Preparation technology of PCL/GE (polycaprolactone/gelatin) electrospinning composite stent

Abstract

The invention discloses a preparation technology of a PCL / GE (polycaprolactone / gelatin) electrospinning composite stent, and belongs to the technical field of biological tissue engineering stents. The preparation technology sequentially comprises a preparation technology of a PCL / GE nanometer fiber electrospinning film which is made of an artificial cell extracellular matrix (ECM) stent material, a preparation method of an electrospinning film coated with hydroxyapatite crystals, and a building method of the electrospinning composite stent. The preparation technology has the advantages that the electrospinning film coated with the hydroxyapatite crystals and the PCL / GE electrospinning film are combined to form an ECM structure which is similar to natural bone tissues; the prepared electrospinning composite stent has no obvious cell toxicity, the internal stent structure is similar to the ECM of the natural bone tissues, and the outer layer structure can block the invasion of peripheral connective tissues; the biocompatibility is good, and the regeneration of bone tissues can be effectively promoted; a stable environment is provided for the regeneration of new bones, and the structure and function of the ECM of the normal bone tissues can be simulated.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering scaffolds, in particular to a preparation process of polycaprolactone / gelatin electrospun composite scaffolds. Background technique [0002] The treatment of bone defects caused by severe trauma, tumor resection, infection, congenital malformation, etc. is a difficult problem and a huge challenge for modern medicine, and it has been an important topic of continuous in-depth research and exploration by humans for centuries. At present, the commonly used repair methods in clinic include autologous bone transplantation, allogeneic bone transplantation and the use of artificial bone, etc., but the above methods all have certain defects: autologous bone transplantation is recognized as the gold standard for bone tissue repair, but patients have to undergo autologous tissue transplantation surgery Therefore, autologous bone grafting cannot be regarded as an ideal repair method for ...

AI Technical Summary

Problems solved by technology

At present, the commonly used repair methods in clinic include autologous bone transplantation, allogeneic bone transplantation and the use of artificial bone, etc., but the above methods all have certain defects: autologous bone transplantation is recognized as the gold standard for bone tissue repair, but patients have to undergo autologous tissue transplantation surgery Therefore, autologous bone grafting cannot be regarded as an ideal repair method for bone defects; there are risks such as immune rejection and disease transmission in allogeneic bone grafting, and sometimes even endanger the patient's life; artificial bone implantation is easy to cause foreign body Rejection, infection, etc.

In contrast, natural materials are not easy to process and have limited physical properties, but natural materials have special biological activities and are generally not easy to trigger immune rejection by the recipient

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