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Standard sample for total plate count in milk powder and preparation method thereof

A technology for standard samples and the total number of colonies, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of poor sample uniformity and stability, easy change of bacterial flora, short storage period of samples, etc.

Active Publication Date: 2017-08-25
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If in the total number of colony samples prepared, the flora is prone to change (reproduction and death, etc.), it will lead to short shelf life of the sample, poor uniformity and stability of the sample

Method used

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  • Standard sample for total plate count in milk powder and preparation method thereof
  • Standard sample for total plate count in milk powder and preparation method thereof
  • Standard sample for total plate count in milk powder and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The standard sample of the total number of colonies in milk powder, the target bacteria are Bacillus cereus (Bacillus cereus), Escherichia coli (E.coil), Klebsiella pneumoniae (Klebsiella pneumoniae), Enterobacter cloacae (Enterobactercloacae), gold Staphylococcus aureus (Staphylococcus aureus) composition.

[0028] The sample is based on trehalose, skimmed milk powder and sterile water, wherein the volume fraction of trehalose is 12%, and the volume fraction of skimmed milk powder is 15%.

[0029] The target concentration of the target flora in the sample is 10 4 ~10 5 CFU / mL.

Embodiment 2

[0031] Such as figure 1 Shown, a kind of preparation method of the standard sample of the total number of colonies in the milk powder in a kind of embodiment 1, comprises the selection of sample adding bacterial strain, the preparation of lyoprotectant, sample freeze-drying, sample homogeneity and stability test four steps, Specific steps are as follows:

[0032] 1. Selection of sample addition strains

[0033] Select standard strains according to Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Staphylococcus aureus , all standard strains are purchased from institutions designated by the government, and a strain certificate is attached to ensure the traceability of the strains.

[0034] 2. Preparation of lyoprotectant

[0035] The sample was prepared with trehalose, skimmed milk powder and sterile water as the matrix (volume fraction: trehalose 12%, skimmed milk powder 15%) to prepare a lyoprotectant.

[0036] 3. Sample freeze-drying

[003...

Embodiment 3

[0072] Each step of the preparation method of the standard sample of the total number of bacterial colonies in milk powder described in this embodiment is all the same as in Example 2, and the different technical parameters are: in the preparation of the bacterial suspension, the concentration of each target bacteria in the target flora suspension Concentration according to 5×10 3 CFU / mL preparation; freeze-drying process 52h.

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Abstract

The invention belongs to the field of quality control in the aspect of microbiological test, and particularly relates to a standard sample for total plate count in milk powder and a preparation method thereof. A target flora of the standard sample for total plate count in milk powder is composed of bacillus cereus, escherichia coli, klebsiella pneumonia, enterobacter cloacae and staphylococcus aureus. The uniformity and stability of the standard sample for total plate count in milk powder meet the requirements on the standard sample, the stability of the test sample can be ensured to the max, and even if the content of escherichia coli is changed during transportation and storage, the total plate count is not affected in a statistical sense; and the preparation method for the sample has the advantages of simple process and high success rate.

Description

technical field [0001] The invention belongs to the quality control field of microbiological detection, in particular to a standard sample of the total number of bacterial colonies in milk powder and a preparation method thereof. Background technique [0002] The field of microbiological testing of milk powder is very special. At present, there is no precedent for the preparation of milk powder microbiological standard samples and large-scale production by institutions at home and abroad, which is still a blank field. At present, although some institutions prepare standard samples of microorganisms, the uniformity and stability of the standard samples can hardly meet the requirements, and transportation is extremely difficult; or the standard samples are pure strains containing only target bacteria, which are far from the actual samples Meet the needs of daily laboratory quality control. [0003] The total number of colonies refers to the total number of microbial colonies...

Claims

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Application Information

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IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 卢行安陶德华李宏赵红阳王秀君刘汉霞王伟陈若晞
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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