Rapid microbiological detection and reagent for environmental water body

A detection method, microbiological technology, applied in the preparation of test samples, chemiluminescence/bioluminescence, analysis by making materials undergo chemical reactions, etc.

Inactive Publication Date: 2005-10-12
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to improve the sensitivity of the current ATP bioluminescence method, eliminate the influence of substances that interfere with the enzyme reaction in the sample on the measurement system, provid

Method used

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  • Rapid microbiological detection and reagent for environmental water body
  • Rapid microbiological detection and reagent for environmental water body
  • Rapid microbiological detection and reagent for environmental water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Rapid detection of the total number of bacteria and the number of microalgae cells in river water

[0039] (1) Sample pretreatment

[0040] a. Refer to the conventional sampling method to take 100mL of river water samples, shake to disperse the samples evenly, and then use a sterilized 20μm microporous filter membrane to vacuum filter the samples to remove particles larger than 20μm;

[0041] b. The water sample is then vacuumed through 8 μm and 0.22 μm microporous membranes in turn, and the filter funnel and the microporous membrane are washed with 50 mL of sterile deionized water;

[0042] (2) ATP extraction from microbial cells

[0043] a. Take the 8μm and 0.22μm microporous filter membranes after filtering the samples, put them in a 50mL sterile beaker, add 1mL sterile deionized water and 1mL microbial cell ATP release agent Ec, shake evenly, and act for 1-5min ;

[0044] b. Take 1 mL of 2×10 Tricine buffer prepared with pH 7.8 and concentration 25 mmo...

Embodiment 2

[0067] Embodiment 2: Rapid detection of the total number of bacteria and the number of microalgae cells in the water storage tank

[0068] (1) Sample pretreatment

[0069] a. Refer to the conventional sampling method to take 1000mL of water samples from the storage tank, and shake to make the samples evenly dispersed;

[0070] b. Vacuumize the water sample through 8 μm and 0.22 μm microporous membranes in turn, and wash the filter funnel and the microporous membrane with 50 mL of sterile deionized water;

[0071] 2. ATP extraction from microbial cells

[0072] a. Take the 8μm and 0.22μm microporous filter membranes after filtering the samples, put them in a 50mL sterile beaker, add 1mL sterile deionized water and 1mL microbial cell ATP release agent Ec, shake evenly, and act for 1-5min ;

[0073] b. Take 1 mL of 2×10 -6 ~2×10 -10 mol / LATP standard solution to replace 1mL sterile deionized water and do the same treatment as the standard sample;

[0074] c. Treat the blank...

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Abstract

A fast detection method of water microbe includes filtering water sample by microhole filtering film with different hole diameter, carrying out ATP luminous detection for used filtering film and for ATP standard solution and new microhole filtering film, calculating linear regression equation of Lg [ATP] = A+ BLg [CPM] based on logarithmic relation of ATP concentration to its luminous pulse counts and calculating out microbe content in water as well as ATP concentration in sample according CPM value of filtering film.

Description

[Affiliated technical field] [0001] The invention relates to a rapid detection method and a reagent for rapid and quantitative detection of the total number of bacteria, microalgae cells and other microorganisms in water bodies such as rivers, lakes, industrial water, domestic drinking water and sea water. [Background technique] [0002] The number of microorganisms in various water bodies such as rivers, lakes, industrial water, drinking water, and seawater is an important indicator for judging their cleanliness. The conventional method is that the staff collect water samples and then bring them back to the laboratory for classification and cultivation. It takes 1 to 15 days to colonize (depending on the type of microorganism), and the operation is cumbersome and limited to the laboratory. It requires high technical level of operators and is prone to human errors. So far, as microorganisms in the natural environment, only a small part can be artificially cultivated, general...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N21/76G06F19/00
Inventor 张菊梅吴清平吴慧清郭伟鹏
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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