Method for detecting anti-fungal performance of nano inorganic material by ATP bioluminescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting anti-fungal performance of a nano inorganic antibacterial material. The method for detecting the anti-fungal performance of the nano inorganic antibacterial material comprises the steps that sample preparation and pretreatment are carried out; equipment model selection and reagent and culture medium preparation are carried out; culture preservation, excitation and bacterium suspension preparation are carried out; viable bacterium content C blood cell plate counting of a bacterium suspension after methylene blue staining is carried out; an ATP concentration log value lgC-relative fluorescence intensity log value lgI standard curve is established and an inoculating bacterium solution C is calibrated; sample inoculation and cultivation are carried out; recycled solution I measurement and ATP concentration C and T calculation are carried out; an antibacterial ratio R or an antibacterial activity value A is calculated; and a result is evaluated. The method for detecting the anti-fungal performance of the nano inorganic antibacterial material is characterized in that an ATP fluorophotometer is used for accurately and quantitatively testing the anti-fungal performance of the nano inorganic material represented by R or A. According to the method for detecting the anti-fungal performance of the nano inorganic antibacterial material, it is stipulated that control samples and antibacterial samples after shake cultivation are recycled, and I of the control samples and the antibacterial samples is measured and lgI is used for representing and calculating R or A; and a result evaluation basis is provided. According to an ATP bioluminescence lgC-lgI standard curve method for detecting the anti-fungal performance of the nano inorganic material, an advanced detection technology can be used for supporting product quality improvement.

Description

Technical field [0001] The invention relates to a method for testing the antifungal performance of nano inorganic antibacterial materials, in particular to an ATP biofluorescence that uses an ATP fluorescence photometer to accurately and quantitatively detect the antifungal performance of nano inorganic antibacterial materials characterized by antibacterial rate R or antibacterial activity value A lgC A -lgI A The standard curve method belongs to the technical field of antibacterial function detection of nano-inorganic materials. Background technique [0002] In recent years, with the rapid progress of social science and technology and the increasing awareness of people’s health and environmental protection; nano-inorganic antibacterial materials are widely used in many fields such as building materials, home appliances, textiles, food bags, daily chemicals, and their domestic market scale has already exceeded 100 billion. And it is increasing year by year, but the current lag of...

AI Technical Summary

Problems solved by technology

In view of the current development trend of quantification, rapidity and simplification in the research field of foreign antibacterial material performance testing technology, the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (textiles-antibacterial finishing textiles) Determination of antibacterial performance of textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products.Part1 Luminescence (textiles - Determination of antifungal performance of antibacterial finishing textiles)", which stipulates that the change of ATP content after sample inoculation is used to characterize Fluorescence measurement method for anti-bacteria / mold performance, but it is only applicable to textile materials or microporous materials with water absorption and control sample / mold growth value > 0, and it is not suitable for key technologies such as the concentration of inoculum solution and the effective conditions of the test. Applicable to nano-scale powder inorganic antibacterial materials with non-water absorption and fungal growth value of the control sample <0; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

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