Method for evaluating bacterium killing effect of daily chemical products by ATP bioluminescence lgCA-lgIA standard curve method

Abstract

The invention relates to a method for evaluating bacterium killing effect of daily chemical products. The method comprises detection steps as follows: sample extraction and pretreatment requirement; model selection of equipment and preparation of a reagent and a culture medium; collection and activation of strains and preparation of a bacterial suspension; construction of an ATP concentration log value lgCA-relative fluorescence intensity log value igIA standard curve and viable bacteria ATP concentration CA calibration of an inoculation bacterial solution; neutralizer identification and sterilization test; IA determination of a recovery solution and calculation of viable bacteria ATP concentration CA and TA of the recovery solution; calculation of an antibacterial rate R or antibacterial activity value A; and result evaluation. The method is characterized by applying an ATP fluorophotometer to precisely and quantitatively test the bacterium killing effect of daily chemical products represented by R or A. The method neutralizes and recovers control samples and antibacterial samples after specific time of contamination / killing, determines IA of the recovery solution and represents and calculates R or A by lgIA and provides result evaluation basis. The researched and developed ATP bioluminescence lgCA-lgIA standard curve method for detecting the bacterium killing effect of daily chemical products will support product quality improvement by the scientific and advanced detection technology.

Description

technical field [0001] The invention relates to a method for testing the bacteria-killing effect of daily chemicals, in particular to an ATP bioluminescent method for accurately and quantitatively evaluating the bacteria-killing effect of daily chemicals represented by the sterilization rate R or the sterilization index A by using an ATP fluorescence photometer lgC A -lgI A The standard curve method belongs to the technical field of bactericidal effect evaluation of daily chemicals. Background technique [0002] In recent years, with the improvement of people's living standards and changes in consumption concepts, the domestic market's demand for daily chemical products such as washing and care has increased year by year; according to the "2018-2023 China's FMCG Industry Development Prospects and Investment Opportunity Analysis Report" shows that China's daily chemical consumption market as a whole is showing a continuous growth trend higher than the GDP growth rate. As o...

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Problems solved by technology

Although my country has promulgated and implemented GB15981-1995 "Evaluation Methods and Standards for Disinfection and Sterilization Effects", GB 15979-2002 "Hygienic Standards for Disposable Sanitary Products" and QB / T 2738-2012 "Evaluation of Antibacterial and Antibacterial Effects of Daily Chemical Products Evaluation Methods", QB / T 2739-2005 "Preparation of Test Solutions for Titration Analysis (Volume Analysis) of Common Test Methods for Detergents" and other related standards, but the existing detection methods have the following common problems in technical content and practical application: 1. The reason is that the number of experimental strains involved is too small to meet the company’s new product development and quality control needs to keep pace with the times; the second is that the experimental process is cumbersome, and the relevant operations are affected by the professional experience of the experimenter, and the test error is large and lacks comparability; the third is the test The cycle is between 48 hours and 72 hours, and the time and economic costs are high

At present, the research on antibacterial and disinfection effect detection technology in foreign countries is developing toward the trend of quantification, rapidity and simplification, focusing on the accuracy and comparability of detection results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles - Determination of antibacterial properties of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. The change of ATP content after inoculation is a fluorometric method to characterize the anti-bacteria / mold performance, but the absorption method, transfer method and transfer method provided by it are only suitable for textile materials or microbes with water absorption and control sample / mold growth value > 0 Porous materials; the evaluation of the bacterial killing effect of daily chemical products must form a set of clear and specific methods for key technical aspects such as neutralization effect evaluation and result calculation formula, and at the same time, it is necessary to provide relevant measurement uncertainty evaluation

In addition, the characterization parameters of the standard method’s bactericidal effect are relatively single, only involving the antibacterial activity value A, while the usual killing rate R in my country is

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