Method for detecting antibacterial performance of antibacterial plastics by ATP bioluminescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting the antibacterial performance of antibacterial plastics. The method comprises the following detection steps of: carrying out sample preparation and preprocessing; carrying out equipment model selection and reagent and culture medium preparation; carrying out culture preservation and activation, and preparing bacterial suspension; establishing an optical density (OD) value-viable bacterium content logarithm value lgC standard curve, and carrying out inoculation bacteria solution C calibration; establishing a lgC-relative fluorescence intensity logarithm value lgC standard curve; carrying out sample inoculation, culture, elution and recovery; measuring recovery liquid I, and reckoning the viable bacterium contents C and T of the recovery liquid; calculating an antibacterial ratio R or an antibacterial activity value A; and carrying out result evaluation. The method is characterized in that an adenosine triphosphate (ATP) fluorophotometer is applied to accurately and quantitatively test the plastic antibacterial performance represented by R or A. The method stipulates that control samples and antibacterial samples subjected to 0h contact and cultured for 24h are eluted and recovered, the recovery liquid I is measured, R or A is represented and calculated by lgI, and a result evaluation basis is provided. The ATP bioluminescence lgC-lgI standard curve method researched and developed by the invention for detecting the antibacterial performance of the plastics supports product quality improvement by a scientific and advanced detection technology.

Description

technical field [0001] The invention relates to an antibacterial performance testing method of antibacterial plastics, in particular to an ATP bioluminescent IgC that uses an ATP fluorescence photometer to accurately and quantitatively detect the antibacterial performance of antibacterial plastics characterized by antibacterial rate R or antibacterial activity value A B -lgI B The standard curve method belongs to the technical field of plastic antibacterial function detection. Background technique [0002] Plastic products are widely used in the fields of industry and people's livelihood. Because the additives used in the processing process provide the necessary nutrient source for the growth of bacteria, molds and other microorganisms, the sterility of plastics and their surfaces is very important for reducing the spread of diseases and improving people's health. level is very important. Since the 1990s, developed countries represented by Japan and the United States have ...

AI Technical Summary

Problems solved by technology

However, the existing detection methods have the following common problems in technical content and practical application: first, the number of experimental bacteria involved is too small to meet the needs of enterprises for new product development and quality control that keep pace with the times; second, the experimental process is cumbersome and related operations Affected by the professional experience of the experimenter, the test error is large and lacks comparability; the third is that the test cycle is between 48 hours and 72 hours, and the time and economic cost are high

At present, the research on performance testing technology of antibacterial materials in foreign countries is developing towards quantification, rapidity and simplification, focusing on the accuracy and comparability of test results; the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of Textile products (textiles - Determination of antibacterial performance of antibacterial finishing textiles)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products. Fluorescence assay is used to characterize the anti-bacteria / mold performance by the change of ATP content of the sample after inoculation, but it is only applicable to textile materials or microporous materials with water absorption and the growth value of bacteria / mold in the control sample>0. The key technical aspects such as the effective conditions of the test are not applicable to materials such as plastics and glass with non-absorbent hard surfaces and the bacterial growth value of the control sample <0; at the same time, no clear result calculation formula and measurement uncertainty evaluation are provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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