Method for quick detection of microbe

A microbial and rapid technology, applied in the field of microbial detection, can solve problems such as several days or even more than a week, and achieve the effect of strong specificity and high sensitivity

Inactive Publication Date: 2006-09-06
益思美诠生物科技(上海)有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the traditional culture method has high sensitivity and specificity, it usually takes several days or even more than a week to report the detection results

Method used

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Examples

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Embodiment Construction

[0009] Specific embodiments of the present invention: rapid detection of Escherichia coli O157:H7 in food by immunomagnetic bead separation plus ATP bioluminescence technology:

[0010] 1. Sample collection, enrichment and immune magnetic bead enrichment: the collected food samples, such as 25 grams of beef, etc. were treated according to the conventional national standard method, and then enriched and cultured, and then coated with anti-Escherichia coli O157:H7 monoclonal or Add 15 μl of immunomagnetic beads of polyclonal antibodies into a test tube containing 0.9 ml of enrichment culture, mix them with a sample mixer and place them in a magnetic concentrator to remove the suspension; Magnetic beads are resuspended in buffer for ATP detection.

[0011] 2. ATP detection: add the lysing agent containing trichloroacetic acid (TCA) into the resuspended suspension of immunomagnetic beads combined with E. coli O157:H7 according to an equal volume, and add luciferin-luciferase after...

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Abstract

The provided method comprises: with specific immune magnetic pearl to capture the special microbe in sample; releasing the ATP in microbe cell with cracking agent; finally, detecting the ATP to decide the target and opposite amount with the fluorescein-luciferase bioluminescence system. Compared with prior art, this invention has high sensitivity and strong specificity to complete whole process within 8h.

Description

Technical field: [0001] The invention relates to a method for rapid detection of microorganisms, in particular to a method for rapid detection of microorganisms by immunomagnetic bead separation plus ATP bioluminescence technology, belonging to the field of microorganism detection. Background technique: [0002] In recent years, foodborne diseases caused by pathogenic microorganisms have occurred frequently, seriously endangering human health. These foodborne diseases caused by pathogenic microorganisms in food have increasingly high requirements for the identification of pathogenic bacteria. Effective detection of target microorganisms requires rapid, sensitive and specific methods. Although the traditional culture method has high sensitivity and specificity, it usually takes several days or even more than a week to report the detection results. Therefore, the establishment and improvement of rapid detection technology for microorganisms, especially microorganisms in food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543G01N21/76C12Q1/02
Inventor 彭志刚陈国治
Owner 益思美诠生物科技(上海)有限公司
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