagging behind. There are roughly 6 irradiated food testing standards that have been promulgated, namely NY / T 1207-2006 Irradiated spices and dehydrated vegetable thermoluminescence identification method; NY / T 1573-200...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N11/14
Inventor 陈银基曹俊蒋伟鑫陈霞沈飞杨玉玲韩启飞
Owner NANJING UNIV OF FINANCE & ECONOMICS
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