Method for rapidly detecting mycobacterium

A mycobacterium and detection method technology, applied in the field of microbial detection, can solve the problems of low sensitivity, aggravate drug-resistant strains, and delay the rational treatment of patients, and achieve high sensitivity and environmental protection effects

Inactive Publication Date: 2010-02-03
SHANGHAI MEIQUAN SCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the slow growth of Mycobacterium tuberculosis, the traditional Roche medium culture usually takes 4-6 weeks, which is not only time-consuming, but also has low sensitivity, and delays the rational treatment of patients, and irregular drug use aggravates the emergence of drug-resistant strains

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0010] The following examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. For the experimental methods that do not specify specific conditions in the examples, usually follow the conventional conditions or the conditions suggested by the manufacturer.

[0011] The invention adopts the ATP bioluminescent detection technology, compares the initial ATP value with the ATP value measured after culturing for a period of time, and adds acid-fast staining to preliminarily judge the growth of mycobacteria.

[0012] 1. Sample Collection, Processing and Inoculation

[0013] Patient specimens were inoculated into Middlebrook 7H9 liquid medium (produced by BD Company, USA) after digestion and decontamination treatment by conventional NALC-NaOH method if contamination was suspected.

[0014] Patient samples here can be selected from: sputum, bronchoalveolar lavage fluid, bronchial flushing fluid, gastric flushing fluid...

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Abstract

The invention provides a method for rapidly detecting mycobacterium. In the method, ATP bioluminescence technology is used for detection, and the growth condition of the mycobacterium is judged by comparing the initial ATP value with the ATP value measured after a cultivation period and by adding acid-fast stain. Compared with the prior art, the method uses the rapid and sensitive ATP bioluminescence technology for detecting the growth condition of the mycobacterium in a liquid culture medium, and simultaneously combines the acid-fast stain to primarily judge whether the growth of the mycobacterium exists to achieve the characteristics of high speed, high sensitivity, and the like. Generally, the report time of the positive detection result is no more than 2 to 3 weeks.

Description

technical field [0001] The invention relates to the field of microorganism detection, in particular to a rapid detection method for mycobacteria. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (referred to as Mycobacterium tuberculosis) mainly through the respiratory tract. About 2 billion people in the world are currently infected with Mycobacterium tuberculosis. China is one of the 22 countries with a high incidence of tuberculosis, and the number of tuberculosis patients ranks second in the world. Currently there are 4.5 million active tuberculosis patients, including 1.5 million infectious tuberculosis patients, and about 1.45 million new tuberculosis patients occur each year. 80% of the patients are in rural areas. Mycobacterium tuberculosis remains the "gold standard" for TB diagnosis and the basis for drug susceptibility testing. The traditional tuberculosis drug susceptibility test is carried out on ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/76C12Q1/02
Inventor 彭志刚
Owner SHANGHAI MEIQUAN SCI & BIOTECH
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