Integrated micro-fluidic chip for detecting bacteria total amount and bacterial spore amount

Abstract

The invention discloses an integrated micro-fluidic chip for detecting the bacteria total amount and the bacterial spore amount and application thereof. The integrated micro-fluidic chip for detectingthe bacteria total amount and the bacterial spore amount comprises two independent channels which are separately used for an ATP bioluminescence method and DPA fluorescence detection, a sample is divided into two parts after entering the channels, one part passes through a splitting decomposition area, a washing area and a reaction area, the other part passes through a splitting decomposition area, a washing area, a reaction area and a fluorescence excitation area, and finally the bacteria total amount and the bacterial spore amount are measured and determined by an optical detection system.The integrated micro-fluidic chip for detecting the bacteria total amount and the bacterial spore amount can be operated in a one-button mode, has the advantages of integration, high sensitivity and the like, and can rapidly, conveniently and simultaneously detect the bacteria total amount and the bacterial spore amount.

Description

technical field [0001] The invention relates to the technical field of designing microfluidic chips, and in particular relates to an integrated microfluidic chip that can be used in spacecraft to simultaneously detect the total amount of bacteria and bacterial spores. Background technique [0002] Bacterial pollutants on spacecraft flying from the earth to other planetary bodies may affect the on-site life detection experiment of the target celestial body and the returned celestial body planet samples. The total amount of bacteria and the amount of bacterial spores that endure extreme environments such as strong radiation and dryness, and then use appropriate methods to disinfect them to maintain a high-clean environment of the spacecraft. The current spacecraft bacteria detection is the use of microbial culture technology. The use of this method for microbial detection of spacecraft has certain shortcomings: First, the accuracy is insufficient, because some microorganisms a...

AI Technical Summary

Problems solved by technology

The use of this method for microbial detection of spacecraft has certain shortcomings: First, the accuracy is insufficient, because some microorganisms are not cultivable, and it is easy to underestimate the total number of microorganisms in the spacecraft.

[0006] In the current existing research, there have been reports of microfluidic chips based on ATP bioluminescence for bacterial detection, but so far, there has been no combination of ATP bioluminescence and DPA fluorescence, which can simultaneously detect the total amount of bacteria and Integrated microfluidic chip report of bacterial spores

Images