Detection method of microbiological indexes of xanthan gum and similar gum

A detection method and technology of microorganisms, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve problems such as inability to proceed and insoluble

Inactive Publication Date: 2015-02-18
ORDOS ZHONGXUAN BIOCHEM
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Problems solved by technology

However, if the 25g sample is added to the 225ml buffer solution according to the method specified i...

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Embodiment Construction

[0022] The following are preferred embodiments of the present invention, which do not limit the protection scope of the present invention.

[0023] A detection method for xanthan gum and similar colloidal microbial indicators, characterized in that the detection method is as follows;

[0024] (1) Make plate count agar (plate count agar, PCA) culture medium: add the culture medium raw material that the composition is tryptone 5.0g, yeast extract 2.5g, glucose 1.0g, agar 15.0g to 1000ml distilled water, boil Dissolve, adjust the pH to 7.0±0.2, then dispense into Erlenmeyer flasks, autoclave at 121°C for 25 minutes, then cool to 46°C-48°C and place in a constant temperature drying oven to keep warm;

[0025] (2) Make phosphate buffer: weigh 34.0g potassium dihydrogen phosphate (KH 2 PO 4 ) in 500mL of distilled water, adjust the pH to 7.2 with 1mol / L sodium hydroxide solution, dilute to 1000mL with distilled water and store in the refrigerator as the storage solution; take 1.25...

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Abstract

The invention discloses a detection method of microbiological indexes of xanthan gum and similar gum. The detection method comprises the following steps: making a plate count agar (PCA) culture medium, making a phosphate buffer solution, adding a sample to be tested into the phosphate buffer solution for diluting to prepare a sample plate, culturing the plate in a constant-temperature culture box, counting bacterial colonies on the plate, calculating the total number of bacterial colonies, expressing the total number of bacterial colonies, and forming a report. The detection method overcomes the shortcoming that xanthan gum in GB/T4789 cannot be dissolved, is simple and feasible, is relatively suitable for actual operations of labs in enterprises, and has relatively high values and progressive meanings on the aspect of xanthan gum detection.

Description

technical field [0001] The invention relates to a detection method for indicators of xanthan gum and similar colloidal microorganisms, and belongs to the field of detection methods for colloidal microorganisms. Background technique [0002] At present, under the background of more and more attention to food safety, the content of food additives and microorganisms in additives is a very important detection object in the food industry. As a non-toxic food additive xanthan gum and similar colloids , which plays a very important role in food such as emulsifier, stabilizer and thickener. However, the microbiological indicators of xanthan gum and similar colloids should meet the national standard of food additive xanthan gum-GB13886-2007 when they leave the factory. The detection method of the microbial index of xanthan gum in the national standard is tested according to the method specified in GB / T 4789. This method is not applicable to the thickener such as xanthan gum, which h...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
Inventor 托建刚董金儒
Owner ORDOS ZHONGXUAN BIOCHEM
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